We narrowed to 7,518 results for: Lif

1. pGL-p27UTR

   Plasmid

   #20881

   Depositor

   Robert Blelloch

   Article

   Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

   Insert

   p27 3'UTR (Cdkn1b Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

2. sgCtr- LentiCRISPRv2

   Plasmid

   #107402

   Purpose

   Lentiviral expression of Cas9 and a control gRNA

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   control gRNA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pKEK3395

   Plasmid

   #234079

   Purpose

   CCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.

   Depositor

   Karl Klose

   Article

   Armstrong et al Appl Environ Microbiol. 2025 May 12:e000

   Insert

   YukonOFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pJ23100-BsaI

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p212 pCMV-EGFP/RFP

   Plasmid

   #8389

   Purpose

   Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of RFP.

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   floxed EGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHSE901

   Plasmid

   #91708

   Purpose

   CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, HygR

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXSE901B

   Plasmid

   #91713

   Purpose

   CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance

   Depositor

   Qi-Jun Chen

   Article

   Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. WT PBRM1- TRIPZ-neo

   Plasmid

   #107406

   Purpose

   Mammalian expression, inducible PBRM1

   Depositor

   William Kaelin

   Article

   Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

   Insert

   PBRM1 (PBRM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7

   Plasmid

   #121944

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   hU6-Sirius-8XMS2-mU6-Sirius-8XPP7 Cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   no

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoters

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEZ-1

   Plasmid

   #51868

   Purpose

   Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

   Depositor

   Meng-Qiu Dong

   Article

   Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

   Insert

   psdhb-1::mtLS-cpYFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   sdhb-1

   Available since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pX459-sgAAVS1

    Plasmid

    #184403

    Purpose

    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT

    Depositor

    Robert Bradley

    Article

    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:

    Insert

    AAVS1-specific sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCoofy55

    Plasmid

    #122007

    Purpose

    Mammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His10-SumoStar (Life Sensors) and Multi2: dependi…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMA2640

    Plasmid

    #25434

    Purpose

    Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection

    Depositor

    Mikhail Alexeyev

    Article

    Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

    Insert

    reverse tetracycline-regulated transactivator Advanced
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET251-pUC 12xMS2V6 Loxp KANr Loxp

    Plasmid

    #104392

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    12xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SFG.CNb30_opt.IRES.eGFP

    Plasmid

    #22493

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

    Insert

    Codon optimised Calcineurin B mutant 30 (PPP3R1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    L124T; K125-LA-Ins

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBad-mCherry-XL (R125K)

    Plasmid

    #188054

    Purpose

    Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

    Insert

    mCherry-XL (R125K)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    differs from mCherry by R125K, W143S, I161V, Q163…

    Promoter

    araBAD

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

    Plasmid

    #175547

    Purpose

    Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    H2B-HaloTag7 and Tomm20-HaloTag9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAtetO_6xHis-Bst-LF

    Plasmid

    #145799

    Purpose

    Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

    Depositor

    Andrew Ellington

    Article

    Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

    Insert

    Bst-LF
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tet PA

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pPLP4HS-mCf

    Plasmid

    #229988

    Purpose

    LIC vector for improved multigene expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. His6 CP tag and Twin-strep MP tag for AC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Use

    Tags

    His6 and Twin-Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPLP2S-mCf

    Plasmid

    #229986

    Purpose

    LIC vector for improved polycistronic expression of MS2 Phage-Like Particles in bacteria with packaged control sequence. Strep-tag II CP tag for Strep-Tactin AC purification.

    Depositor

    Barry Lutz

    Article

    Kline et al Biotechnol Biotechnol Equip. 2025 Feb;39

    Type

    Empty backbone

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBW103ParsR-Amp30C

    Plasmid

    #78638

    Purpose

    pBW100ParsR carrying 30hrpR-30hrpS-t-hrpL-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    30hrpR-30hrpS-t-hrpL-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SFG.wtCNb_opt.IRES.eGFP

    Plasmid

    #22492

    Depositor

    Martin Pule

    Article

    Brewin et al Blood. 2009 Nov 26;114(23):4792-803. doi

    Insert

    Codon optimised wild type Calcineurin B (PPP3R1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBW101ParsR-gfp

    Plasmid

    #78636

    Purpose

    pSB3K3 carrying J101-rbs32-arsR-t-ParsR-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wang et al Nucleic Acids Res. 2014 Aug;42(14):9484-

    Insert

    J101-rbs32-arsR-t-ParsR-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    see insert

    Available since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pUPD2+p35SoTMV

    Plasmid

    #170876

    Purpose

    Phytobrick - p35SoTMV

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    p35SoTMV
    

    Use

    Tags

    Expression

    Plant

    Mutation

    214C>T and 229C>T to remove BbsI restrictio…

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLX-EF1a_NKX2-1_Short_V5-GFP

    Plasmid

    #221497

    Purpose

    NKX2-1-GFP fusion protein lentiviral overexpression using an EF1a promoter

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5-linker-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPBbsr2-EKARrEV-NLS

    Plasmid

    #173855

    Purpose

    Encoding EKARrEV-NLS.

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    EKARrEV-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCS2-mCherry-Rab11a

    Plasmid

    #184035

    Purpose

    Expresses mCherry Rab11a in mammalian cells and zebrafish embryos

    Depositor

    Heidi Hehnly

    Article

    Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

    Insert

    Rab11a
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE94

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. p210 pCMV-CREM

    Plasmid

    #8395

    Purpose

    Cre with chimeric/β-globin intron within the Cre coding sequence

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    CMV

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLH-sgRNA-Sirius-4X(MS2-PP7)

    Plasmid

    #121941

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-4X(MS2-PP7)
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GFP-Rabenosyn-5

    Plasmid

    #37538

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    Rabenosyn5 (RBSN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3 CDK7 HA (P#633)

    Plasmid

    #14647

    Depositor

    Matija Peterlin

    Article

    Kanazawa et al Oncogene. 2003 Aug 28. 22(36):5707-11.

    Insert

    Cdk7 (CDK7 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge