We narrowed to 4,337 results for: U6 gRNA

1. HypaR-SpCas9

   Plasmid

   #126757

   Purpose

   Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A, N692A, M694A, Q695A, H698A

   Promoter

   CBh

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXPR_220

   Plasmid

   #164560

   Purpose

   Lentiviral vector that encodes two sgRNA expression cassettes (one constitutive, one inducible via Flp). Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

   Inserts

   First sgRNA cassette
   Second sgRNA cassette
   

   Use

   CRISPR and Lentiviral; Flp/frt

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6 and Mouse U6

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. B-HypaR-SpCas9

   Plasmid

   #126764

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Hypa2SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R661A; N692A; M694A; Q695A; H698A; amino acids 10…

   Promoter

   CBh

   Available since

   Sept. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-As-crRNA

   Plasmid

   #78956

   Purpose

   Cloning vector for expression of AsCpf1 crRNA. It contains BsmB1 site for cloning.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available since

   June 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJW1219

   Plasmid

   #61250

   Purpose

   CRISPR/Cas9 plasmid containing sgRNA(F+E); for use in C. elegans

   Depositor

   Jordan Ward

   Article

   Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

   Insert

   sgRNA(F+E)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   U6 promoter

   Available since

   Dec. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMA3735

   Plasmid

   #184848

   Purpose

   gRNA cloning using blue-white screening for recombinants

   Depositor

   Mikhail Alexeyev

   Article

   Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

   Insert

   LacZalpha
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   alpha-fragment of LacZ

   Promoter

   U6

   Available since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC1817_pCR8-gSMN2-EX

   Plasmid

   #118651

   Purpose

   Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   gSMN2-EX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMB70

   Plasmid

   #47943

   Purpose

   Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

   Depositor

   Mike Boxem

   Article

   Waaijers et al Genetics. 2013 Aug 26.

   Insert

   guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Oct. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAC1816_pCR8-gSMN2-DN4

   Plasmid

   #118649

   Purpose

   Transient transfection; Expressed SMN2-DN4 CasRx gRNA; Gateway Donor

   Depositor

   Albert Cheng

   Article

   Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

   Insert

   gSMN2-DN4
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. sg_shuttle_RFP657

    Plasmid

    #134968

    Purpose

    Lentiviral vector for sgRNA expression

    Depositor

    Beat Bornhauser

    Article

    Huang et al Hemasphere. 2020 Sep 30;4(5):e456. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6-sgRNA; EFS-RFP657

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC1813_pCR8-gSMN2-DN1

    Plasmid

    #118646

    Purpose

    Transient transfection; Expressed SMN2-DN1 CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-DN1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAC1814_pCR8-gSMN2-DN2

    Plasmid

    #118647

    Purpose

    Transient transfection; Expressed SMN2-DN2 CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-DN2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAC1815_pCR8-gSMN2-DN3

    Plasmid

    #118648

    Purpose

    Transient transfection; Expressed SMN2-DN3 CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-DN3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSuper-Rem2-1

    Plasmid

    #51584

    Purpose

    shRNA 1 against rodent Rem2

    Depositor

    Suzanne Paradis

    Article

    Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

    Insert

    Rem2 (Rem2, Rem2 Rat, Mouse)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pAC1818_pCR8-gRG6-SA

    Plasmid

    #118652

    Purpose

    Transient transfection; Expressed RG6-SA CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gRG6-SA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAV hTERTsh

    Plasmid

    #111380

    Purpose

    Knock down of hTERT transcript

    Depositor

    Klaus Holzmann

    Article

    Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

    Insert

    shRNA C3 against hTERT
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

    Plasmid

    #91879

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

18. AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

    Plasmid

    #91880

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

19. px458-AMBRA1 (optimized for N-terminal knock-in)

    Plasmid

    #172608

    Purpose

    Expresses a gRNA against AMBRA1 and Cas9 from S. pyogenes with 2A-EGFP. This gRNA is suitable for the N-terminal knock-in of AMBRA1.

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    gRNA against AMBRA1 (AMBRA1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

    Plasmid

    #91884

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Gfi1 (Gfi1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only