We narrowed to 11,558 results for: NSI

1. pHcPro (GB0067)

   Plasmid

   #68196

   Purpose

   Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   TEV HcPro coding region
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pKDsgRNA-hisG

   Plasmid

   #89954

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   hisG gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHH0103_SH3RF1-1/4

   Plasmid

   #91544

   Purpose

   Protein expression and purification of human SH3 domain construct SH3RF1-1/4

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3RF1-1/4 (SH3RF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHH0103_SH3RF1-2/4

   Plasmid

   #91457

   Purpose

   Protein expression and purification of human SH3 domain construct SH3RF1-2/4

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3RF1-2/4 (SH3RF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHH0103_SH3RF1-4/4

   Plasmid

   #91374

   Purpose

   Protein expression and purification of human SH3 domain construct SH3RF1-4/4

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3RF1-4/4 (SH3RF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHH0103_SH3RF3-2/2

   Plasmid

   #91356

   Purpose

   Protein expression and purification of human SH3 domain construct SH3RF3-2/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3RF3-2/2 (SH3RF3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHH0103_SH3RF1-3/4

   Plasmid

   #91366

   Purpose

   Protein expression and purification of human SH3 domain construct SH3RF1-3/4

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3RF1-3/4 (SH3RF1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHH0103_SH3RF3-1/2

   Plasmid

   #91280

   Purpose

   Protein expression and purification of human SH3 domain construct SH3RF3-1/2

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   SH3RF3-1/2 (SH3RF3 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pXPR_BRD003 sgLacZ-1

   Plasmid

   #74968

   Purpose

   sgLacZ-1, puromycin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Insert

   LacZ
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXPR_BRD003 sgLacZ-2

    Plasmid

    #74969

    Purpose

    sgLacZ-2, puromycin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    LacZ
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BAS291 C terminal mKO2 kanR

    Plasmid

    #74086

    Purpose

    S.pombe recoded mKO2 in universal C-terminal tagging plasmid, G418 resistance.

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    mKO2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pLLOXmPOLBeta

    Plasmid

    #24174

    Depositor

    Robert Sobol

    Article

    Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. do

    Insert

    pLLOXmPOLbeta (Polb Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    x

    Promoter

    Available Since

    May 27, 2010

    Availability

    Academic Institutions and Nonprofits only

13. HM3C NaVSp1_p pET24b

    Plasmid

    #127852

    Purpose

    Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVSp1
    

    Use

    Tags

    3C Cleavage site, His Tag, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pY71-tet.I.16

    Plasmid

    #133536

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHH0103_SH3BP4-1/1

    Plasmid

    #91532

    Purpose

    Protein expression and purification of human SH3 domain construct SH3BP4-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SH3BP4-1/1 (SH3BP4 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHH0103_SORBS2-1/3

    Plasmid

    #91387

    Purpose

    Protein expression and purification of human SH3 domain construct SORBS2-1/3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    SORBS2-1/3 (SORBS2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHH0103_VAV1-1/2

    Plasmid

    #91355

    Purpose

    Protein expression and purification of human SH3 domain construct VAV1-1/2

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    VAV1-1/2 (VAV1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMM549

    Plasmid

    #112531

    Purpose

    pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR

    Depositor

    Timothy Lu

    Article

    Mimee et al Science. 2018 May 25;360(6391):915-918.

    Inserts

    HrtR
    ChuA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23107 and ProD

    Available Since

    Feb. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. BAS290 C terminal E2C natR

    Plasmid

    #74085

    Purpose

    S.pombe recoded E2Crimson in universal C-terminal tagging plasmid, nourseothricin resistance

    Depositor

    Bassem Al-Sady

    Article

    Al-Sady et al PLoS One. 2016 Aug 1;11(8):e0159292. doi

    Insert

    E2Crimson
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pHH0103_STAM-1/1

    Plasmid

    #91428

    Purpose

    Protein expression and purification of human SH3 domain construct STAM-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    STAM-1/1 (STAM Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pFastBac Dual PNG-FLAG PLU-HIS

