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We narrowed to 1,740 results for: plasmid for e coli

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Showing: 651 - 700 of 1740 results

  2. pLVX-TetOne-Puro-mitoEcSTH-FLAG

    Plasmid
    #218629
    Purpose
    This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.
    Depositor
    Valentin Cracan
    Article
    Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038
    Insert
    mitoEcSTH
    Use
    Lentiviral and Synthetic Biology
    Tags
    FLAG and MTS
    Expression
    Mammalian
    Available Since
    May 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    218629-plasmid-map-sequence-id-432199

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  8. pNIC28-Bsa4-TF

    Plasmid
    #61689
    Purpose
    E. coli expression vector based on pNIC28-Bsa4 (addgene #26103) with the TEE and trigger factor sequences inserted
    Depositor
    Brian Smith
    Type
    Empty backbone
    Tags
    HIS and Trigger Factor
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. Sc FLAG-S6-PCNA-pET

    Plasmid
    #239471
    Purpose
    Overexpress Sc FLAG-S6-PCNA in E. coli
    Depositor
    Michael O'Donnell
    Insert
    Sc FLAG-S6-PCNA
    Tags
    3X FLAG followed by S6
    Expression
    Bacterial
    Available Since
    June 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    239471-plasmid-map-sequence-id-475695

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  10. pRF0G-Tyr

    Plasmid
    #113644
    Purpose
    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    MjYRS and tRNA gene
    Use
    Synthetic Biology
    Available Since
    July 27, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    113644-plasmid-map-sequence-id-217129

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  13. pFN1-catP-oriT

    Plasmid
    #244451
    Purpose
    A pFN1-aad9 plasmid carrying an OriT sequence for conjugative transfer into F. nucleatum.
    Depositor
    Xue Liu
    Insert
    CatP
    Expression
    Bacterial
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    244451-plasmid-map-sequence-id-490026

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  14. pFN1-Aad9

    Plasmid
    #244449
    Purpose
    A shuttle plasmid compatible with Fusobacterium nucleatum ATCC10953 or ATCC25586, carrying a spectinomycin resistance marker (aadA gene).
    Depositor
    Xue Liu
    Inserts
    pFn1 Fn ori
    Aad9
    Expression
    Bacterial
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    244449-plasmid-map-sequence-id-490024

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  15. p46Cpf1-OP2

    Plasmid
    #98592
    Purpose
    Produces lambda Red and Cpf1 for recombination and selection. The plasmid is combined with a donor plasmid (with crRNA and template) for rapid genome editing in E.coli.
    Depositor
    Qiong Wu
    Article
    Ao et al Front Microbiol. 2018 Oct 9;9:2307. doi:
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Codon optimized for E. coli
    Promoter
    araBAD
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    98592-plasmid-map-sequence-id-201329

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  16. pCVD419

    Plasmid
    #63754
    Purpose
    3.4 kb HindIII fragment from EHEC associated plasmid from E. coli 0157:H7 strain 933 cloned into pBR325. Contains the hlyA gene. Best EHEC probe for detection of this plasmid.
    Depositor
    James Kaper
    Article
    Levine et al J Infect Dis. 1987 Jul;156(1):175-82.
    Insert
    ehxA
    Expression
    Bacterial
    Available Since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. COVID-SARS2 NSP14/10

    Plasmid
    #159613
    Purpose
    Bacterial co-expression vector fo Covid-SARS2 NSP14 and NSP10
    Depositor
    Opher Gileadi
    Article
    Baddock et al Nucleic Acids Res. 2022 Jan 17. pii: 650
    Inserts
    Covid-SARS2 Nsp14 (ORF1ab COVID-SARS2, Synthetic)
    Covid-SARS2 Nsp10
    Tags
    His6, TEV cleavage site and none
    Expression
    Bacterial
    Mutation
    Codon-optimized for E. coli expression
    Promoter
    (Bicistronic) and T7 - LacO
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159613-plasmid-map-sequence-id-311056

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  18. pBsacA

    Plasmid
    #158650
    Purpose
    Constitutive transcription of sacA crRNA
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    Pveg
    Available Since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158650-plasmid-map-sequence-id-314145

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  19. pHTganA-Cpf1

    Plasmid
    #158647
    Purpose
    Constitutive transcription of FnCpf1 and ganA crRNA
    Depositor
    Wenjing Cui
    Insert
    Cpf1, ganA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    P43
    Available Since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158647-plasmid-map-sequence-id-314144

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  20. pADsacA

    Plasmid
    #158649
    Purpose
    Constitutive transcription of sacA crRNA and GFP
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Expression
    Bacterial
    Promoter
    Pveg
    Available Since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158649-plasmid-map-sequence-id-310569

