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Addgene

We narrowed to 6,511 results for: itch

Showing: 5641 - 5680 of 6511 results
  1. pHR-IDR7-mCh-Cry2WT-NLS

    Plasmid
    #221922
    Purpose
    Expression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Insert
    CBP IDR7 (aa 2109-2434) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR7 region (aa 2109 - 2434); in…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pHR-IDR6-mCh-Cry2WT-NLS

    Plasmid
    #221921
    Purpose
    Expression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Insert
    CBP IDR6 (aa 1856-2090) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR6 region (aa 1856-2090); inse…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pHR-IDR1-mCh-Cry2WT-NLS

    Plasmid
    #221918
    Purpose
    Expression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WT
    Depositor
    Insert
    CBP IDR1 (aa 72-175) (CREBBP Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    insertion of the IDR1 region (aa 72 - 175); inser…
    Promoter
    Available since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)

    Plasmid
    #219653
    Purpose
    General mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark Venus
    Depositor
    Insert
    2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
    Use
    AAV
    Tags
    2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)
    Expression
    Mammalian
    Mutation
    Promoter
    nEf Addgene # 149296
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC

    Plasmid
    #202717
    Purpose
    Mammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imaging
    Depositor
    Inserts
    Myr-Pink Flamindo
    EGFP-bPAC
    Use
    Lentiviral
    Tags
    10xHis and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  6. pILGFP3A2 (1)

    Plasmid
    #185851
    Purpose
    Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter
    Depositor
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pJL30

    Plasmid
    #174064
    Purpose
    Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.
    Depositor
    Insert
    pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pGal1
    Available since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  8. pJL15

    Plasmid
    #174069
    Purpose
    Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.
    Depositor
    Insert
    pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
    Use
    Tags
    FLAG
    Expression
    Yeast
    Mutation
    Promoter
    pGal1
    Available since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pQP-2376

    Plasmid
    #138974
    Purpose
    Light-induced recruitment of NSImb-QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1
    Insert
    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NSImb-NES-mCh-Q-PAS1-NotI
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pMM0652

    Plasmid
    #129013
    Purpose
    Control for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) integration effects
    Depositor
    Insert
    5' URA3-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-Spacer-URA3 URA3 3'-KanR-ColE1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 5641 - 5680 of 6511 results