We narrowed to 23,099 results for: crispr

1. pKO025

   Plasmid

   #242913

   Purpose

   aTc-inducible expression of Spy-dCas9-AsiA and Sth1-dCas9, and constitutive expression of MCP-SoxS

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

   Insert

   Sth1-dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Ptet

   Available Since

   Oct. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLP16_Lenti Scramble Control

   Plasmid

   #239417

   Purpose

   Negative control Lentiviral plasmid for SpCas9-based CRISPR KO

   Depositor

   Subhojit Roy

   Article

   Parra-Rivas et al STAR Protoc. 2025 Jul 21;6(3):103945. do

   Insert

   Scramble sequence
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCK320

   Plasmid

   #242906

   Purpose

   aTc-inducible expression of dCas9-AsiA

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

   Insert

   AsiA (asiA Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   TetR-Ptet

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCK321

   Plasmid

   #242912

   Purpose

   aTc-inducible expression of dCas9-AsiA and constitutive expression of MCP-SoxS

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

   Insert

   MCP-SoxS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   BBa_J23107

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNS1D-sgRNA9-SpR

   Plasmid

   #191092

   Purpose

   aTc inducible gRNA expression plasmid with BbsI sites for Golden Gate insertion of crRNA oligos and homology arms for genome integration at NS1D in Synechococcus sp. PCC 7002

   Depositor

   Anne Ruffing

   Article

   Dallo et al ACS Synth Biol. 2023 Apr 21;12(4):1175-1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tet

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MIGR1-U6gRNA1-Filler-v1

   Plasmid

   #237399

   Purpose

   For inserting a single guide RNA or later cloning two U6-gRNA cassettes with MIGR1-U6gRNA1-Filler-v2.

   Depositor

   Zuoming Sun

   Article

   Zhong et al STAR Protoc. 2025 Jun 20;6(2):103831. do

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, PGK

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MIGR1-U6gRNA1-Filler-v2

   Plasmid

   #237400

   Purpose

   For inserting a single guide RNA or later cloning two U6-gRNA cassettes with MIGR1-U6gRNA1-Filler-v1.

   Depositor

   Zuoming Sun

   Article

   Zhong et al STAR Protoc. 2025 Jun 20;6(2):103831. do

   Type

   Empty backbone

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, PGK

   Available Since

   June 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMEDS

   Plasmid

   #236568

   Purpose

   Introduces a multiple expression and dual selection (MEDS) system to use alongside CRISPR for applications such as gene editing.

   Depositor

   Shannon Moonah

   Article

   Sharma et al PLoS One. 2025 May 6;20(5):e0321881. doi

   Inserts

   CD/UPRT (negative selection)
   NPT II (positive selection)
   

   Use

   Tags

   3x Myc and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Reckleen_3plus_sgRNA_ptet_pos4

   Plasmid

   #233459

   Purpose

   Level 1 part, Ptet sfgfp dropout sgRNA for position 4, for building RECKLEEN plasmid with multiple sgRNAs

   Depositor

   Anke Becker

   Article

   Elsayed et al Research Square (2025) [Preprint]. doi:

   Insert

   Level 1 part, Ptet sfgfp dropout sgRNA for position 4, ColE1 ori, and KanR.
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 24, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. Reckleen_3plus_sgRNA_ptet_pos5

    Plasmid

    #233460

    Purpose

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5, for building RECKLEEN plasmid with multiple sgRNAs

    Depositor

    Anke Becker

    Article

    Elsayed et al Research Square (2025) [Preprint]. doi:

    Insert

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5, ColE1 ori, and KanR.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. Reckleen_3plus_sgRNA_ptet_pos5a

    Plasmid

    #233461

    Purpose

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, for building RECKLEEN plasmid with multiple sgRNAs

    Depositor

    Anke Becker

    Article

    Elsayed et al Research Square (2025) [Preprint]. doi:

    Insert

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5a, ColE1 ori, and KanR.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. Reckleen_3plus_sgRNA_ptet_pos5a1

