We narrowed to 30,451 results for: Ide

1. SAM-DNMT3A empty

   Plasmid

   #213164

   Purpose

   Empty vector (no sgRNA) for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   E1Fa and U6

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SAM-DNMT3A+sgGFP

   Plasmid

   #213165

   Purpose

   Vector with sgGFP for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgGFP
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   E1Fa and U6 and PGK

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SAM-DNMT3A+sgAAVS1_145

   Plasmid

   #213166

   Purpose

   Vector with sgAAVS1 for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgAAVS1_145
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   E1Fa and U6

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SAM-DNMT3A+sgAAVS1_118

   Plasmid

   #213167

   Purpose

   Vector with sgAAVS1 for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Insert

   sgAAVS1_118
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   E1Fa and U6

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SAM-DNMT3A-inactiveEmpty

   Plasmid

   #213168

   Purpose

   Empty Vector (no sgRNA) used as a negative control (inactive DNMT3A) for induction of global DNA methylation.

   Depositor

   Sefi Rosenbluh

   Article

   Hosseinpour et al Nat Commun. 2024 Dec 1;15(1):10449. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   E1Fa and U6

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPICZ A-SLC25A46[I349D]-His (SB256)

   Plasmid

   #227605

   Purpose

   Inducible expression of His-tagged SLC25A46 with I349D mutation in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Tags

   10xHis

   Expression

   Yeast

   Mutation

   I349D

   Promoter

   AOX1

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-SLC25A46[R257Q]-P2A-Electra1 (pAH11)

   Plasmid

   #227616

   Purpose

   Overexpression of SLC25A46 with R257Q mutation in mammalian cell lines

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Tags

   P2A-mElectra1

   Expression

   Mammalian

   Mutation

   R257Q

   Promoter

   CMV, T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-SLC25A46[R340C]-P2A-Electra1 (pAH12)

   Plasmid

   #227617

   Purpose

   Overexpression of SLC25A46 with R340C mutation in mammalian cell lines

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Tags

   P2A-mElectra1

   Expression

   Mammalian

   Mutation

   R340C

   Promoter

   CMV, T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-SLC25A46[I349D]-P2A-Electra1 (pAH13)

   Plasmid

   #227618

   Purpose

   Overexpression of SLC25A46 with I349D mutation in mammalian cell lines

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Tags

   P2A-mElectra1

   Expression

   Mammalian

   Mutation

   I349D

   Promoter

   CMV, T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

    Plasmid

    #227619

    Purpose

    Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    deleted aa 2-83

    Promoter

    CMV, T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKT-CNP

    Plasmid

    #211803

    Purpose

    Expresses 2',3'-cyclic nucleotide phosphodiesterase catalytic domain

    Depositor

    Emily Weinert

    Article

    Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

    Insert

    2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)
    

    Expression

    Bacterial

    Promoter

    tet

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ku70 insUP+pLDP1+NAT+tNOS+ku70 insD (SBE193)

    Plasmid

    #195054

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pLDP1 for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Expression

    Bacterial

    Promoter

    pLDP1

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. ku70 insUP+pFBA+NAT+tNOS+ku70 insD (SBE179)

    Plasmid

    #195053

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pFBA for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Expression

    Bacterial

    Promoter

    pFBA

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. ku70 insUP+pXYL+NAT+tNOS+ku70 insD (SBE178)

    Plasmid

    #195052

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pXYL for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Expression

    Bacterial

    Promoter

    pXYL

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. ku70 insUP+pGPD+NAT+tNOS+ku70 insD (SBE175)

    Plasmid

    #195051

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pGPD for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Expression

    Bacterial

    Promoter

    pGPD

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. ku70 insUP+pADH2+NAT+tNOS+ku70 insD (SBE174)

    Plasmid

    #195050

    Purpose

    integration cassette for ku70 deletion and expression of NAT gene under pADH2 for characterization

    Depositor

    Petri-Jaan Lahtvee

    Article

    Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

    Insert

    NAT
    

    Expression

    Bacterial

    Promoter

    pADH2

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. rUBR5_2383-2442_pGEX6P1

    Plasmid

    #209791

    Purpose

    Bacterial expression of UBR5 Mlle domain

    Depositor

    Kalle Gehring

    Article

    Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

    Insert

    Mlle domain of UBR5 (Ubr5 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 2383-2442 only

    Promoter

    tac

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-HypaR-SpCas9-NLS-6xHis

    Plasmid

    #207387

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    HypaR-SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R661A, N692A, M694A, Q695A, H698A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PET-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207382

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. B-HiFi SpCas9

    Plasmid

    #207362

    Purpose

    Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    CBh

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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