We narrowed to 12,555 results for: LIC

1. pCherryGFP-CoV2-Inframe-stop

   Plasmid

   #177620

   Purpose

   Dual fluorescent protein reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. GFP is in-frame with mCherry. Thus GFP translation is prevented by stop codon within FSE (negative control).

   Depositor

   Junjie Guo

   Article

   Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

   Insert

   SARS-CoV-2 FSE
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAG-hPPP3CC-FLAG

   Plasmid

   #179162

   Purpose

   Expression vector of human protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

   Depositor

   Masahito Ikawa

   Article

   Miyata et al Science. 2015 Oct 23;350(6259):442-5. do

   Insert

   protein phosphatase 3, catalytic subunit, gamma isoform (PPP3CC Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 5HT2C-RNA2-Flag

   Plasmid

   #79679

   Purpose

   serotonin receptor 2C isoform 2 with C-terminal Flag tag, non-edited isoform

   Depositor

   Stefan Stamm

   Article

   Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

   Insert

   serotonin receptor 2C, RNA2 (HTR2C Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-hSyn-R-iLACCO1.2

   Plasmid

   #208101

   Purpose

   Biosensor for intracellular L-lactate

   Depositor

   Robert Campbell, Marie-Eve Paquet

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-diLACCO1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Seattle_IPDA_control_1_001

   Plasmid

   #167347

   Purpose

   ddPCR gating control for droplets containing all 5 targets (2 in pol)

   Depositor

   Florian Hladik

   Article

   Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

   Insert

   pol_gag_tat_env
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pEF-Bos-FLAG RIG-I MIII

   Plasmid

   #52877

   Purpose

   Expresses RIG-I with a mutation to motif III in mammalian cells

   Depositor

   Curt Horvath

   Article

   Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

   Insert

   DDX58 (RIGI Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T409A, S411A

   Promoter

   Available since

   May 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-R-diLACCO1

   Plasmid

   #208102

   Purpose

   Control biosensor for intracellular L-lactate

   Depositor

   Robert Campbell, Marie-Eve Paquet

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   eLACCO2.1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 1179_pAAV-U6-BbsI-gRNA-CB-EmGFP

   Plasmid

   #89060

   Purpose

   AAV-gRNA cloning vector with GFP reporter

   Depositor

   William Lagor

   Article

   Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

   Insert

   EmGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin with partial CMV enhancer

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AAV SYN flex PSAM4 GlyR IRES EGFP

   Plasmid

   #119741

   Purpose

   Cre-dependent chemogenetic inhibitor expression

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2019 Mar 14. pii: science.aav52

   Has Service

   AAV5 and AAV9

   Insert

   PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   L131G, Q139L, Y217F

   Promoter

   synapsin

   Available since

   March 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 5HT2C-RNA1-FLAG

    Plasmid

    #79678

    Purpose

    serotonin receptor 2C RNA isoform 1 with C-terminal flag tag

    Depositor

    Stefan Stamm

    Article

    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do

    Insert

    5HT2C-RNA1 (HTR2C Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

11. Seattle_IPDA_control_14_004

    Plasmid

    #167350

    Purpose

    ddPCR gating control for droplets containing the env target

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    env
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Seattle_IPDA_control_32_007

    Plasmid

    #167353

    Purpose

    ddPCR gating control for droplets containing 5'pol or 3'pol targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    pol
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Seattle_IPDA_control_17_005

    Plasmid

    #167351

    Purpose

    ddPCR gating control for droplets containing gat and tat targets

    Depositor

    Florian Hladik

    Article

    Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

    Insert

    gat_tat
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBabe_puro_DEST_Flag_PHC3

    Plasmid

    #45262

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    PHC3 (PHC3 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5-FRT-TO-Nsp1

    Plasmid

    #157679

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp1 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pTag-BFP-C-h-Rab4a-c-Myc

    Plasmid

    #79799

    Purpose

    Express TagBFP labeled human Rab4a (GTPase located on recycling endosomal membranes).

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    TagBFP and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pcDNA3-sACt-mCherry-NLS

    Plasmid

    #138214

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NLS (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-HA-deLACCO1-NGR

    Plasmid

    #208097

    Purpose

    Control biosensor for extracellular L-lactate

    Depositor

    Robert Campbell, Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    deLACCO1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pInducer20 TIFA WT

    Plasmid

    #231589

    Purpose

    TIFA WT gene under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    TIFA (TIFA Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    Promoter

    doxycycline

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NSP6-Flag

    Plasmid

    #210345

    Purpose

    Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP6 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-Syn-ArchT-GFP

    Plasmid

    #63141

    Purpose

    AAV-mediated expression of ArchT-GFP under the Syn promoter. Using hGHpA signal.

    Depositor

    Edward Boyden

    Article

    Han et al Front Syst Neurosci. 2011;5:18. Epub 201

    Insert

    ArchT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFRLuc-CoV2-delStem1

    Plasmid

    #177616

    Purpose

    Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. 3-nucleotide deletion in Stem 1 (negative control).

