We narrowed to 14,031 results for: TIM

1. 1/2-NORAD-(A)4x

   Plasmid

   #199208

   Purpose

   1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

   Depositor

   Esther Braselmann

   Article

   Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

   Insert

   1/2-NORAD
   

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   4 copies of Riboglow tag after stop codon

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-CaMKIIbeta(A303R)

   Plasmid

   #21224

   Depositor

   Tobias Meyer

   Article

   Shen et al Science. 1999 Apr 2. 284(5411):162-6.

   Insert

   CaMKIIbeta(A303R) (Camk2b Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   CaMKIIbeta(A303R) (Lab plasmid CF 0002)

   Available Since

   July 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV_Lyn11-mTurquoise2-iLID

   Plasmid

   #161000

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to lipidated peptide from LYN

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   Lyn11-mTurquoise2-iLID
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-AsCas12a-2AeGFP

   Plasmid

   #123638

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   AsCas12a
   

   Tags

   3xHA tag

   Expression

   Mammalian

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. HBT_pcoCASphi_version2

   Plasmid

   #197932

   Purpose

   Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pcoCasphi-2
   

   Use

   CRISPR

   Tags

   2xNLS-2xFLAG

   Expression

   Plant

   Promoter

   35SPPDK promoter

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS-H2B-EYFP

   Plasmid

   #53746

   Purpose

   contains fusion of human histone H2B to EYFP in pCS2+ vector.

   Depositor

   Sean Megason

   Article

   Megason et al Methods Mol Biol. 2009;546:317-32. doi:

   Insert

   histone H2B (H2BC21 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   simian CMV IE94

   Available Since

   July 8, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pLV_mTurquoise2-iLID-CAAX

   Plasmid

   #160999

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via C-terminal fusion to CAAX motif from KRAS

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   mTurquoise2-iLID-CAAX
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMito-Electra1

   Plasmid

   #179481

   Purpose

   Mitochondrial labeling with blue fluorescent protein Electra1 fused to partial COX8A

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   COX8A-Electra1
   

   Tags

   Electra1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYZ146

   Plasmid

   #98406

   Purpose

   Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Insert

   gRNA targeting Sp.leu1 (leu1 Fission Yeast)
   

   Use

   CRISPR

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS-H2B-citrine

    Plasmid

    #53752

    Purpose

    Contains fusion of human Histone H2B to citrine.

    Depositor

    Sean Megason

    Article

    Megason et al Methods Mol Biol. 2009;546:317-32. doi:

    Insert

    histone H2B (H2BC21 Human)
    

    Tags

    citrine

    Expression

    Mammalian

    Promoter

    simian CMV IE94

    Available Since

    July 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tom20-CIB-stop

    Plasmid

    #117243

    Purpose

    Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

    Depositor

    Robert Hughes

    Article

    Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

    Insert

    CIBN
    

    Expression

    Mammalian

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGrDL_SP

    Plasmid

    #83204

    Purpose

    receives miRNA target sequences for testing using the dual luciferase assay system

    Depositor

    Peer Schenk

    Article

    Moyle et al Front Plant Sci. 2017 Sep 20;8:1631. doi

    Insert

    SalI/PstI cloning sites
    

    Expression

    Plant

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-ChroME(S273A)-mRuby2-ST

    Plasmid

    #109128

    Purpose

    Modified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChroME(S273A)-mRuby2-ST
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mMaroon-RTK-mMaroon

    Plasmid

    #84359

    Purpose

    FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon

    Depositor

    Ryohei Yasuda

    Article

    Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    mMaroon-Rtk--mMaroon
    

    Tags

    mMaroon flanking Rtk domain on N and C terminus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEntr1a-wGBD-mTurquoise2-wGBD

    Plasmid

    #176136

    Purpose

    Visualization of active Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    WAS (WAS Human)
    

    Use

    Entry vector

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDM4-GLY

    Plasmid

    #49808

    Purpose

    Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration.

    Depositor

    Mattheos Koffas

    Article

    Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

    Inserts

    phosphoglycerate kinase
    glyceraldehyde-3-phosphate dehydrogenase
    pyruvate dehydrogenase subunit E1
    dihydrolipoamide acetyltransferase
    dihydrolipoamide dehydrogenase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. CeNL-Clathrin_pEGFP

    Plasmid

    #89540

    Purpose

    Cyan color variant of bright luminescent protein, fused to clathrin light chain

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    CeNL
    

    Tags

    Clathrin light chain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. YTIP-GFP

    Plasmid

    #42238

    Depositor

    William Pu

    Article

    von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

    Insert

    YTIP-GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. HaloTag-2xrGBD

    Plasmid

    #176109

    Purpose

    Visualization of active Rho

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    rhotekin (Rtkn Mouse)
    

    Expression

    Mammalian

    Promoter

    CMVdel

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYZ164

    Plasmid

    #98408

    Purpose

    Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

    Depositor

    Jef Boeke

    Article

    Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

    Insert

    gRNA targeting Sp.his3 (his3 Fission Yeast)
    

    Use

    CRISPR

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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