Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 7,692 results for: Lif;

Clear keywords
Showing: 6901 - 6950 of 7692 results

  4. pTC130

    Plasmid
    #91178
    Purpose
    BeYDV T-DNA vector expressing TALENs targeting tomato ANT1, selectable marker gene removed from the backbone (template for amplification of the BaeI containing fragment to create pMOD_C0000)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALENs targeting tomato ANT1
    Use
    TALEN
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91178-plasmid-map-sequence-id-182716

    Enlarge

  10. pFRT-TODestFLAGHA_RBPMS_KDR_K100E

    Plasmid
    #65093
    Purpose
    Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with K100E mutation for stable cell line generation
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    changed lysine 100 to glutamic acid (KDR backgrou…
    Promoter
    CMV, 2x Tet
    Available Since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65093-plasmid-map-sequence-id-110150

    Enlarge

  11. pFRT-TODestFLAGHA_RBPMS_KDR_F65A

    Plasmid
    #65092
    Purpose
    Destination vector with RBPMS resistant to siRNA treatment by s21729 (Applied Biosystems) with F65A mutation for stable cell line generation
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    change phenylalanine 65 to alanine (KDR backbone)
    Promoter
    CMV, 2x Tet
    Available Since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65092-plasmid-map-sequence-id-110149

    Enlarge

  14. pFRT-TODestFLAGHA_RBPMS_KDR_K100A

    Plasmid
    #65663
    Purpose
    FLAG-HA expression vector with RBPMS carrying mutation K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    changed lysine 100 to alanine (KDR background)
    Promoter
    CMV, 2x Tet
    Available Since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65663-plasmid-map-sequence-id-111631

    Enlarge

  17. pFRT-TODestFLAGHA_RBPMS_KDR_F27A

    Plasmid
    #65661
    Purpose
    FLAG-HA expression vector with RBPMS carrying mutation F27A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    changed phenylalanine 27 to alanine (KDR backgrou…
    Promoter
    CMV, 2x Tet
    Available Since
    June 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    65661-plasmid-map-sequence-id-111634

    Enlarge

  29. pM-CBE

    Plasmid
    #196293
    Purpose
    35S promoter-driven expression of the positive-sense TSWV M RNA segment encoding CBE in place of viral GP
    Depositor
    Zhenghe Li
    Article
    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi
    Insert
    Full length TSWV M antigenome encoding CBE in place of viral GP
    Use
    CRISPR
    Tags
    3×flag
    Expression
    Plant
    Promoter
    duplicated cauliflower mosaic virus 35S promoter
    Available Since
    April 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    196293-plasmid-map-sequence-id-389996

    Enlarge

  32. CMV-LUC2CP/ARE

    Plasmid
    #62857
    Purpose
    Splicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.
    Depositor
    Gideon Dreyfuss
    Article
    Younis et al Mol Cell Biol. 2010 Apr;30(7):1718-28. d
    Insert
    Luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    destabilizing sequences (DS) added to the C termi…
    Promoter
    CMV
    Available Since
    April 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    62857-plasmid-map-sequence-id-212900

    Enlarge

  33. pAAV-CAG-LiLac

    Plasmid
    #184570
    Purpose
    Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV
    Depositor
    Gary Yellen
    Article
    Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:
    Insert
    LiLac fluorescent sensor of lactate
    Use
    AAV
    Tags
    His7
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    May 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    184570-plasmid-map-sequence-id-490399

    Enlarge

  43. pDONR P2R-P3-SV40 polyA signal

    Plasmid
    #48627
    Purpose
    Contributes a cassette containing an SV40 polyA signal as the 3’-module during MultiSite Gateway cloning of chimeric cDNAs, when a peptide module at the C-terminal end is not required.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    SV40 early polyadenylation signal cassette
    Use
    Gateway entry vector
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48627-plasmid-map-sequence-id-70632

    Enlarge

  44. pR26

    Plasmid
    #127372
    Purpose
    Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Article
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Promoter
    tight TRE promoter
    Available Since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127372-plasmid-map-sequence-id-247988

    Enlarge

  46. pR26-CMVconst

    Plasmid
    #127373
    Purpose
    Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.
    Depositor
    Lance Miller
    Article
    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Mouse Targeting
    Promoter
    CMV enhancer/promoter
    Available Since
    Feb. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    127373-plasmid-map-sequence-id-248011

    Enlarge

  47. pJRoC30_Tv9nL

    Plasmid
    #188069
    Purpose
    PROSS-designed high-redox potential laccase from Trametes versicolor
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    PROSS-designed high-redox potential laccase from Trametes versicolor
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    79 PROSS mutations, described in publication
    Available Since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188069-plasmid-map-sequence-id-370129

    Enlarge

Showing: 6901 - 6950 of 7692 results