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pUC19-HRP-C

Plasmid

#40163

Depositor

Paul Ortiz de Montellano

Publication

Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

Insert

Horseradish peroxidase

Use

Coli cloning vector

Tags

Six histidine

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B EDD

Plasmid

#37188

Depositor

Darren Saunders, Charles Watts

Publication

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD (UBR5 Human)

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Please see Depositor Comments

Promoter

CMV

Available since

Sept. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pShuttle-CMV-iRFP

Plasmid

#31856

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Publication

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Use

Adenoviral

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Flag-HA-USP21

Plasmid

#22574

Depositor

Wade Harper

Publication

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP21 (USP21 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3.1/opto-a1AR-EYFP

Plasmid

#20947

Purpose

Mammalian expression of opto-a1AR fused to EYFP for optical control of GPCR signaling

Depositor

Karl Deisseroth

Publication

Airan et al Nature. 2009 Apr 23;458(7241):1025-9. do

Insert

opto-a1AR

Expression

Mammalian

Available since

May 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pKMyc

Plasmid

#19400

Depositor

Ian Macara

Publication

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Type

Empty backbone

Tags

Myc

Expression

Mammalian

Available since

April 2, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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YFP-tagged FRB (YR)

Plasmid

#20148

Depositor

Tobias Meyer

Publication

Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

Insert

YFP-FRB (YR)

Expression

Mammalian

Available since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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TetO-FUW-oct4

Plasmid

#20323

Depositor

Rudolf Jaenisch

Publication

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pRK5-myc-Rac1-wt

Plasmid

#12985

Depositor

Gary Bokoch

Insert

Rac1 (RAC1 Human)

Tags

Myc

Expression

Mammalian

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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anti-FLAG M2 heavy chain

Plasmid

#175359

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

Depositor

Joost Snijder

Publication

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 heavy chain

Tags

His8 tag and N-terminal secretion signal peptide …

Expression

Mammalian

Mutation

L51I (see paper)

Promoter

CMV

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDF0159 pCMV - huDisCas7-11 mammalian expression

Plasmid

#172507

Purpose

Encodes human codon optimized DisCas7-11 protein alone for expression in mammalian cells

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

pCMV - huDisCas7-11 mammalian expression

Expression

Mammalian

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pOpen_taq

Plasmid

#153315

Purpose

Bacterial expression of Taq polymerase

Depositor

Drew Endy

Insert

Taq polymerase

Expression

Bacterial

Promoter

Tac

Available since

June 2, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-FLEX-DTR-GFP

Plasmid

#124364

Purpose

conditional expression of a diphtheria toxin receptor (DTR)–GFP fusion protein

Depositor

Eiman Azim, Thomas Jessell

Publication

Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

Has service

AAV2

Insert

DTR-GFP

Use

AAV

Tags

GFP

Promoter

Chicken B-actin

Available since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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3xnls-mNeonGreen

Plasmid

#98875

Purpose

In vivo visualization of the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

3xNLS

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET-45b(+)-Tn5

Plasmid

#112112

Purpose

Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

Depositor

Frank Pugh

Publication

Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

Insert

Tn5

Tags

His6

Expression

Bacterial

Mutation

E54K E110K P242A L372P

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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SaBE4-Gam

Plasmid

#100809

Purpose

Expresses SaBE4-Gam in mammalian cells

Depositor

David Liu

Publication

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

SaBE4-Gam

Expression

Mammalian

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDH-CMV

Plasmid

#72265

Purpose

Express gene of interest under CMV promoter

Depositor

Kazuhiro Oka

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 25, 2016

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

56 Articles

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shh10

Plasmid

#64867

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

Depositor

John Flannery, David Schaffer

Publication

Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

Insert

shh10 cap

Use

AAV

Mutation

I319V, N451D, D532N

Available since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDestTol2pACryGFP

Plasmid

#64022

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving GFP in lens

Depositor

Joachim Berger, Peter Currie

Publication

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

GFP

Use

Gateway Cloning

Promoter

alpha-Acrystallin promoter

Available since

May 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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Tol2-elavl3-GCaMP6s

Plasmid

#59531

Purpose

Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Publication

Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
GCaMP6s

Available since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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