163,445 results

1. phL1A-pcDNA3

   Plasmid

   #12307

   Depositor

   Vance Lemmon

   Article

   Hlavin et al Genomics. 1991 Oct . 11(2):416-23.

   Insert

   L1CAM (L1CAM Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX4T-E2-25K

   Plasmid

   #63572

   Purpose

   Expression of human Ubiquitin conjugating enzyme E2-25K

   Depositor

   Titia Sixma

   Article

   Pichler et al Nat Struct Mol Biol. 2005 Mar;12(3):264-

   Insert

   UBE2-25K (UBA7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Isoform 1 (200 resiudes)

   Promoter

   tac

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

3. FLAG-NLRC5

   Plasmid

   #37521

   Depositor

   Thomas Kufer

   Article

   Neerincx et al J Biol Chem. 2010 Aug 20;285(34):26223-3

   Insert

   NLRC5 (NLRC5 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tet-pLKO-puro

   Plasmid

   #21915

   Purpose

   3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.

   Depositor

   Dmitri Wiederschain

   Article

   Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMD2.G-K47Q/R354Q

   Plasmid

   #207323

   Purpose

   VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   VSV-G (VSIVgp4 Vesicular Stomatitis Virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47Q/R354Q

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF6-Lck-TurboID

   Plasmid

   #159433

   Purpose

   expresses Lck-TurboID in mammalian cells

   Depositor

   Arthur Salomon

   Article

   Chua et al J Proteome Res. 2020 Nov 13. doi: 10.102

   Inserts

   Lck (LCK Human)
   TurboID (BirA mutant)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   EF-1a

   Available since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScarlet-N1

   Plasmid

   #128060

   Purpose

   Mammalian expression vector for C terminal mScarlet-I fusions

   Depositor

   Oskar Laur

   Type

   Empty backbone

   Use

   Tags

   mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-mStayGold-EFS-Puro

   Plasmid

   #227256

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRham-CALM1

   Plasmid

   #182500

   Purpose

   Bacterial expression of the Homo sapiens calmodulin, CALM1

   Depositor

   Dan Mulvihill

   Article

   Baker et al FEBS Lett. 2022 Jan 31. doi: 10.1002/187

   Insert

   CALM1 (CALM1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rhamnose

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-Hb9-GFP

    Plasmid

    #37080

    Purpose

    3rd generation lentiviral transfer plasmid for Hb9 GFP

    Depositor

    Fred Gage

    Article

    Marchetto et al Cell Stem Cell. 2008 Dec 4;3(6):649-57.

    Insert

    Hb9 GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Hb9

    Available since

    Oct. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. TfR-sfGFP-myc tag-SpyCatcher003

    Plasmid

    #133451

    Purpose

    Expresses SpyCatcher003 for display at the cell surface of mammalian cells, with superfolder GFP for visualization and myc tag for antibody detection

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    TfR-sfGFP-myc tag-SpyCatcher003
    

    Use

    Tags

    GSSGS and myc tag

    Expression

    Mammalian

    Mutation

    Contains C20 and A23 mutations that improve plasm…

    Promoter

    CMV

    Available since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LifeAct-HaloTag

    Plasmid

    #176105

    Purpose

    F-actin marker

    Depositor

    Dorus Gadella

    Insert

    LifeAct
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216246

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgAAVS1.5
    

    Use

    CRISPR; Human safe harbor locus (a avs1) site-spe…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6b:sgRNA#3

    Plasmid

    #64247

    Purpose

    expresses sgRNA under U6b promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCaggs-mGold2s

    Plasmid

    #231763

    Purpose

    Expression of mGold2s in mammalian cells

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGold2s is mGold with V1A;V22I;H77N;Q80R;K101E;D1…

    Promoter

    Actin promoter with CMV enhancer

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag-HA-USP5

    Plasmid

    #22590

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP5 (USP5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6c:sgRNA#4

    Plasmid

    #64248

    Purpose

    expresses sgRNA under U6c promoter

    Depositor

    Wenbiao Chen

    Article

    Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX puro TRIM21-GFP

    Plasmid

    #116941

    Purpose

    Expression of TRIM21-GFP fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    TRIM21 - GFP (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3/Flag-WTAP

    Plasmid

    #53741

    Purpose

    For expression of WTAP (Wilms tumor 1 associated protein) in human cells

    Depositor

    Chuan He

    Article

    Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

    Insert

    Wilms tumor 1 associated protein (WTAP Human)
    

    Use

    Tags

    FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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