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FUW-SOK

Plasmid

#20327

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Sox2-F2A-Oct4-T2A-Klf4 (Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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Ai-LV (CS-TRE-mOKS-PRE-Ubc-rtTA-I2G)

Plasmid

#198058

Purpose

Generation of rodent iPS cells from somatic cells

Depositor

Hiromitsu Nakauchi

Article

Yamaguchi et al Methods Mol Biol. 2014;1210:143-50. doi:

Insert

mOct4, mKlf4, mSox2 (Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe_LYSET_isoform1

Plasmid

#246080

Purpose

Stable expression of LYSET isoform 1 (long isoform)

Depositor

Wilhelm Palm

Article

Pechincha et al Science. 2022 Oct 7;378(6615):eabn5637.

Insert

LYSET (LYSET Human)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ClipF-HA-2a-Hu VGAT sesRNA-2a-smV5-2a-tTA2-WPRE

Plasmid

#239031

Purpose

Expression of ClipF, sesRNA in mammalian cells, with smV5 and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for Human VGAT (SLC32A1 Human)

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pBG100-TCPTP

Plasmid

#33365

Depositor

Ambra Pozzi

Article

Borza et al J Biol Chem. 2010 Dec 17;285(51):40114-2

Insert

TCPTP-45 (PTPN2 Human)

Tags

6xHIS - cleavable

Expression

Bacterial

Promoter

T7lac

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pET14b_tev_PSGU

Plasmid

#59738

Purpose

Expresses a.a. 417-887 of human ZO-1

Depositor

Alan Fanning

Article

Nomme et al J Biol Chem. 2011 Dec 16;286(50):43352-6

Insert

zonula occludens-1 (TJP1 Human)

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Promoter

T7

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
Split 'n coexpress CUL3-RBX1

Plasmid

#52293

Purpose

bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1

Depositor

Brenda Schulman

Article

Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1

Inserts

Cul3 N-terminal domain (CUL3 Human)
Cul3 C-terminal domain (CUL3 Human)
RBX1 (Rbx1 Mouse)

Tags

GST, IRES, and TEV

Expression

Bacterial

Mutation

contains aa 1-309, contains aa 310-768, and conta…

Promoter

IRES and tac

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only
Tol2-mpeg1-dendra2

Plasmid

#51462

Purpose

expresses photoconvertible dendra in macrophages

Depositor

Anna Huttenlocher

Article

Harvie et al Infect Immun. 2013 Jan;81(1):110-21. doi

Insert

mpeg-dendra

Expression

Bacterial

Promoter

mpeg1

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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pRL128

Plasmid

#40180

Depositor

Eric Cascales

Article

Gueguen et al Appl Environ Microbiol. 2013 Jan;79(1):3

Inserts

Ptac-lacO
araC-pBAD

Promoter

BAD and tac

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pYS2

Plasmid

#117490

Purpose

Generation of unmarked gene deletions in Mycobacterium smegmatis

Depositor

Eyal Gur

Article

Shenkerman et al Gene. 2014 Jan 1;533(1):374-8. doi: 10.1

Insert

gfp, hyg

Use

Deletion generation

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pBA2SkIKmA2

Plasmid

#39214

Depositor

Anastasios Melis

Article

Lindberg et al Metab Eng. 2010 Jan;12(1):70-9. Epub 200

Insert

Synechocystis kudzu IspS

Use

Synthetic Biology

Promoter

psbA2

Available Since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pLEW100cre-EP1-6G

Plasmid

#33344

Depositor

George Cross

Article

Scahill et al Mol Biochem Parasitol. 2008 Jan;157(1):7

Insert

cre recombinase

Use

Trypanosoma brucei expression vector

Available Since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only

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MDH1_KW

Plasmid

#224145

Purpose

IPTG-inducible expression of C. elegans protein MDH-1 with a chitin tag

Depositor

Katherine Walstrom

Article

Thomas et al Biochim Biophys Acta Proteins Proteom. 2

Insert

MDH-1 (F46E10.10 Nematode)

Tags

Ala-intein-CBD

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCX-TRI-2A

Plasmid

#74674

Purpose

mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)

Depositor

Roberto Giovannoni

Article

Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

Inserts

HO1 (HMOX1 Human)
CD73 (NT5E Human)
CD39 (ENTPD1 Human)

Tags

F2A

Expression

Mammalian

Promoter

pCAGGS

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pAd-PRL.Cre

Plasmid

#192937

Purpose

Adenoviral expression vector encoding sgRNAs targeting Trp53, Rb1 and Rbl2 and Cre

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Inserts

Rb1 (Rb1 Mouse)
Trp53 (Trp53 Mouse)
Rbl2 (Rbl2 Mouse)

Use

Adenoviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

Plasmid

#223959

Purpose

Dual fluorescent reporter for histone H2B and for APC/C activity

Depositor

Hee Won Yang

Article

Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

Insert

H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)

Use

Lentiviral

Tags

iRFP670 and mCerulean

Expression

Mammalian

Promoter

EF-1a

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

Plasmid

#159692

Purpose

Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

Depositor

Dominic Esposito

Article

Procter et al Protein Expr Purif. 2018 Nov;151:99-105.

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L (KRAS Human)

Tags

His6-MBP-tev

Expression

Insect

Mutation

M188L

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-Cas9-2A-BFP

Plasmid

#164662

Purpose

Retroviral introduction of Cas9 into mammalian cell line coupled to expression of blue fluorescent protein

Depositor

Daniel Hodson

Article

Caeser et al Nat Commun. 2019 Oct 4;10(1):4543. doi:

Inserts

Cas9
BFP

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

MSSV_LTR

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On-shRNA-Control

Plasmid

#98398

Purpose

Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

Depositor

Roland Friedel

Article

Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

Insert

non-targeting randomized sequence

Use

Lentiviral

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTol2-HuC(elavl3)-CaMPARI2

Plasmid

#137185

Purpose

pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Insert

HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA

Use

Tol2 plasmid for zebrafish

Tags

FLAG, HA, myc

Promoter

HuC(elavl3)

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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