    Plasmid

    #233462

    Purpose

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, for building RECKLEEN plasmid with multiple sgRNAs

    Depositor

    Anke Becker

    Article

    Elsayed et al Research Square (2025) [Preprint]. doi:

    Insert

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5a1, ColE1 ori, and KanR.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. Reckleen_3plus_sgRNA_ptet_pos5a2

    Plasmid

    #233463

    Purpose

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5a2, for building RECKLEEN plasmid with multiple sgRNAs

    Depositor

    Anke Becker

    Article

    Elsayed et al Research Square (2025) [Preprint]. doi:

    Insert

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5a2, ColE1 ori, and KanR.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. Reckleen_3plus_sgRNA_ptet_pos5b

    Plasmid

    #233464

    Purpose

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, for building RECKLEEN plasmid with multiple sgRNAs

    Depositor

    Anke Becker

    Article

    Elsayed et al Research Square (2025) [Preprint]. doi:

    Insert

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5b, ColE1 ori, and KanR.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. Reckleen_3plus_sgRNA_ptet_pos5b1

    Plasmid

    #233465

    Purpose

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5b1, for building RECKLEEN plasmid with multiple sgRNAs

    Depositor

    Anke Becker

    Article

    Elsayed et al Research Square (2025) [Preprint]. doi:

    Insert

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5b1, ColE1 ori, and KanR.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. Reckleen_3plus_sgRNA_ptet_pos5b2

    Plasmid

    #233466

    Purpose

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5b2, for building RECKLEEN plasmid with multiple sgRNAs

    Depositor

    Anke Becker

    Article

    Elsayed et al Research Square (2025) [Preprint]. doi:

    Insert

    Level 1 part, Ptet sfgfp dropout sgRNA for position 5b2, ColE1 ori, and KanR.
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. Reckleen_1_Cas9

    Plasmid

    #233456

    Purpose

    Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

    Depositor

    Anke Becker

    Article

    Elsayed et al Research Square (2025) [Preprint]. doi:

    Insert

    Level 2 RECKLEEN plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Reckleen_2_SpGCas9

    Plasmid

    #233457

    Purpose

    Level 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniae

    Depositor

    Anke Becker

    Article

    Elsayed et al Research Square (2025) [Preprint]. doi:

    Insert

    Level 2 RECKLEEN plasmid
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CL7_2xNLS_iGeoCas9(C2)_2xNLS_His

    Plasmid

    #222988

    Purpose

    Bacterial expression of iGeoCas9(C2) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C2)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX458-PLK4

    Plasmid

    #227310

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of PLK4 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PLK4 (PLK4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pUt-MONARCH 2.0_crEGFP

    Plasmid

    #224793

    Purpose

    LPUtopia matching RMCE donor plasmid with MONARCH 2.0 circuit with crRNA targeting EGFP.

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Inserts

    crEGFP
    Triplex stablier
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-d2i

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUC-hU6-2O-crScaffold_SV40-BFP

    Plasmid

    #224861

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pUC-hU6-2O-crScaffold_EF1a-BFP

    Plasmid

    #224862

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6-2xTetO

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CL7_2xNLS_iGeoCas9(C1)_2xNLS_His

    Plasmid

    #222987

    Purpose

    Bacterial expression of iGeoCas9(C1) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
    

    Use

    CRISPR

    Tags

    CL7 and His

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    July 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGGC-LexACasPea

    Plasmid

    #210539

    Purpose

    Cloning estradiol-inducible expression cassette containi LexA XVE binding sequence followed by SpCas9 CDS and PEA3AT as GreenGate module C

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    LexA::SpCas9::PEA3AT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCAG-PlmCasX-T2A-EGFP

    Plasmid

    #188273

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    PlmCasX
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCK411.RR1

    Plasmid

    #192644

    Purpose

    Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Insert

    BBa_J23119-RR1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23119

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHAGE-IRES-puro-NLS-dPbaCas13b-EGFP-NLS