    Plasmid

    #113173

    Purpose

    Baculovirus expression of PNG and PLU

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    PNG, PLU
    

    Use

    Baculovirus

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBad-CA TAG233

    Plasmid

    #105838

    Purpose

    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 233 and C-terminal 6x his tag

    Depositor

    Ryan Mehl

    Article

    Mehl et al 2020

    Insert

    human carbonic anhydrase II thermostable variant (CA2 Human)
    

    Use

    Tags

    6x his tag

    Expression

    Bacterial

    Mutation

    TAG stop codon at site 233

    Promoter

    pBad/arabinose

    Available Since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. sh-Myocardin

    Plasmid

    #100768

    Purpose

    expression of shRNA targeting MYOCD

    Depositor

    Joseph Gordon

    Article

    Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

    Insert

    sh-Myocardin
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    H1

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHH0103_PACSIN3-1/1

    Plasmid

    #91335

    Purpose

    Protein expression and purification of human SH3 domain construct PACSIN3-1/1

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

    Insert

    PACSIN3-1/1 (PACSIN3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pcDNA3.1-RsACR_995-EYFP

    Plasmid

    #103772

    Purpose

    Anion channelrhodopsin from Rhodomonas salina (CCMP1319) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin RsACR_995
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA3.1-GcACR_457-EYFP

    Plasmid

    #103137

    Purpose

    Anion channelrhodopsin from Geminigera cryophila (CCMP2564) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin GcACR_457
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. M26V DJ-1 pET15b

    Plasmid

    #60688

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26V DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26V

    Promoter

    T7

    Available Since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pKDsgRNA-trmI

    Plasmid

    #89955

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting trmI.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    trmI gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJW3.42

    Plasmid

    #62866

    Purpose

    Yeast two-hybrid bait vector expressing LexA-PPTR-1 fusion

    Depositor

    Geraldine Seydoux

    Article

    Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

    Insert

    PPTR-1/pLexA-N bait
    

    Use

    Two-hybrid bait vector

    Tags

    LexA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTAtUbq3 (GB0219)

    Plasmid

    #68192

    Purpose

    Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtUbq3 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available Since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAB085d7

    Plasmid

    #79211

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ppro1 434cI-TnCAD-F3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAB085d6

    Plasmid

    #79210

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1) luxAB; Ppro1 434cI-TnTBR3-F3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PlacZ-opt (OR1); Ppro1

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. PFA0445w-bio-His

    Plasmid

    #50811

    Purpose

    Expresses enzymatically monobiotinylated full length PFA0445w

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    PFA0445w
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PF10_0166-bio-His

    Plasmid

    #50801

    Purpose

    Expresses enzymatically monobiotinylated full length PF10_0166

    Depositor

    Gavin Wright

    Article

    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1

    Insert

    PF10_0166
    

    Use

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMXs-B3

    Plasmid

    #31707

    Depositor

    Duanqing Pei

    Article

    Liao et al J Biol Chem. 2011 May 13. 286(19):17359-

    Insert

    miR-20b (Mir20b Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA3.1-R1ACR_741-EYFP

    Plasmid

    #103961

    Purpose

    Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCMP768) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin R1ACR_741
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA4-Tet-on-vsv-Incenp

    Plasmid

    #108480

    Purpose

    inducible expression of vsv-INCENP wt

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    INCENP (INCENP Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pDONR-IAA17_no stop

    Plasmid

    #83855

    Purpose

    Gateway donor vector for IAA17 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. TDP43 NOTAG11

    Plasmid

    #28210

    Depositor

    Zuoshang Xu

    Article

    Yang et al PLoS One. 2010 . 5(12):e15878.

    Insert

    Tar DNA binding protein (TARDBP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    aa193-274 only

    Promoter

    Available Since

    Sept. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

40. pKDsgRNA-pfkB

    Plasmid

    #102284

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting pfkB.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    pfkB gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge