Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 9,302 results for: BLI;

Clear keywords
Showing: 7451 - 7500 of 9302 results

  2. pDule-Tet3.0

    Plasmid
    #174080
    Purpose
    Tetrazine 3.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. barkeri for expression in E.coli.
    Depositor
    Ryan Mehl
    Article
    Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-
    Insert
    Tet3.0 tRNA synthetase and tRNA
    Expression
    Bacterial
    Mutation
    L270G, Y271L, N311G, C313A
    Available Since
    Jan. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    174080-plasmid-map-sequence-id-351340

    Enlarge

  3. EGFR(L858R,T790M)-NanoLuc Fusion Vector

    Plasmid
    #237199
    Purpose
    Express EGFR(L858R,T790M)-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    EGFR (EGFR Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    L858R/T790M double mutations
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237199-plasmid-map-sequence-id-471864

    Enlarge

  4. 7MEQ

    Plasmid
    #176412
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    TMPRSS2 – transmembrane serine protease 2 (TMPRSS2 Human)
    Use
    Baculovirus expresssion
    Tags
    8 x His Tag and Honeybee melittin signal
    Mutation
    etmprss2_S251D/R252D/Q253D/S254D/R255K
    Promoter
    Polyhedrin
    Available Since
    Oct. 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    176412-plasmid-map-sequence-id-345854

    Enlarge

  5. pFP.R268

    Plasmid
    #138180
    Purpose
    Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(LNR).gp41-8.(SAV)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138180-plasmid-map-sequence-id-273503

    Enlarge

  6. pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)

    Plasmid
    #117134
    Purpose
    All-in-one expression vector for Cas9 and gRNA against CD44
    Depositor
    Kosuke Yusa
    Insert
    gCD44 v2 (Cd44 Synthetic)
    Use
    CRISPR and Lentiviral
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, human EF1a promoter
    Available Since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117134-plasmid-map-sequence-id-226805

    Enlarge

  10. NanoBRET Assay Vector, NSD3-NanoLuc

    Plasmid
    #237008
    Purpose
    Express NSD3-NanoLuc(R) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    NSD3 (NSD3 Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237008-plasmid-map-sequence-id-476796

    Enlarge

  14. AICSDP-60: SOX2-mEGFP

    Plasmid
    #124606
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SOX2
    Depositor
    Allen Institute for Cell Science
    Insert
    SOX2 Homology Arms with linker-mEGFP (SOX2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    May 13, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    124606-plasmid-map-sequence-id-243801

    Enlarge

  15. HTATIP (2OU2)

    Plasmid
    #33338
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    HTATIP (KAT5 Human)
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pET29b Tau Cys

    Plasmid
    #108867
    Purpose
    Expresses human Tau with a mutated cysteine
    Depositor
    Gabriele Kaminski Schierle
    Insert
    Tau (MAPT Human)
    Expression
    Bacterial
    Mutation
    I260C C291A C322A to allow maleimide dye labelling
    Promoter
    T7
    Available Since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    108867-plasmid-map-sequence-id-209739

    Enlarge

  17. pOTTC1740 - pAAV SYN1 DIO HA-hM4D(Gi)

    Plasmid
    #125146
    Purpose
    An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter
    Depositor
    Christopher Richie
    Insert
    HA-tagged hM4D(Gi) (CHRM4 Human)
    Use
    AAV and Cre/Lox
    Tags
    HA
    Expression
    Mammalian
    Promoter
    SYN1
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125146-plasmid-map-sequence-id-244357

    Enlarge

  18. CRAF:CRAF BiBiT Vector

    Plasmid
    #238808
    Purpose
    Express CRAF-LgBiT_CRAF-SmBiT Fusion Proteins in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    CRAF (RAF1 Human)
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238808-plasmid-map-sequence-id-479918