    Plasmid

    #191365

    Purpose

    Overexpression of NLS-dPbaCas13b-EGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dPbaCas13b-EGFP-NLS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pHAGE-IRES-puro-NLS-dPba5Cas13b-EGFP-NLS

    Plasmid

    #191364

    Purpose

    Overexpression of NLS-dPba5Cas13b-EGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dPba5Cas13b-EGFP-NLS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHAGE-IRES-puro-NLS-dPba4Cas13b-EGFP-NLS

    Plasmid

    #191363

    Purpose

    Overexpression of NLS-dPba4Cas13b-EGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dPba4Cas13b-EGFP-NLS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pHAGE-IRES-puro-NLS-dHgm6Cas13b-EGFP-NLS

    Plasmid

    #191366

    Purpose

    Overexpression of NLS-dHgm6Cas13b-EGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dHgm6Cas13b-EGFP-NLS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHAGE-IRES-puro-NLS-dBba2Cas13b-EGFP-NLS

    Plasmid

    #191362

    Purpose

    Overexpression of NLS-dBba2Cas13b-EGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dBba2Cas13b-EGFP-NLS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pHAGE-IRES-puro-NLS-dPba3Cas13b-EGFP-NLS

    Plasmid

    #191361

    Purpose

    Overexpression of NLS-dPba3Cas13b-EGFP-NLS in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    NLS-dPba3Cas13b-EGFP-NLS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. DpbdCas12e KRAB

    Plasmid

    #188505

    Purpose

    Expresses FLAG tagged DpbdCas12e KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D672A/E769A/D935A

    Promoter

    EF1a

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPBO.ACT004

    Plasmid

    #182714

    Purpose

    Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region

    Depositor

    Joseph Schoeniger

    Article

    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

    Insert

    CasRx crRNA cloning backbone
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Catalytically deactivated R295A, H300A, R849A, H8…

    Promoter

    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

    Available Since

    Aug. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDIV492

    Plasmid

    #177700

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pOC4 Grin1-smFP-V5 KI

    Plasmid

    #183439

    Purpose

    FlpON knock-in for GluN1-smFP-V5 (amino acid position: A20)

    Depositor

    Harold MacGillavry

    Article

    Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

    Insert

    gRNA and smFP V5 donor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pX459-sg-tdTomato166/892

    Plasmid

    #179919

    Purpose

    Double cutting Cas9 plasmid with puromycin resistance and a single guide RNA targeting tdTomato fluorescent protein sites 166 and 892.

    Depositor

    Indeever Madireddy, Johan Sosa

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDIV491

    Plasmid

    #177699

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDIV488

    Plasmid

    #177696

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…

    Available Since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pPPC018.AAV

    Plasmid

    #171146

    Purpose

    Expression of J1-BBa_J23117-mRFP and hAAVS1 scRNA on pRK2-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    hAAVS1 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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42. pPPC016.109

    Plasmid

    #171144

    Purpose

    Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J109 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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43. pBbdCas9S_P0526-sgRNArodA

    Plasmid

    #149658

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, P0526

    Available Since

    Sept. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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44. pMiniT:myc-GluA2 TKIT donor

    Plasmid

    #169423

    Purpose

    Donor DNA for TKIT knockin of Myc to GluA2 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Myc (Myc Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

    Plasmid

    #149346

    Purpose

    Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Cbc

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pCbh-SpyCas9-T2A-puro-pA_U6-sgPolb (pE047)

    Plasmid

    #149350

    Purpose

    Mammalian expression vector for SpyCas9, puromycin resistance and human Polb guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    SpyCas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. pBbdCas9S_PflaBS-sgRNAflaB

    Plasmid

    #149643

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, PflaBS

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pBbdCas9S_P0826L-sgRNAflaB

    Plasmid

    #149648

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, P0826L

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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49. pBbdCas9S_Psyn-sgRNArodA

    Plasmid

    #149655

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi rodA

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNArodA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pBbdCas9S_PresTS-sgRNAflaB

    Plasmid

    #149644

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi flaB

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAflaB
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, PresTS

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge