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Plasmid#135671PurposeIntroduce constitutive (EF1a promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchangeDepositorInsertGFP
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_CD WT TET1
Plasmid#124082PurposeExpression of wild-type catalytic domain only form of TET1DepositorInsertTET1 catalytic domain (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterEF1 alphaAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSMPUW_6.5_M2_Ef1a-V5-gateway
Plasmid#128441Purposelentiviral destination vector with Ef1a promoter and N-terminal V5 tagDepositorInsertEf1a promoter and N-terminal V5 tag
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaCTD_P2A_Hygro_Barcode
Plasmid#120511PurposeBarcoded lentiviral vector to express MYC deltaCTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaCTD (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of carboxy-terminal domainPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaNTD_P2A_Hygro_Barcode
Plasmid#120510PurposeBarcoded lentiviral vector to express MYC deltaNTD in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaNTD (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of amino-terminal domainPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaMBI_P2A_Hygro_Barcode
Plasmid#120504PurposeBarcoded lentiviral vector to express MYC deltaMBI in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltaMBI (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of MYC Box I domainPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltab_P2A_Hygro_Barcode
Plasmid#120507PurposeBarcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC deltab (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of basic motifPromoterEF1aAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaMBII_P2A_Hygro_Barcode
Plasmid#120505PurposeBarcoded lentiviral vector to express c-MYC deltaMBII in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertc-MYC deltaMBII (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of MYC Box II domainPromoterEF1aAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC deltaNLS_P2A_Hygro_Barcode
Plasmid#120506PurposeBarcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC H84NLS (MYC Human)
UseLentiviralTagsExpressionMutationDeletion of nuclear localization signal sequencePromoterEF1aAvailable sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-hEF1A-MG-U6-sgRNA
Plasmid#175505PurposeAll-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhEF1a, U6Available sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->HA-mtC11L34
Plasmid#162227PurposeLentiviral vector for the expression of HA mtC11L34 in mammalian cellsDepositorInsertmtC11L34
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPL5628_TEF1*-Cre_MET15
Plasmid#60933PurposeConstitutive expression vector for Cre-recombinase containing MET15 selection marker.DepositorInsertCre
UseTagsDriven from a mutant TEF1 promoter with 0.16x act…ExpressionYeastMutationPromotermutant TEF1Available sinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSNR52-sgTEF1
Plasmid#46922PurposeYeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoterDepositorInsertsgTEF1 promoter (TEF1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYC Thr58Ala_P2A_Hygro_Barcode
Plasmid#120513PurposeBarcoded lentiviral vector to express MYC Thr58Ala in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYC Thr58Ala (MYC Human)
UseLentiviralTagsExpressionMutationPoint mutation changing Threonine to Alanine at a…PromoterEF1aAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1_Cre
Plasmid#201198PurposeAAV vector for cre expression under EF1 promoterDepositorInsertscre
EF1alpha promoter
UseAAVTagsExpressionBacterial and MammalianMutationnuclear targeting crePromoterN/A and hEF1alpha promoterAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EEF1A2_GTP-EFTU
Plasmid#109862PurposeProtein expression and purification of EEF1A2_GTP-EFTUDepositorInsertEEF1A2_GTP-EFTU (EEF1A2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF1p_mFAP2b
Plasmid#159469PurposeEncodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1p_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceDec. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-EF1p3_mFAP2b
Plasmid#159470PurposeEncodes for EF1p3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.DepositorInsertEF1p3_mFAP2b
UseTags6xHis-thrombin-TEVExpressionBacterialMutationPromoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-mRor2N620K
Plasmid#22631DepositorInsertRor2 N620K (Ror2 Mouse)
UseTagsFlagExpressionMammalianMutationChanged Asparagine 620 to LysinePromoterAvailable sinceDec. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_EEF1A1_GTP-EFTU
Plasmid#109861PurposeProtein expression and purification of EEF1A1_GTP-EFTUDepositorInsertEEF1A1_GTP-EFTU (EEF1A1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only