We narrowed to 9,723 results for: SUB
-
Plasmid#115697Purposesuperfolder pHluorin for intracellular pH measurements in S. cerevisiae (improved performance when targeted to the ER or subsequent compartments)DepositorInsertsfpHluorin
ExpressionYeastMutationfor introduced mutations see publicationPromoterMET25Available SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSNAPf-Cox8A Control Plasmid
Plasmid#101129PurposeExpression of SNAP-Tagged Cox8A in Mammalian cellsDepositorAvailable SinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRL623
Plasmid#58494Purposehelper plasmid for bacterial conjugal DNA transferDepositorInsertencodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
Available SinceOct. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmax-SpG
Plasmid#211549PurposePrime editing in mammalian cellsDepositorInsertPEmax-SpG
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-DRGFP
Plasmid#113193PurposeDR-GFP substrate (Addgene #26475) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. DR-GFP cassette contains PGK-puromycin.DepositorInsertDR-GFP
UseDonor construct with t2a-hygromycin for gene trap…ExpressionMammalianAvailable SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-4mtD3cpv
Plasmid#36324DepositorInsert4mtD3cpv second generation cameleon (calcium sensor) targeted to mitochondria
TagsFirst 29 aa of human COX subunit 8aPromoterCMVAvailable SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Sec61β
Plasmid#172445PurposeExpression of ER protein Sec61beta with an N-terminal mCherry tagDepositorInsertProtein transport protein Sec61 subunit beta
TagsmCherryExpressionMammalianPromoterCMVAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pLVCT-tTR-KRAB
Plasmid#11643PurposeTet-regulated (Tet-on) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertCAG promoters, GFP, tTR-KRAB, Tet-on
UseLentiviralExpressionMammalianAvailable SinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMSP1D1-Strep-II
Plasmid#224209PurposeMembrane scaffold protein (MSP1D1) with Strep-II tag. pMSP1D1, 7xHis tag swapped for a Strep-II tag.DepositorInsertMSP1D1-Strep-II
TagsStrep-II TagExpressionBacterialMutationSubstitution of 7xHis tag found in pMSP1D1 for a …PromoterT7Available SinceJuly 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(C1)-PP1alpha
Plasmid#44224DepositorAvailable SinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLEXSY-hyg2.1_LtIFT43_Flag
Plasmid#194433PurposeExpression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolaeDepositorInsertIntraflagellar transport complex subunit 43
UseLeishmania tarentolae expressionTags3X Flag and LinkerPromoterL.donovani promoter elementsAvailable SinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS405-FlagPEN2/PS1wt
Plasmid#207803PurposeYeast integrative vector for expression of FlagPEN2 and Presenilin 1DepositorInsertGamma-secretase subunits PEN-2 and presenilin1 wt
ExpressionYeastAvailable SinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS402-nicastrin/APH-1a-HA
Plasmid#207804PurposeYeast integrative vector for expression of Nicastrin and APH-1a-HADepositorInsertGamma-secretase subunits nicastrin and Aph1a
ExpressionYeastAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S2
Plasmid#128590PurposeExpresses tagless S2 ribosomal protein in E.coliDepositorAvailable SinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-p47PX(aa2-132)
Plasmid#19007DepositorAvailable SinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
p40PX-EGFP
Plasmid#19010DepositorInsertp40phox (NCF4 Human)
TagsEGFPExpressionMammalianMutationaa1-148 (PX domains)PromoterCMVAvailable SinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLG015 (RADAR)
Plasmid#157893PurposeExpresses the RADAR defense systemDepositorInsertRADAR subtype containing auxillary gene rdrD
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-N488D
Plasmid#10988DepositorInsertrpb1-N488D (RPO21 Budding Yeast)
ExpressionBacterial and YeastMutationMutation 1462 A to G in the RPB1 ORF resulting in…Available SinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pACλCI-β-flap (831-1057)
Plasmid#53733PurposeExpression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertλCI-β-flap fusion
ExpressionBacterialPromoterlac UV5Available SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GFP-Sec61β
Plasmid#172442PurposeExpression of ER protein Sec61beta with an N-terminal FKBP-GFP tagDepositorInsertProtein transport protein Sec61 subunit beta
TagsFKBP-GFPExpressionMammalianPromoterCMVAvailable SinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(C1)-PP1gamma
Plasmid#44225DepositorAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3163
Plasmid#101248PurposeGFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.DepositorAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cherry-ICP0-C3
Plasmid#134560PurposeExpresses Cherry-ICP0 fusion proteinDepositorInsertICP0
TagsCherryExpressionMammalianPromoterCMVAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB1H2w2-zif268
Plasmid#18045DepositorInsertzif268
ExpressionBacterialMutationZif268 is fused to the omega subunit of RNA polym…Available SinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-tTR-KRAB
