59,477 results

1. pPBbleo-EREG-ScNeo

   Plasmid

   #209905

   Purpose

   To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   EREG-ScNeo (EREG Human)
   

   Tags

   GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

   Expression

   Mammalian

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF2-EFGR-hMer

   Plasmid

   #225996

   Purpose

   Expresses a chimeric construct having N-terminal human EGFR and C-terminal human MerTK

   Depositor

   Raymond Birge, Sergei Kotenko

   Article

   Kimani et al Cell Commun Signal. 2016 Sep 6;14(1):19.

   Insert

   human EGFR N-Terminal human MerTK C-Terminal Fusion (EGFR, MERTK Human)
   

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Nov. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-PARP1-del.p119K120S–eGFP

   Plasmid

   #211554

   Purpose

   A piggyBac vector containing CMV-PARP1-del.p119K120S-eGFP-IRES-Neo cassette.

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Piggybac

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   deletion of amino acids 119K 120S

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1-PARP1-delM43;F44I

   Plasmid

   #211579

   Purpose

   CMV driven PARP1-delM43;F44I -eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Contains deletion of M43 and F44I

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CHFR-PBZ-eGFP

   Plasmid

   #211581

   Purpose

   Expressing CHFR PBZ domain fused to eGFP

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   CHFR PBZ domain (CHFR Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   PBZ domain of the protein

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-OSF-ATG13 (2-197)

   Plasmid

   #188075

   Purpose

   To make ATG101/ATG13 HORMA complex for expression in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG13 (ATG13 Human)
   

   Tags

   strep-strep-Flag tag

   Expression

   Mammalian

   Mutation

   ATG13 (2-197)

   Promoter

   CMV

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH6HTC_CCDC124-Halo

   Plasmid

   #175328

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   CCDC124 (CCDC124 Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. PDZK1_PDZ_2

   Plasmid

   #103943

   Purpose

   Protein expression and purification of PDZK1 PDZ2 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PDZK1 (PDZK1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. PDZK1_PDZ_3

   Plasmid

   #103944

   Purpose

   Protein expression and purification of PDZK1 PDZ3 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PDZK1 (PDZK1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac-ATG101-ATG13 HORMA

    Plasmid

    #99332

    Purpose

    Mammalian expression of one subcomplex from the Human ULK1 complex

    Depositor

    James Hurley

    Article

    Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

    Inserts

    ATG101 (ATG101 Human)
    ATG13 (ATG13 Human)
    

    Tags

    GST

    Expression

    Insect

    Promoter

    p10 and pH

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. CL20_mEGFP_HEY1_NCOA2

    Plasmid

    #205829

    Purpose

    Express mEGFP-tagged fusion protein, HEY1_NCOA2 from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    HEY1_NCOA2 (HEY1, NCOA2 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pVax1-hVEGF165

    Plasmid

    #74466

    Purpose

    expression of hVEGF165 in mammalian cells

    Depositor

    Loree Heller

    Article

    Marshall WG et al Gene Ther. 2010 Mar;17(3):419-23. doi: 1

    Insert

    vascular endothelial growth factor 165 (VEGFA Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. dTAG NLRP1

    Plasmid

    #166825

    Purpose

    dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-N1-TFE3

    Plasmid

    #38120

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

    Insert

    TFE3 (TFE3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted stop codon

    Promoter

    CMV

    Available Since

    July 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. P532_POU4F2-p2A-tdTomato-p2A-Thy1.2

    Plasmid

    #239102

    Purpose

    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of POU4F2 (aka BRN3B). This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

    Depositor

    Karl Wahlin

    Article

    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

    Insert

    POU4F2 (POU4F2 Human)
    

    Use

    Donor plasmid for integration into the human geno…

    Tags

    p2A-tdTomato-p2A-Thy1.2

    Expression

    Mammalian

    Promoter

    Endogenous POU4F2

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-eSpCas9-2A-GFP-sgRNA-DNA-PKcs

    Plasmid

    #220493

    Purpose

    To generate DNA-PKcs KO in human cells. Co-expresses eSpCas9(1.1), GFP and a guide RNA against DNA-PKcs exon 1.

    Depositor

    Sébastien Britton

    Article

    Bossaert et al bioRxiv [Preprint]. 2024 Jan 4:2024.01.0

    Insert

    sgRNA targeting DNA-PKcs (PRKDC) exon 1 (PRKDC Human)
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Arf4-GFP

    Plasmid

    #39556

    Depositor

    Paul Melancon

    Article

    Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

    Insert

    ADP ribosylaion factor 4 (ARF4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB-TAG-NICD

    Plasmid

    #130934

    Purpose

    PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

    Depositor

    James Thomson

    Article

    Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

    Insert

    NICD (NOTCH1 Human)
    

    Expression

    Mammalian

    Promoter

    TetO

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR223-PDGFRA

    Plasmid

    #23892

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PDGFRA (PDGFRA Human)
    

    Use

    Gateway donor vector

    Mutation

    M260I

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK290 (mAID-mClover-Hygro)

    Plasmid

    #72828

    Purpose

    C-terminal tagging with mAID-mClover using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-mClover-Hygro
    

    Use

    CRISPR

    Tags

    mAID-mClover

    Expression

    Mammalian

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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