Skip to main content
Addgene
Showing: 521 - 560 of 4899 results
  1. pUCmini-iCAP-AAV9.452sub.LUNG1

    Plasmid
    #184592
    Purpose
    non-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injection
    Depositor
    Insert
    Synthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    7 amino acid substitution between VP1 452 and VP1…
    Promoter
    p41
    Available since
    July 14, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Expression
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pUC19-U6-terminator (SL695)

    Plasmid
    #133961
    Purpose
    pUC19 plasmid with U6 promoter followed by a poly-T termination signal
    Depositor
    Insert
    U6 promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Available since
    Nov. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pUC-GFP-MCC

    Plasmid
    #133307
    Purpose
    "Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisation
    Depositor
    Insert
    microcin-V bacteriocin cassette (cvaC E. coli)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    N112D (please see depositors comments)
    Promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pUC57_beta-globin-5'UTR_Nanoluc

    Plasmid
    #219989
    Purpose
    betaglobin-5'UTR-NanoLuc mRNA synthesis
    Depositor
    Insert
    betaglobin-5'UTR-NanoLuc (HBB Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pUC57-white[coffee]

    Plasmid
    #84006
    Purpose
    Donor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4
    Depositor
    Insert
    A 2,080 bp fragment of the white[coffee] allele (w Fly)
    Use
    Unspecified
    Tags
    Expression
    Mutation
    A GC-to-AA mutation that creates a G589E missense…
    Promoter
    Available since
    Nov. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. pUC19-TSV (linearized in LT)

    Plasmid
    #70033
    Purpose
    pUC19-TSV (linearized in LT) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the Large T-antigen coding region
    Depositor
    Insert
    TSPyV full genome, linearized in Large T-antigen
    Use
    Cloning vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pUC19-P70a-3WJdB-T

    Plasmid
    #87311
    Purpose
    Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.
    Depositor
    Insert
    Three-way Junction dimeric Broccoli aptamer
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P70a
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pUC57-GFP-SFPQ-RT

    Plasmid
    #97090
    Purpose
    Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.
    Depositor
    Insert
    EGFP-SFPQ-RT (SFPQ Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 31, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 521 - 560 of 4899 results