Plasmid#11642PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, tTR-KRAB, Tet-on
UseLentiviralExpressionMammalianAvailable SinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVET-tTR-KRAB
Plasmid#11644PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hEF-1alpha promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthEF-1alpha, GFP, tTR-KRAB, Tet-on
UseLentiviralExpressionMammalianAvailable SinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVCT-rtTR-KRAB-2SM2
Plasmid#11779PurposeTet-regulated (Tet-off) lentiviral vector for transgene (CAG promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertCAG promoters, GFP, rtTR-KRAB-2SM2, Tet-off
UseLentiviralExpressionMammalianAvailable SinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
HBV 1.3-mer P-null replicon
Plasmid#65462Purposecontaining 1.3 units of the P-null HBV genome (subtype ayw)DepositorInsert1.3 units of the P-null HBV genome
ExpressionMammalianAvailable SinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-FnCpf1
Plasmid#129554PurposeContains codon-optimized FnCpf1 and gRNA for streptomycesDepositorInsertFnCpf1
ExpressionBacterialPromoterrpsL(XC)-BbsIAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-2/Rabaptin-5:R5BD
Plasmid#168808PurposeProduction of recombinant GST-R5BD (Rab5-binding domain) in bacteria for GST pulldown assays to identify binding partners for Rab5 subfamily membersDepositorInsertRab5-binding domain (aa739-862) of Rabaptin-5
TagsGSTExpressionBacterialAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJB06
Plasmid#190480PurposeBase Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsCas9
XylR
UseE. coli / b. subtilis - c. difficile shuttle vect…PromoterxylR-driven promoterAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJB07
Plasmid#190481PurposeTargeting plasmid for 2-plasmid C. difficile mutagenesis systemDepositorInsertsUpstream & Downstream pyrE deletion region
gRNA
UseE. coli / b. subtilis - c. difficile shuttle vect…Available SinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-E1103G
Plasmid#10989DepositorInsertrpb1-E1103G (RPO21 Budding Yeast)
ExpressionBacterial and YeastMutationMutation 3308 A to G in the RPB1 ORF resulting in…Available SinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-rtTR-KRAB-2SM2
Plasmid#11652PurposeTet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, rtTR-KRAB-2SM2, Tet-off
UseLentiviralExpressionMammalianAvailable SinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAV0279
Plasmid#149688PurposeBacterial expression of SENP_Eu protease test substrate H14-SUMO_Eu1-MBP-3xFlagTagDepositorInsertSUMO_Eu1-MBP
Tags3xFLAG-tag and His14-tagExpressionBacterialAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR iSH-YFP-PIF
Plasmid#50841PurposeOptogenetic PI3K activation system; cytoplasmic componentDepositorInsertsp85 iSH2
PIF6 1-100
UseLentiviral and Synthetic BiologyAvailable SinceFeb. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET22b-csgA
Plasmid#89581PurposeExpress csgA curli protein with GS linker and HistaqDepositorAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCLIPf-Cox8A Control Plasmid
Plasmid#101130PurposeExpression of CLIP-Tagged Cox8A in Mammalian cellsDepositorAvailable SinceSept. 27, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVPRT-tTR-KRAB
Plasmid#11648PurposeTet-regulated (Tet-on) lentiviral vector for transgene (hPrion promoter) - OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInserthPrion, GFP, tTR-KRAB, Tet-on
UseLentiviralExpressionMammalianAvailable SinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET22b-csgB
Plasmid#89582PurposeExpress csgB curli protein with GS linker and HistaqDepositorAvailable SinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-p40PX(aa1-148)
Plasmid#19008DepositorAvailable SinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
p40PX-EYFP
Plasmid#19011DepositorAvailable SinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-rtTR-KRAB
Plasmid#11777PurposeTet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generationDepositorInsertmPGK, GFP, rtTR-KRAB, Tet-off
UseLentiviralExpressionMammalianAvailable SinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET16_Caspase-4_p10
Plasmid#214308PurposeProtein overexpression in bacteriaDepositorAvailable SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_Caspase-4_p20_C258A
Plasmid#214307PurposeProtein overexpression in bacteriaDepositorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
YFP-Rac2
Plasmid#11393DepositorAvailable SinceFeb. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEYFP(C1)-PP1alpha
Plasmid#44231DepositorAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5-SNAP-CLIP-GB1a
Plasmid#125709PurposeEukaryotic expression of a fusion protein encoding SNAP, CLIP and the GB1a subunit of the metabotropic GABA receptorDepositorInsertSNAP-CLIP-GB1a
ExpressionMammalianPromoterCMVAvailable SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mCherryGGBsMTS
Plasmid#133625PurposeExpression vector for mCherry fused to a membrane targeting sequence (MTS) of the B.subtilis MinD (C-terminal fusion) and an N-terminal His-tag for purification.DepositorInsertmCherryGGBsMTS
ExpressionBacterialAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only