We narrowed to 30,451 results for: Ide

1. VP16-ER beta (short)

   Plasmid

   #11353

   Depositor

   Donald McDonnell

   Article

   Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

   Insert

   ER beta short (ESR2 Human)
   

   Expression

   Mammalian

   Available Since

   March 10, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet

   Plasmid

   #176535

   Purpose

   Non-protein coding locus components from CRISPR-associated IscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB
   

   Expression

   Bacterial

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP TEM4 R130D

   Plasmid

   #58895

   Purpose

   Expresses GFP Tagged full length TEM4 protein with R130D mutation. Please see notes below for information regarding the L722F mutation in TEM4.

   Depositor

   Channing Der

   Article

   Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

   Insert

   TEM4 (ARHGEF17 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R130D, L722F

   Promoter

   CMV

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. PET-SpCas9-HF1-plus-NLS-6xHis

   Plasmid

   #207381

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   SpCas9-HF1-plus
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, Q695A, Q926A, amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET-B-SpCas9-HF1-NLS-6xHis

   Plasmid

   #207385

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-SpCas9-HF1
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-mqgDUX4mqg*

   Plasmid

   #21177

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

   Insert

   DUX4 (DUX4 Human)
   

   Expression

   Bacterial and Mammalian

   Mutation

   Point mutation at nucleotide 6163 (numbering acco…

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pEGFP TEM4 DEL 125-135

   Plasmid

   #58896

   Purpose

   Expresses GFP tagged full length TEM4 protein with amino acids 125 - 135 deleted

   Depositor

   Channing Der

   Article

   Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

   Insert

   TEM4 (ARHGEF17 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deleted aa's 125-135

   Promoter

   CMV

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

   Plasmid

   #176588

   Purpose

   Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   CRISPR-associate IscB (Delaware Bay) locus
   

   Expression

   Bacterial

   Mutation

   Replaced spacer in CRISPR array with Fn spacer

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. B-HeFSpCas9

   Plasmid

   #126766

   Purpose

   Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   B-HeFSpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…

   Promoter

   CBh

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-FLAG-eSpCas9-plus

    Plasmid

    #126774

    Purpose

    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Rat SERCA2b (pMT2)

    Plasmid

    #75184

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    Gary Shull, Jonathan Lytton

    Article

    Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

    Insert

    ATP2A2
    

    Expression

    Mammalian

    Promoter

    adenovirus major late promoter

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFRT-TODestFLAGHA_RBPMS

    Plasmid

    #59388

    Purpose

    Destination vector with RBPMS for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid

    #101743

    Purpose

    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
    

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4, added 9F in FgßF3

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2-mkgDUX4mqg*

    Plasmid

    #21175

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    Point mutation at nucleotide 6163 (numbering acco…

    Available Since

    Aug. 6, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pCS2-mkgDUX4mal*

    Plasmid

    #21172

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    Point mutation at nucleotide 5114 (numbering acco…

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pLenti-X1-Neo-DDRGK1-K-less-HA

    Plasmid

    #139849

    Purpose

    Lentiviral expression of human DDRGK1-K-less-HA

    Depositor

    Jacob Corn

    Article

    Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

    Insert

    DDRGK1 (DDRGK1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    K116R, K120R, K121R,K124R, K128R, K146R, K176R, K…

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. B-eSpCas9

    Plasmid

    #126761

    Purpose

    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A; K1003A; R1060A; amino acids 1005-1013 repl…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. B-evoSpCas9

    Plasmid

    #126765

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-evoSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than evoSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-evoSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    M495V; Y515N; K526E; R661Q; amino acids 1005-1013…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6.3-KLRC1-V5

    Plasmid

    #124093

    Purpose

    KLRC1-V5 tagged expression vector

    Depositor

    J. Chad Brenner

    Article

    Mann et al Oral Oncol. 2023 Nov;146:106562. doi: 10

    Insert

    KLRC1 (KLRC1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    Oct. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. eft-3p:act-5(ptc):tbb2 3' UTR

    Plasmid

    #202331

    Purpose

    The ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.

    Depositor

    Didier Stainier

    Article

    Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.

    Inserts

    eft-3 promoter
    act-5(ptc)
    tbb-2 3' UTR
    

    Tags

    base 238 C -> A mutation causing a PTC at codo…

    Expression

    Worm

    Promoter

    This is a promoter sequence for C. elegans eft-3,…

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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