We narrowed to 2,526 results for: T2A

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pPB_mU6_enh-gRNA_Puro-T2A-BFP

Plasmid

#235571

Purpose

Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

sgRNA backbone

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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pL7Ae-myc-T2A-tagRFP

Plasmid

#140263

Purpose

Expresses L7Ae in mammalian cells

Depositor

Hirohide Saito

Article

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

L7Ae

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CEP41-HaloTag7_T2A_EGFP

Plasmid

#169327

Purpose

Expression of CEP41-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag7 (CEP41 Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pTet-O-ASCL1-T2A-PuroR

Plasmid

#162345

Purpose

Lentiviral expression of ASCL1 under the control of the TetON promoter

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ASCL1 (ASCL1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FlipCherry-T2A-GFP

Plasmid

#124436

Purpose

Expresses FlipCherry (TEV cleavage sequence) and T2A GFP in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipCherry-T2A-GFP

Expression

Mammalian

Available Since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloAKAR2.1-T2A-EGFP

Plasmid

#216489

Purpose

Expression of chemigenetic PKA biosensor (medium basal brightness, high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloAKAR2.1

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas12i2-T2A-EGFP

Plasmid

#188270

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Chen et al Innovation (Camb). 2022 May 26;3(4):1002

Insert

Cas12i2

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

CAG

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pME-Cas9-T2A-GFP

Plasmid

#63155

Purpose

Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Insert

Cas9-T2A-GFP

Use

CRISPR; Tol2 middle entry vector for gateway

Available Since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG-mNaChBac-T2A-tdTomato

Plasmid

#60650

Purpose

Expresses mNaChBac-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

mNaChBac-T2A-tdTomato

Expression

Mammalian

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_miniSOG2 T2A H2B-EGFP

Plasmid

#87410

Purpose

Mammalian expression vector encoding Histone 2B GFP fusion and miniSOG2

Depositor

Xiaokun Shu

Article

Makhijani et al Cell Chem Biol. 2017 Jan 19;24(1):110-11

Insert

mini singlet oxygen generator 2

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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EF1a-L274GMmMetRS-T2A-mCherry

Plasmid

#220800

Purpose

Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

Depositor

Elizabeth Kirby

Article

Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

Insert

Mars1 (Mars1 Mouse)

Use

Lentiviral

Tags

2xFLAG and T2A-mCherry

Mutation

mutation in MetRS to change aa 274 from L to G

Promoter

EF1a

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iCasper T2A HO1

Plasmid

#64278

Purpose

Expresses infrared fluorescent executioner caspase reporter (iCasper) plus human heme oxygenase 1

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent executioner caspase reporter (iCasper) T2A heme oxygenase-1: (HMOX1 Synthetic)

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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PB CAG-mTagBFP2-T2A-iCreERT2

Plasmid

#177308

Purpose

CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon

Depositor

Mario Capecchi

Insert

mTagBFP2-T2A-iCreERT2

Use

Cre/Lox; Piggybac

Expression

Mammalian

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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FUGW-APPSwe-T2A-mCherry

Plasmid

#190804

Purpose

Express Swedish mutant APP from Ubiquitin C promoter, co-expressing mCherry

Depositor

Ronald Hart

Article

Ma et al J Neurosci. 2023 Mar 8;43(10):1830-1844.

Insert

APP (APP Human)

Use

Lentiviral

Mutation

Swedish (KM670/671NL)

Promoter

Ubi C

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-T2A-Venus(L68V)

Plasmid

#60494

Purpose

Produces equimolar levels of mTurquoise2 and mVenus(L68V). It can be used as a negative control for FRET.

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2-T2A-mVenus(L68V)

Tags

T2A peptide

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymNeongreen-T2A-mCherry

Plasmid

#125700

Purpose

Produces equimolar expression of yeast codon optimized mNeongreen and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymNeongreen-T2A-mCherry

Expression

Yeast

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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FUGW-APPwt-T2A-mCherry

Plasmid

#190803

Purpose

Express wild-type APP from Ubiquitin C promoter, co-expressing mCherry

Depositor

Ronald Hart

Insert

APP (APP Human)

Use

Lentiviral

Promoter

Ubi C

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-MYC-t2A-BCL2

Plasmid

#135306

Purpose

Overexpression of multiple human genes (BCL2 and MYC)

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

MYC (MYC Human)
BCL2 (BCL2 Human)

Use

Retroviral

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Kir2.1Mut-T2A-tdTomato

Plasmid

#60644

Purpose

Expresses Kir2.1Mut-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kcnj2 (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

GYG to AAA (aa144-146)

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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EasyFusion T2A-H2B-mCherry

Plasmid

#113089

Purpose

General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

T2A-H2B-mCherry

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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