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pICSL1P3_AtU626Ter26

Plasmid

#191771

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL11197

Plasmid

#191784

Purpose

SpCas9 expression in plants

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

AtUbi10::Cas9 +13 introns-T-E9

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-prepore

Plasmid

#160519

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-prepore

Tags

His-Tag

Expression

Bacterial

Mutation

I104C, G244C

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-WT-Y336W

Plasmid

#160515

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-Y336W

Tags

His-Tag

Expression

Bacterial

Mutation

Y336W

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRS303-pGAL-SPPHO5-G-SrtAstrep-HA-HDEL (pKS82)

Plasmid

#50032

Purpose

Galactose-inducible expression of ER-luminal S. pyogenes SrtA in yeast

Depositor

Hidde Ploegh

Article

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

HA-SrtA(strep)

Tags

HA-HDEL

Expression

Yeast

Promoter

pGAL

Available Since

Jan. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0016

Plasmid

#117526

Purpose

Level0 Golden Gate part: CDS, Sp-eCas9 1.0

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

Sp-eCas9 1.0 (with stop codon)

Use

CRISPR

Mutation

K810A, K1003A, R1060A

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pHS-dCas9

Plasmid

#240212

Purpose

Mammalian vector for transient expression of dCas9

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

dCas9

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

PGK

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pICSL1P4_AtU626Ter26

Plasmid

#191772

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P7_AtU626Ter26

Plasmid

#191775

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P2_AtU626Ter26

Plasmid

#191770

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL1P5_AtU626Ter26

Plasmid

#191773

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pICSL1P6_AtU626Ter26

Plasmid

#191774

Purpose

Cas9 guide expression in dicots

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Insert

SpCas9 guide scaffold with AtU626 promoter/terminator

Use

CRISPR

Expression

Plant

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Yeast_Target-AID2S

Plasmid

#188647

Purpose

Expressing Target-AID2S in yeasts

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nCas9-AID2S-UGI

Tags

UGI

Expression

Yeast

Mutation

D10A for SpCas9

Promoter

pGal1

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-N342W

Plasmid

#160536

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, N342W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-L340W

Plasmid

#160535

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-L340W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, L340W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-N338W

Plasmid

#160534

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-N338W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, N338W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-Y336W

Plasmid

#160533

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-Y336W

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, Y336W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock-charge+

Plasmid

#160531

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C, Y336K, L340K

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock

Plasmid

#160530

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB1lock-N342W

Plasmid

#160529

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Article

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock-N342W

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C, N342W

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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