We narrowed to 5,097 results for: puc
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Plasmid#185137Purposenon-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-MaCPNS2 VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-gCarvi-T2A
Plasmid#190333PurposeFluorescent reporter for cAMPDepositorInsertgCarvi
UseUnspecifiedAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV9.452sub.LUNG1
Plasmid#184592Purposenon-standard AAV2 rep-AAV9.452sub.LUNG1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system for mouse lung transduction after intravenous injectionDepositorInsertSynthetic construct isolate AAV9.452sub.LUNG1 VP1 gene
UseAAVExpressionMammalianMutation7 amino acid substitution between VP1 452 and VP1ā¦Promoterp41Available SinceJuly 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19/PLIN2-GFP
Plasmid#202194Purposeknock-in donor vector of GFP to the C-terminus of endogenous human PLIN2DepositorAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-AAV1.X1
Plasmid#209780Purposenon-standard AAV2 rep-AAV1-X1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV1-X1 VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-hNIL
Plasmid#105841PurposeDonor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuronDepositorInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-MCC
Plasmid#133307Purpose"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisationDepositorInsertmicrocin-V bacteriocin cassette (cvaC E. coli)
UseSynthetic BiologyExpressionBacterialMutationN112D (please see depositors comments)Available SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B
Plasmid#103002Purposenon-standard AAV2 rep-AAV-PHP.B cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18R6K-mini-Tn7T-Gm
Plasmid#65022PurposeGmR mini-Tn7 vector, R6K repliconDepositorTypeEmpty backboneAvailable SinceAug. 17, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUCmini-iCAP-AAV.MaCPNS1
Plasmid#185136Purposenon-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-MaCPNS1 VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.N
Plasmid#127851Purposenon-standard AAV2 rep-AAV-PHP.N cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.N VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19 Giles integrase
Plasmid#170049PurposepUC19 with Giles integraseDepositorInsertGIles Integrase
ExpressionBacterialAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET259-pUC57 24xMS2V6
Plasmid#104391PurposeMS2V6 cloningDepositorInsert24xMS2V6
ExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucleā¦Available SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.eC
Plasmid#201903Purposenon-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.eC VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC gRNA Shuttle
Plasmid#47024PurposeEncodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.DepositorInsertgRNA Shuttle
UseCRISPR; Cas9ExpressionPlantMutationG to T cloning mutation at position 323PromoterMedicago truncatula U6.6Available SinceSept. 3, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19-hAPOE-R-loop
Plasmid#134901PurposeGeneration and purification of R-loops for in vitro biochemistryDepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19_dTAG-KANSL3-targeting
Plasmid#170755PurposeDonor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locusDepositorInsertBSD-P2A-2xHA-dTAG
UseMouse TargetingTagsBSD-P2A-2xHA-dTAGMutationPhenylalanine 36 to valine in FKBP12Available SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.AX
Plasmid#195218PurposeAAV capsid for broadly targeting cell types in the CNS (neurons including both excitatory and inhibitory neurons, astrocytes)DepositorInsertSynthetic construct isolate AAV-PHP.AX VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.V1
Plasmid#127847Purposenon-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.V1 VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC18R6K-mini-Tn7T
Plasmid#64958Purposemini-Tn7 base vector with transcriptional terminatorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18-mini-Tn7
Plasmid#64953Purposemini-Tn7 base vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-rm_gCarvi-T2A
Plasmid#190334PurposeRatiometric reporter for cAMPDepositorInsertratiometric gCarvi
UseUnspecifiedAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC-hrDNA-12.0
Plasmid#206320PurposeA plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)DepositorInsertIntergenic spacer of human rDNA repeat
UseCloningAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19 PKC delta
Plasmid#8427DepositorInsertMouse Protein Kinase C delta (Prkcd Mouse)
ExpressionBacterialAvailable SinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUC18/promEco/Lysostaphin
Plasmid#125765PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
ExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK-Sa1 promoterAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19 PKC alpha
Plasmid#8429DepositorInsertMouse Protein Kinase C alpha (Prkca Mouse)
ExpressionBacterialAvailable SinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B5
Plasmid#172846PurposeCRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19/NLS-mChe
Plasmid#183162PurposeConstruct for transient expression of cell nucleus-targeted mCherry in plants.DepositorInsertNLS-mCherry
Tags16 aa nuclear localization signal and 16 aa nuclā¦ExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC-AdEvolve-DEST
Plasmid#131692PurposeA version of AdEvolve-DEST with a high copy origin for easier DNA preparation and transfection.DepositorTypeEmpty backboneUseAdenoviralAvailable SinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B1
Plasmid#172842PurposeCRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mEGFP
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Golgi
Plasmid#183169PurposeConstruct for transient expression of Golgi apparatus-targeted mCherry in plants.DepositorInsertmCherry-Golgi
TagsAtRER1B, A. thaliana endoplasmic reticulum retrieā¦ExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T
Plasmid#64954Purposemini-Tn7 base vector with transcriptional terminatorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-sgRNA-MS2-U6
Plasmid#171694PurposeTo construct expression vector for tBE-V5-mA3DepositorInsertsgRNA scaffold-MS2-U6
UseCRISPRExpressionMammalianAvailable SinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-GW
Plasmid#64961Purposemini-Tn7 Gateway destination vectorDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18T-mini-Tn7T-Tp
Plasmid#65024PurposeTpR mini-Tn7 vector, mobilizableDepositorTypeEmpty backboneAvailable SinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSLQ5429_pUC_hU6-crScaffold_EF1a-BFP
Plasmid#155306PurposeRfx Cas13d guide cloning pUC19 vector with constitutively expressed tagBFPDepositorInsertRfx Cas13d CRISPR RNA BFP
ExpressionMammalianPromoterhU6, EF1aAvailable SinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC18/promSau/Lysostaphin
Plasmid#125766PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
ExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK constitutive promoterAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-3WJdB-T
Plasmid#87311PurposeExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.DepositorInsertThree-way Junction dimeric Broccoli aptamer
UseSynthetic BiologyExpressionBacterialPromoterP70aAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B3
Plasmid#103004Purposenon-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B3 VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B2
Plasmid#103003Purposenon-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B2 VP1 gene
UseAAVExpressionMammalianPromoterp41Available SinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only