We narrowed to 6,511 results for: itch
-
Plasmid#221922PurposeExpression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR7 (aa 2109-2434) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR7 region (aa 2109 - 2434); in…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR6-mCh-Cry2WT-NLS
Plasmid#221921PurposeExpression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR6 (aa 1856-2090) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR6 region (aa 1856-2090); inse…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR1-mCh-Cry2WT-NLS
Plasmid#221918PurposeExpression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR1 (aa 72-175) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR1 region (aa 72 - 175); inser…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-DmPGC
Plasmid#220309Purposered-light-activated guanylyl cyclase DmPGCDepositorInsertDmPGC
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
Plasmid#219653PurposeGeneral mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark VenusDepositorInsert2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
UseAAVTags2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)ExpressionMammalianMutationPromoternEf Addgene # 149296Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-lyn10-hLOV-ssrA-P2A-LOVPLD-mCherry-sspB
Plasmid#219557PurposePM-targeted iLID-LOVPLDDepositorInsertlyn10-iLID-LOVPLD
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ca-LiPPI-CLAPon1.0 (TEVp)
Plasmid#213409PurposeLight-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 2)DepositorInsertCaM-V5-TEVp
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
RGG-BcLOV-mCh-SOS
Plasmid#208278PurposeExpresses RGG-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.DepositorInsertRGG-BcLOV-mCh-SOS
UseLentiviralTagsExpressionMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Fus-BcLOV-mCh-EGFR
Plasmid#208281PurposeExpresses Fus-BcLOV-EGFR for optogenetic activation of EGFR signaling. Includes an mCherry tag.DepositorInsertFus-BcLOV-mCh-EGFR
UseLentiviralTagsExpressionMutationPromoterpCMVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC
Plasmid#202717PurposeMammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imagingDepositorArticleInsertsMyr-Pink Flamindo
EGFP-bPAC
UseLentiviralTags10xHis and mycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB EF1a CCR6-hLOV-TEVcs-Gal4
Plasmid#173120PurposeExpresses CCR6 hLOV receiver construct in DIPGDepositorInsertCCR6-hLOV-TEVcs-Gal4
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV CMV CCR6-eLOV-TEVcs-Gal4
Plasmid#173113PurposeExpresses CCR6 receiver construct in mammalian cellsDepositorInsertCCR6-eLOV-TEVcs-tTA
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-LexA-CIB1-biLINuS
Plasmid#192607PurposeLentiviral vector for constitutive expression of LexA-CIB1-biLINuSDepositorInsertLexA-CIB1-biLINuS
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-eGFP-P2A-CRY2-VPR
Plasmid#192610PurposeLentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR.DepositorInserteGFP-P2A-CRY2PHR-VPR
UseLentiviral and Synthetic BiologyTagseGFPExpressionMutationPromoterPGKAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (1)
Plasmid#185851PurposeTesting the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAP01
Plasmid#186260PurposesfGFP under light light responsive PEL222 promoterDepositorInsertsfGFP
UseTagsExpressionBacterialMutationPromoterPEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTA…Available sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
IBAR-Cry2PHR-WH2
Plasmid#176605PurposeCry2PHR with N-terminal IBAR domain and C-terminal WH2 domainDepositorInsertIBAR-Cry2PHR-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cry2PHR-IBAR-WH2
Plasmid#176603PurposeCry2PHR with C-terminal IBAR and WH2 domainsDepositorInsertCry2PHR-mCherry-IBAR-WH2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL30
Plasmid#174064PurposeEncodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.DepositorInsertpGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL15
Plasmid#174069PurposeExpresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC-lyn11-myc-LOV2GIVe (L)
Plasmid#170624PurposeFor the mammalian expression of mLOV2GIVe bearing the lit-mimicking mutation I539EDepositorInsertLyn11-myc-LOV2GIVe (L)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLIC-lyn11-myc-LOV2GIVe (D)
Plasmid#170625PurposeFor the mammalian expression of mLOV2GIVe dark-mimicking mutation C450SDepositorInsertLyn11-myc-LOV2GIVe (D)
UseTagsmycExpressionMammalianMutationPromoterCMVAvailable sinceJune 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD/His-D-iLight
Plasmid#170270PurposeExpression of iLight with 6xHis N-terminal tag in bacteria.DepositorInsertiLight
UseTags6xHis tagExpressionBacterialMutationSee CommentsPromoteraraBADAvailable sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_2pabPAC(Y72L)
Plasmid#158970PurposeA negative control for AAV-mediated 2P-activatable bPACDepositorInsert2pabPACY72L
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQP-2376
Plasmid#138974PurposeLight-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1DepositorInsertNcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
UseTagsExpressionMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLinkC-AI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMV
Plasmid#137115PurposeProtease Switch Clone ID: 1-A9DepositorInsertAI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFLinkC-AI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV
Plasmid#137116PurposeProtease Switch Clone ID: 1-C9DepositorInsertAI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK3b_007
Plasmid#123788PurposeEncodes bacterial light-activated adenylyl cyclase (bPAC) as a Type 3b part to be used in the MTK systemDepositorInsertbPAC
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_AtCRY2-PHR_X
Plasmid#131180PurposeExpresses AtCRY2-PHR (Arabidopsis thaliana) followed by restriction sitesDepositorInsertAtCRY2-PHR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0652
Plasmid#129013PurposeControl for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effectsDepositorInsert5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_EcSSPB-micro
Plasmid#131218PurposeExpresses EcSSPB-nano (Escherichia coli) preceded by restriction sitesDepositorInsertEcSSPB-micro
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_EcSSPB-nano
Plasmid#131219PurposeExpresses EcSSPB-micro (Escherichia coli) preceded by restriction sitesDepositorInsertEcSSPB-nano
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_EcSSPB-micro_X
Plasmid#131213PurposeExpresses EcSSPB-micro (Escherichia coli) followed by restriction sitesDepositorInsertEcSSPB-micro
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_AtCIB
Plasmid#131194PurposeExpresses AtCIB (Arabidopsis thaliana) preceded by restriction sitesDepositorInsertAtCIB
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_AtCRY2-PHR
Plasmid#131191PurposeExpresses AtCRY2-PHR (Arabidopsis thaliana) preceded by restriction sitesDepositorInsertAtCRY2-PHR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_RsLP-LOV
Plasmid#131188PurposeExpresses RsLP-LOV (Rhodobacter sphaeroides) preceded by restriction sitesDepositorInsertRsLP-LOV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_NcVVD-LOV
Plasmid#131187PurposeExpresses NcVVD-LOV (Neurosporra crassa) preceded by restriction sitesDepositorInsertNcVVD-LOV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_X_CrPH-LOV
Plasmid#131186PurposeExpresses CrPh-LOV domain (Chlamydomonas reinhardtii) preceded by restriction sitesDepositorInsertCrPH-LOV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_AtCIB_X
Plasmid#131181PurposeExpresses AtCIB (Arabidopsis thaliana) followed by restriction sitesDepositorInsertAtCIB
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOVC1_CrPH-LOV_X
Plasmid#131175PurposeExpresses CrPh-LOV domain (Chlamydomonas reinhardtii) followed by restriction sitesDepositorInsertCrPH-LOV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2019AvailabilityAcademic Institutions and Nonprofits only