We narrowed to 15,661 results for: genome
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Plasmid#42248PurposegRNA targeted to zebrafish gene tia1lDepositorAvailable SinceJan. 31, 2013AvailabilityAcademic Institutions and Nonprofits only
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GTH-y
Plasmid#81105PurposeGene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…Available SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBCV
Plasmid#62659PurposeMammalian expression of FLPo recombinase from the broadly active CAG promoter/enhancerDepositorInsertFLPo
ExpressionMammalianAvailable SinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-AaCas12b(R911A)-2AeGFP
Plasmid#121961PurposeMammalian expression, Transcription regulation, deactivated AaCas12bDepositorInsertCatalytically deactivated AaCas12b
Tags3xHA tagExpressionMammalianMutationR911AAvailable SinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A0608
Plasmid#91018PurposeModule A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSPDepositorInsertCsy4-P2A-AtCas9_D10A nickase
UseCRISPRMutationD10APromoterEC1.2Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT112_GalL_nCDA1-xBE3
Plasmid#141318PurposeExpressing base editor nCDA1-xBE3 in yeast cellsDepositorInsertnCDA1-xBE3
UseCRISPRExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLS.546
Plasmid#184963PurposeTest effect of a1/a2 length on editing priB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
ExpressionBacterialMutationpriB donor G1069A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0121
Plasmid#177053PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoterDepositorInsertiridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0117
Plasmid#177049PurposeMoClo Level 1, position 6, transcriptional unit for transient expression of iridoid synthase (CrISY) from Catharanthus roseus driven by 35S promoterDepositorInsertiridoid synthase (CrISY) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL59-Nat
Plasmid#140465PurposeCRISPR/Cas9 engineering with nourseothricin selectionDepositorTypeEmpty backboneUseCRISPRExpressionBacterial and YeastAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A1710
Plasmid#91039PurposeModule A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9_H840A nickase, Terminator: HSPDepositorInsertCsy4-P2A-TaCas9_H840A nickase
UseCRISPRMutationH840APromoterZmUbiAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-SARA1
Plasmid#34809DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2519
Plasmid#91076PurposeModule B, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol IIIDepositorInsertEsp3I ccdb cassette for single gRNA spacer cloning
UseCRISPRPromoterOsU3Available SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR - Amplicon, BCR/ABL sgRNA 1
Plasmid#70659PurposeExpresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backboneDepositorInsertsgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLC-Flag-UBE2G1 sg2R-P2A-Hygro
Plasmid#124299PurposeLentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.DepositorInsertUBE2G1 (UBE2G1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationseveral silent mutations to disrupt sgRNA targeti…PromoterCMVAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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CROP_C9_Puro_FGFR1
Plasmid#183289PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR1 geneDepositorInsertFGFR1
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR2
Plasmid#183290PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR2 geneDepositorInsertFGFR2
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB2802
Plasmid#63655PurposepCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-HD118
Plasmid#172655PurposeExpressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 geneDepositorInsertpegRNA-HD118A/pegRNA-HD118B
UseCRISPRAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0108
Plasmid#177041PurposeMoClo Level 1, position 2, transcriptional unit for transient expression of geranyl pyrophosphate synthase (PaGPPS1) from Picea abies driven by 35S promoterDepositorInsertgeranyl pyrophosphate synthase (PaGPPS1) from Picea abies
UseSynthetic BiologyAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-D10A_5
Plasmid#91170PurposeCas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt IIDepositorInsertTaCas9_D10A (nickase)
UseCRISPRExpressionPlantMutationD10AAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLS.540
Plasmid#184959PurposeTest effect of a1/a2 length on editing fabH using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
ExpressionBacterialMutationfabH donor G954A, a1/a2 length extended to 22 bpPromoterT7/lacAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDest_pK7WG2_pRPS5A_CTP OleGFP
Plasmid#171723PurposeptpTALECD vector assembly, Destination vector;DepositorInsertRPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2
ExpressionPlantAvailable SinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
E2_pENTR_R4R3_T CTP
Plasmid#171736PurposeptpTALECD vector assembly, Entry clone 2 with attR4 and R3DepositorInsertA terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal
UseMultisite gateway entry vectorAvailable SinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB2990
Plasmid#63645PurposepCfB2990 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_EGFR
Plasmid#183285PurposeAll-in-One CRISPRko system with a guide RNA that targets EGFR geneDepositorInsertEGFR
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
PX458_RXRB_1
Plasmid#104050PurposeEncodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFPDepositorAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_SRC
Plasmid#183322PurposeAll-in-One CRISPRko system with a guide RNA that targets SRC geneDepositorInsertSRC
UseLentiviralAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ABL2
Plasmid#183268PurposeAll-in-One CRISPRko system with a guide RNA that targets ABL2 geneDepositorInsertABL2
UseLentiviralAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ABL1
Plasmid#183267PurposeAll-in-One CRISPRko system with a guide RNA that targets ABL1 geneDepositorInsertABL1
UseLentiviralAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCIP-hTRAC
Plasmid#154094PurposeSelf-Cutting and Integrating CRISPR backbone targeting human TCR-alpha common chainDepositorInsertsgRNA and BAIT targeting human TRAC locus
UseCRISPRExpressionMammalianAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUB6_EPB49
Plasmid#84015PurposeEPB49 minigene to test splicing eventDepositorAvailable SinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOG2003
Plasmid#49516PurposeTALEN backbone for expression of TALE(deltaRDV)-FokI RR in C. elegansDepositorTypeEmpty backboneTagsFokI-RR, HA, and SV40NLSExpressionWormPromoterhsp-16.2Available SinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_MBD1_iso1
Plasmid#86263PurposeDonor vector for 3' FLAG tag of human MBD1_iso1DepositorAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_ESR1
Plasmid#183288PurposeAll-in-One CRISPRko system with a guide RNA that targets ESR1 geneDepositorInsertESR1
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-H840A_6
Plasmid#91173PurposeCas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:barDepositorInsertTaCas9_H840A (nickase)
UseCRISPRExpressionPlantMutationH840AAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-H840A_1
Plasmid#91162PurposeCas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_H840A (nickase)
UseCRISPRExpressionPlantMutationH840AAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS316-RGR-GFP-mm
Plasmid#51059PurposeSame as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.DepositorInsertRGR-GFP
UseCRISPRExpressionBacterial and YeastPromoterYeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_TEAD1
Plasmid#86291PurposeDonor vector for 3' FLAG tag of human TEAD1DepositorAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only