    Enlarge

  20. pCAG-GluA1-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid
    #230058
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA1 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA1 single chain variable fragment (scFv)
    Tags
    6X HIS, HRV 3C PreScission protease, HaloTag, and…
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230058-plasmid-map-sequence-id-460119

    Enlarge

  21. pCAG-GluA2-scFv-Halo-HRV-BiotinAP-HIS

    Plasmid
    #230059
    Purpose
    Expresses a single chain variable fragment (scFv) for anti-GluA2 fused to Halotag with an HRV (PreScission) protease cleavage site, biotin acceptor peptide and 6XHIS purification tag.
    Depositor
    Matthew Kennedy
    Insert
    Anti-GluA2 single chain variable fragment (scFv)
    Tags
    6X HIS, HRV 3C PreScission protease, HaloTag, and…
    Expression
    Mammalian
    Promoter
    Chicken beta actin (CAG)
    Available Since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    230059-plasmid-map-sequence-id-460118

    Enlarge

  23. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198048
    Purpose
    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198048-plasmid-map-sequence-id-389382

    Enlarge

  26. AICSDP-116:PCNA-mEGFP

    Plasmid
    #170818
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA
    Depositor
    Allen Institute for Cell Science
    Insert
    PCNA Homology Arms with mEGFP-linker (PCNA Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    June 11, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170818-plasmid-map-sequence-id-334731

    Enlarge

  27. AICSDP-166:CDH1-mEGFP

    Plasmid
    #193921
    Purpose
    Homology arms and LE-mEGFP sequence for C-terminus tagging of human cadherin 1
    Depositor
    Allen Institute for Cell Science
    Insert
    cadherin 1 Homology Arms with LE-mEGFP (CDH1 Human)
    Use
    AAV and CRISPR; Donor template
    Tags
    linker-mEGFP
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Dec. 22, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193921-plasmid-map-sequence-id-485363

    Enlarge

  28. CMV_Mex3a_mutRING_t2a_mCherry

    Plasmid
    #223245
    Purpose
    Expression of mutant RING Mex3a in mammalian cells with t2a cleaved mCherry
    Depositor
    Stavros Lomvardas
    Insert
    Mex3a (Mex3a Mouse)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    4 point mutations in RING Domain: Cys 484 Ala; Hi…
    Promoter
    CMV
    Available Since
    May 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    223245-plasmid-map-sequence-id-441774

    Enlarge

  30. pTipi2.1_Anti-GCN4 [IPI-C11L34] Mouse/Rabbit HC

    Plasmid
    #221353
    Purpose
    Mammalian expression of Anti-GCN4 [IPI-C11L34] Mouse/Rabbit HC; to be used with Anti-GCN4 [IPI-C11L34] light chain (Plasmid 221354) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-GCN4 [IPI-C11L34] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221353-plasmid-map-sequence-id-436802

    Enlarge

  31. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Expression
    Bacterial and Mammalian
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    113631-plasmid-map-sequence-id-222216

    Enlarge

  32. AICSDP-80:UBTF-HaloTag

    Plasmid
    #133963
    Purpose
    Homology arms and HaloTag-linker sequence for N-terminus tagging of human UBTF
    Depositor
    Allen Institute for Cell Science
    Insert
    UBTF Homology Arms with HaloTag-linker (UBTF Human)
    Use
    CRISPR
    Tags
    HaloTag-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    133963-plasmid-map-sequence-id-264347

    Enlarge

  33. Lenti-DAG1

    Plasmid
    #205149
    Purpose
    Overexpress DAG1
    Depositor
    Monkol Lek
    Insert
    Dystroglycan 1 (DAG1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    Carries common missense variant c.41C>G (p.Ser…
    Promoter
    EF-1a
    Available Since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    205149-plasmid-map-sequence-id-405998

    Enlarge

  34. IGF2BP3-HaloTag-HiBiT Fusion Vector

    Plasmid
    #238631
    Purpose
    Express IGF2BP3-HaloTag-HiBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    IGF2BP3 (IGF2BP3 Human)
    Tags
    HaloTag (R)-HiBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238631-plasmid-map-sequence-id-478492

