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pAL119-TK

Plasmid

#21911

Depositor

Maria Castro

Article

Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

Insert

HSV Type 1 thymidine kinase

Use

Adenoviral

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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iRFP-P4M-SidM

Plasmid

#51470

Purpose

Biosensor for PtdIns4P

Depositor

Tamas Balla

Article

Hammond et al J Cell Biol. 2014 Apr 7.

Insert

P4M-SidM(546-647)

Tags

iRFP

Expression

Mammalian

Promoter

CMV

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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mEGFP-ER-5a

Plasmid

#56455

Purpose

Localization: Endoplasmic Reticulum, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

ER 5a (CALR Human)

Tags

mEGFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3.1-Myoferlin HA

Plasmid

#22443

Depositor

William Sessa

Article

Bernatchez et al J Biol Chem. 2007 Oct 19. 282(42):30745-

Insert

Myoferlin

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

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gRNA_AAVS1-T2

Plasmid

#41818

Purpose

Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_AAVS1-T2

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pBa.TfR.GFP

Plasmid

#45060

Depositor

Gary Banker, Marvin Bentley

Article

Burack et al Neuron. 2000 May;26(2):465-72.

Insert

TfR (TFRC Human)

Tags

GFP

Expression

Mammalian

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR5'_ubi

Plasmid

#27320

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

zebrafish ubiquitin promoter (ubb Zebrafish)

Use

Multisite gateway 5' entry vector

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

Plasmid

#171588

Purpose

Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

Bxb1 integrase
iCasp9
BFP
rtTA3G

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCRISPR-HOT_tdTomato

Plasmid

#138567

Purpose

Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

Depositor

Hans Clevers

Article

Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

Insert

tdTomato

Use

Unspecified

Available Since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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DT960

Plasmid

#80412

Purpose

Expresses 960 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

Depositor

Thomas Cooper

Article

Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

Insert

human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)

Expression

Mammalian

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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phL1A-pcDNA3

Plasmid

#12307

Depositor

Vance Lemmon

Article

Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

Insert

L1CAM (L1CAM Human)

Expression

Mammalian

Available Since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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pCAG-FlpO

Plasmid

#89574

Purpose

Expresses FlpO under pCAG

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

FlpO

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Available Since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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p1321 HPV-16 E6/E7

Plasmid

#8641

Depositor

Peter Howley

Article

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Insert

HPV-16 E6/E7 (E7, E6 Human papillomavirus)

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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pCI SMN2

Plasmid

#72287

Purpose

SMN2 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN2 (SMN2 Human)

Expression

Mammalian

Mutation

contains exons 6-7-8 including introns, multiple …

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S CK2M EN2L

Plasmid

#20924

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Klf4 and Myc; Nanog and Lin28

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Lin28; Klf4 and Myc (MYC, SOX2, KLF4, NANOG, POU5F1, LIN28A Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pMSP1E3

Plasmid

#20064

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E3 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeats of he…

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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His6-MBP-uTEV3

Plasmid

#135464

Purpose

Bacterial expression of uTEV3 (full-length) protease

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

His6-MBP-uTEV3

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation (improves stability) , I138T/S153N…

Promoter

T5

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pR26 CAG/GFP Asc

Plasmid

#74285

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette and GFP, for cloning into AscI

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Mouse Targeting

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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IGF1R_pLX307

Plasmid

#98344

Purpose

Lentiviral expression of IGF1R

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

IGF1R (IGF1R Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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AAV phSyn1(S)-DreO-bGHpA

Plasmid

#50363

Purpose

Can be used to generate AAV virus that will express DreO recombinase in neurons from the synapsin promoter

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Has Service

AAV Retrograde and AAV5

Insert

DreO recombinase

Use

AAV

Tags

none

Mutation

Sequence optimized for expression in mammalian ce…

Promoter

hSyn1

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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