We narrowed to 14,345 results for: TIM

Showing: 2961 - 2980 of 14345 results

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12URA-B-ScTop2∆CTD

Plasmid

#214924

Purpose

Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

Depositor

James Berger

Article

Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

Insert

topoisomerase II

Tags

6x His-TEV

Expression

Yeast

Mutation

Deleted amino acids 1178-1428

Promoter

Gal 1/10

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pYZ146

Plasmid

#98406

Purpose

Cas9-gRNA leu1 plasmid for leu1 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.leu1 (leu1 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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HBT_pcoCASphi_version2

Plasmid

#197932

Purpose

Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Insert

pcoCasphi-2

Use

CRISPR

Tags

2xNLS-2xFLAG

Expression

Plant

Promoter

35SPPDK promoter

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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1/8-NORAD-(A)4x

Plasmid

#199209

Purpose

1/8 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

Depositor

Esther Braselmann

Article

Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

Insert

1/8-NORAD

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

4 copies of Riboglow tag after stop codon

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pMito-Electra1

Plasmid

#179481

Purpose

Mitochondrial labeling with blue fluorescent protein Electra1 fused to partial COX8A

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

COX8A-Electra1

Tags

Electra1

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-H3.3-YFP

Plasmid

#8693

Depositor

Kami Ahmad

Article

Janicki et al Cell 2004 Mar 5;116(5):683-98.

Insert

H3.3A (His3.3A Fly)

Tags

YFP

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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1/2-NORAD-(A)4x

Plasmid

#199208

Purpose

1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

Depositor

Esther Braselmann

Article

Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

Insert

1/2-NORAD

Tags

Riboglow RNA tag

Expression

Mammalian

Mutation

4 copies of Riboglow tag after stop codon

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pUCBB-ctH6-eGFP

Plasmid

#32562

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Promoter

Consitutive lac

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pEntr1a-wGBD-mTurquoise2-wGBD

Plasmid

#176136

Purpose

Visualization of active Cdc42

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

WAS (WAS Human)

Use

Entry vector

Available Since

Oct. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS010: CheRiff-eGFP channelrhodopsin (plasma membrane)

Plasmid

#163044

Purpose

CheRiff-eGFP channelrhodopsin (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

CheRiff

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-C1V1-TT-mRuby2-ST

Plasmid

#109124

Purpose

Cation channelrhodopsin C1V1 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

C1V1-TT-mRuby2-ST

Expression

Mammalian

Promoter

CAG

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCS-H2B-citrine

Plasmid

#53752

Purpose

Contains fusion of human Histone H2B to citrine.

Depositor

Sean Megason

Article

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Insert

histone H2B (H2BC21 Human)

Tags

citrine

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

July 8, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-GFP-2A-oG-WPRE

Plasmid

#102984

Purpose

Lentivirus expressing eGFP and optimised Rabies Glycoprotein (oG)

Depositor

Marco Tripodi

Article

Ciabatti et al Cell. 2017 Jul 1. pii: S0092-8674(17)306

Inserts

Enhanced Green Fluorescent Protein (eGFP)
Optimised Rabies glycoprotein (oG)

Use

Lentiviral

Available Since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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mMaroon-RTK-mMaroon

Plasmid

#84359

Purpose

FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mMaroon-Rtk--mMaroon

Tags

mMaroon flanking Rtk domain on N and C terminus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRSETA-qmTQ2-citrate

Plasmid

#225251

Purpose

Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

Depositor

Yasushi Okada

Article

Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

Insert

qmTQ2-citrate

Tags

6XHis, T7 tag, XpressTM tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pYZ164

Plasmid

#98408

Purpose

Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.his3 (his3 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGrDL_SP

Plasmid

#83204

Purpose

receives miRNA target sequences for testing using the dual luciferase assay system

Depositor

Peer Schenk

Article

Moyle et al Front Plant Sci. 2017 Sep 20;8:1631. doi

Insert

SalI/PstI cloning sites

Expression

Plant

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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HaloTag-2xrGBD

Plasmid

#176109

Purpose

Visualization of active Rho

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

rhotekin (Rtkn Mouse)

Expression

Mammalian

Promoter

CMVdel

Available Since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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CeNL-Clathrin_pEGFP

Plasmid

#89540

Purpose

Cyan color variant of bright luminescent protein, fused to clathrin light chain

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

CeNL

Tags

Clathrin light chain

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMX Vav1 uGFP

Plasmid

#14557

Depositor

Joan Brugge

Article

Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

Insert

Vav1 (VAV1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only