    Enlarge

  35. pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO

    Plasmid
    #199781
    Purpose
    Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).
    Depositor
    Sascha Martens
    Insert
    NEMO (IKBKG Human)
    Tags
    GST-Thrombin-TEV-EGFP
    Expression
    Bacterial
    Available Since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    199781-plasmid-map-sequence-id-418258

    Enlarge

  36. Membrane EGFP

    Plasmid
    #198056
    Purpose
    Plasma membrane localizing EGFP using Human LCK proto-oncogene sequence
    Depositor
    Amro Hamdoun
    Insert
    LCK membrane EGFP (LCK Synthetic, A. victoria, Human)
    Use
    Sea urchin, mammal, zebrafish
    Tags
    EGFP
    Mutation
    Tandem repeat of N-terminal LCK membrane targetin…
    Promoter
    sp6
    Available Since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    198056-plasmid-map-sequence-id-389155

    Enlarge

  37. AICSDP-079:DCP1A-mEGFP

    Plasmid
    #193922
    Purpose
    Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A
    Depositor
    Allen Institute for Cell Science
    Insert
    DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Dec. 22, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193922-plasmid-map-sequence-id-380539

    Enlarge

  38. pL1P3 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198047
    Purpose
    MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available Since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198047-plasmid-map-sequence-id-389251

    Enlarge

  40. TAF1

    Plasmid
    #39117
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TAF1 (TAF1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  41. AICSDP-57: SMC1A-mEGFP

    Plasmid
    #114406
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A
    Depositor
    Allen Institute for Cell Science
    Insert
    SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114406-plasmid-map-sequence-id-220833

    Enlarge

  42. PiggyBac-TDP-43deltaNLS-V5-APEX

    Plasmid
    #229081
    Purpose
    Doxycycline-inducible expression of NLS-mutated TDP-43 fused to V5-tag and APEX for proximity labeling, containing BFP in the backbone
    Depositor
    Eran Hornstein
    Insert
    TDP-43deltaNLS (TARDBP Human)
    Tags
    APEX, BFP, and V5
    Expression
    Mammalian
    Mutation
    K82A, R83A, K84A, K95A, K97A and R98A for mutatio…
    Promoter
    TRE3G
    Available Since
    July 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    229081-plasmid-map-sequence-id-457517

    Enlarge

  44. ITCH

    Plasmid
    #36197
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ITCH (ITCH Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains HECT domain
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  48. pTipi2.1_Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC

    Plasmid
    #221361
    Purpose
    Mammalian expression of Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC; to be used with Anti-ProC [IPI-HPC-4] light chain (Plasmid 221362) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221361-plasmid-map-sequence-id-436824

    Enlarge

  49. AAV-sCAG-eGFP-ACAP1

    Plasmid
    #203800
    Purpose
    AAV vector plasmid expressing human ACAP1 fused to eGFP under a short variant of the CAG (sCAG) promoter
    Depositor
    Richard Eva, Bart Nieuwenhuis
    Insert
    ACAP1 (ACAP1 Human)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Available Since
    Oct. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    203800-plasmid-map-sequence-id-410892

    Enlarge

  50. pOTTC1761 - pAAV SYN1 DIO HA-hM3D(Gq)

    Plasmid
    #125147
    Purpose
    An adeno-associated viral vector expressing Cre-dependent HA-tagged inhibitory DREADD (hM3D(Gq)) from a synapsin promoter
    Depositor
    Christopher Richie
    Insert
    HA-tagged hM3D(Gq) (CHRM3 Human)
    Use
    AAV and Cre/Lox
    Tags
    HA
    Expression
    Mammalian
    Promoter
    SYN1
    Available Since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    125147-plasmid-map-sequence-id-244359

    Enlarge

Showing: 7451 - 7500 of 9302 results