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We narrowed to 12,028 results for: LEA

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Showing: 2921 - 2940 of 12028 results

  1. pGBW-m4046747

    Plasmid
    #149249
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149249-plasmid-map-sequence-id-358332

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  2. pGBW-m4046799

    Plasmid
    #145577
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145577-plasmid-map-sequence-id-292504

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  3. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS

    Plasmid
    #134653
    Purpose
    lysosome-targeted Sac1 catalytic domain C389S
    Depositor
    Roberto Zoncu
    Article
    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218
    Insert
    Sac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
    Use
    Lentiviral
    Tags
    FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…
    Expression
    Mammalian
    Mutation
    C389S and contains only amino acids 77-520 (Pleas…
    Available Since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    134653-plasmid-map-sequence-id-266494

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  4. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid
    #122085
    Purpose
    Express M.EcoGII fused to CENPC with destabilization domain - inducible
    Depositor
    Laure Crabbe
    Article
    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5
    Insert
    DD-linker-M.EcoGII-V5-CENPC (CENPC Human, E.coli)
    Use
    Retroviral
    Tags
    V5 tag on Nter of CENPC
    Expression
    Bacterial and Mammalian
    Mutation
    L341F, R930L (see comments below)
    Available Since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    122085-plasmid-map-sequence-id-238127

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  7. pGEX6P1-N-Strep2

    Plasmid
    #119758
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Promoter
    tac promoter
    Available Since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119758-plasmid-map-sequence-id-233666

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  20. EC71174

    Plasmid
    #154067
    Purpose
    Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Inserts
    HS Cre recombinase
    Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
    HYGROMYCIN Resistance Cassette
    Use
    Cre/Lox and Synthetic Biology; Golden gate
    Expression
    Bacterial and Plant
    Mutation
    The Arabidopsis thaliana U5 small nuclear ribonuc…
    Promoter
    35S promoter, Hordeum vulgare HSP 17 promoter use…
    Available Since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154067-plasmid-map-sequence-id-313091

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Showing: 2921 - 2940 of 12028 results