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  21. pET32a-LIC

    Plasmid
    #62310
    Purpose
    E. coli expression vector with N-terminal Trx-tag, 6X His-tag, thrombin site, S-tag, and TEV
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis, S-tag, and Trx-Tag
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62310-plasmid-map-sequence-id-326570

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  22. pACYC-LIC+

    Plasmid
    #62312
    Purpose
    E. coli pACYC based expression vector with N-terminal Hexa-HIS and a thrombin protease cleavage site
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and thrombin protease cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pACYC-GST

    Plasmid
    #62329
    Purpose
    E. coli pACYC based expression vector with N-terminal TEV cleavable GST-tag
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    Glutathione S-transferase (GST) tag and TEV prote…
    Expression
    Bacterial
    Promoter
    T7-lac0
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62329-plasmid-map-sequence-id-296399

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  24. Sc FLAG-S6-rad24-pRSFDuet

    Plasmid
    #240734
    Purpose
    Over-express FLAG-S6-rad24 in E. coli
    Depositor
    Michael O'Donnell
    Insert
    FLAG-S6-rad24 (RAD24 Budding Yeast)
    Tags
    3X FLAG followed by S6
    Expression
    Bacterial
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240734-plasmid-map-sequence-id-482405

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  25. Sc [ddc1 + rad17]-pET

    Plasmid
    #240740
    Purpose
    Over-express Sc ddc1 & Sc rad17 in E. coli
    Depositor
    Michael O'Donnell
    Inserts
    ddc1 (DDC1 Budding Yeast)
    rad17 (RAD17 Budding Yeast)
    Expression
    Bacterial
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240740-plasmid-map-sequence-id-482365

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  26. Sc Rad27-S6- pET24

    Plasmid
    #239205
    Purpose
    Overexpress Sc Rad27 (FEN1) (C-terminal S6 tag) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    Rad27 (RAD27 Budding Yeast)
    Tags
    S6
    Expression
    Bacterial
    Available Since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239205-plasmid-map-sequence-id-474608

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  27. Sc FLAG-RFC1-pRSFDuet

    Plasmid
    #239201
    Purpose
    Overexpress Sc RFC1 (N-terminal 3X FLAG tag) in E. coli
    Depositor
    Michael O'Donnell
    Insert
    RFC1 (RFC1 Budding Yeast)
    Tags
    3X FLAG
    Expression
    Bacterial
    Available Since
    June 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239201-plasmid-map-sequence-id-474602

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  28. StarShip01

    Plasmid
    #164505
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains T7-HaloTag-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Availability
    Academic Institutions and Nonprofits only
    164505-plasmid-map-sequence-id-324722

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  29. StarShip02

    Plasmid
    #164506
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES(Myb)-T7-Halo-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Availability
    Academic Institutions and Nonprofits only
    164506-plasmid-map-sequence-id-324723

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  30. StarShip03

    Plasmid
    #164507
    Purpose
    To generate site specific attP-GA integrant cell lines upon co-delivery with BxbI. Contains IRES2-T7-Halo-PDGFR_TM-T2A-Puro.
    Depositor
    Bo Huang
    Type
    Empty backbone
    Use
    Bxbi site specific integration
    Availability
    Academic Institutions and Nonprofits only
    164507-plasmid-map-sequence-id-324724

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  31. pLK06Hpp-Fab CR3009

    Plasmid
    #239861
    Purpose
    Plasmid for simultaneous Fab and GFP expression from separate Lac promoters for online monitoring of induction with a GFP sensor station. Fab is expressed as a fusion with alkaline phosphatase.
    Depositor
    Urpo Lamminmäki
    Article
    Korkiakoski et al Protein Expr Purif. 2025 Aug 28:106808.
    Inserts
    Fab (antigen binding fragment) anti-nucleocapsid protein of SARS-COV viruses 1 and 2 (N, N Human)
    GFP with F64L, S65T, Q80R, M153T and V163A mutations
    Tags
    Hexahistidine tag and bacterial alkaline phosphat…
    Expression
    Bacterial
    Mutation
    F64L, S65T, Q80R, M153T and V163A
    Promoter
    Lac promoter
    Available Since
    Sept. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239861-plasmid-map-sequence-id-485412

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  32. pTuLys2CMR2

    Plasmid
    #53544
    Purpose
    Inhibition module. Constructed from the parent plasmids pLuxRI, pLuxmCherry, pET15bLCFPT7, and pLysS. The plasmid can express T7 lysozyme by inducing AHL and LuxR. Chloramphenicol resistance
    Depositor
    Lingchong You
    Article
    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10
    Insert
    pLux
    Use
    Unspecified
    Available Since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    53544-plasmid-map-sequence-id-83437

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  33. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available Since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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Showing: 651 - 700 of 1740 results