    Depositor

    Junjie Guo

    Article

    Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

    Insert

    SARS-CoV-2 FSE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    3-nucleotide (UAC) deletion in Stem 1

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pB-TetOn-DEST-hEF1a-mODC-rtTA-IRES-NEO (JDW 1130)

    Plasmid

    #229839

    Purpose

    A CAGGS driven, piggybac compatible, tet-on expression vector containing a luciferase reporter followed by a P2A cleavage peptide and H2A fused mCherry for nuclear labeling.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GFP-Epac1 CAAX

    Plasmid

    #118312

    Purpose

    Expression of human Epac1

    Depositor

    Philip Stork

    Article

    Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

    Insert

    Epac1 CAAX (RAPGEF3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    C-terminal 20aa of KiRas

    Promoter

    CMV

    Available since

    Nov. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pU6-neo-TET

    Plasmid

    #51286

    Purpose

    expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    U6 (RNU6-1 Human)
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    U6 upstream enhancer sequence

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEF-Bos-FLAG MDA5 MI

    Plasmid

    #52875

    Purpose

    Expresses MDA5 with a mutation to motif I in mammalian cells

    Depositor

    Curt Horvath

    Article

    Bamming et al J Biol Chem. 2009 Apr 10;284(15):9700-12

    Insert

    IFIH1 (IFIH1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K335A, E658K

    Promoter

    Available since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBabe EGFR Del1(T790M)

    Plasmid

    #32072

    Depositor

    Matthew Meyerson

    Article

    Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

    Insert

    EGFR (EGFR Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Delete E746-A750, T790M

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MSCV-dGFP-JARID1C

    Plasmid

    #74776

    Purpose

    retroviral expression of JARID1C

    Depositor

    Giovanni Tonon

    Article

    Rondinelli et al Nucleic Acids Res. 2015 Mar 11;43(5):256

    Insert

    JARID1C (KDM5C Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

29. p5E-MCS-c-Fos-b-globin (JDW 1237)

    Plasmid

    #229829

    Purpose

    A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    c-fos minimal promoter and b-globin intron
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV SYN PSAM4 GlyR IRES EGFP

    Plasmid

    #119742

    Purpose

    Chemogenetic inhibitor expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Has Service

    AAV5

    Insert

    PSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F

    Promoter

    synapsin

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRK5-FLAG-FLCN

    Plasmid

    #72290

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

    Insert

    FLCN (FLCN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

32. pRK5-FLAG-FNIP2

    Plasmid

    #72294

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

    Insert

    FNIP2 (FNIP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

33. pAAV-hSyn-R-iLACCO1.1

    Plasmid

    #208100

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell, Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAG27

    Plasmid

    #226742

    Purpose

    Expresses a reporter for a mitochondrial matrix protein

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    COX4 (COX4I1 Human)
    

    Use

    Lentiviral

    Tags

    GFP1-10

    Expression

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMKO.1-shCcnb2

    Plasmid

    #162794

    Purpose

    Ccnb2 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Ccnb2 shRNA (Ccnb2 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. mTFP1-Caveolin-C-10

    Plasmid

    #55468

    Purpose

    Localization: Membrane, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Insert

    NM_001753 (CAV1 Human)
    

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. TDP-43_CTD_del321-343

    Plasmid

    #98676

    Purpose

    Expresses 6xHis-tagged C-terminal domain of TDP-43 with a deletion of AA 321-343

    Depositor

    Nicolas Fawzi

    Article

    Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    del321-343

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-gfaABC1D-R-iLACCO1.1

    Plasmid

    #208103

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell, Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    deLACCO1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FLCN-eGFP

    Plasmid

    #72289

    Purpose

    Expresses human FLCN tagged with eGFP at C terminus

    Depositor

    David Sabatini

    Article

    Tsun et al Mol Cell. 2013 Nov 21;52(4):495-505. doi

    Insert

    FLCN (FLCN Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA5-FRT-TO-Nsp13

    Plasmid

    #157712

    Purpose

    mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp13 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

41. pSLQ7534 pHR (hU6-crSURF1-EFS-PuroR-WPRE)

    Plasmid

    #214878

    Purpose

    Lentiviral vector encoding RfxCas13d targeting SURF1 guide array

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Insert

    hU6-crSURF1-EFS-PuroR-WPRE
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. plv-AcGFP-SOD1 (wt)

    Plasmid

    #27138

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Lentiviral

    Tags

    AcGFP

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAV-gfaABC1D-R-diLACCO1

    Plasmid

    #208105

    Purpose

    Control biosensor for intracellular L-lactate

    Depositor

    Robert Campbell, Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-iLACCO1.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pcDNA5-FRT-TO-FH-Nsp16

    Plasmid

    #157724

    Purpose

    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    FH-Nsp16 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Flag-His8

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

45. pcDNA3-sACt-mCherry-NES

    Plasmid

    #138215

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NES (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pAAV-CaMKIIa-HA-eLACCO2.1-NGR

    Plasmid

    #207989

    Purpose

    Biosensor for extracellular L-lactate

    Depositor

    Robert Campbell, Yuki Kambe

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    eLACCO2.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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47. pME Actin Vhh Halo Tag (JDW 1223)

    Plasmid

    #224498

    Purpose

    Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Actin-Vhh-Halo-Tag
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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48. pED9x (dCas9-KRAB-mCherry)

    Plasmid

    #163956

    Purpose

    Lentiviral expression plasmid of sgRNA with mCherry

    Depositor

    Junwei Shi

    Article

    Giles et al Immunity. 2022 Mar 8;55(3):557-574.e7. d

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCMV HA hRB delta CDK + T373

    Plasmid

    #58912

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T373 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T373 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

50. pcDNA5-FRT-TO-Nsp16-HF

    Plasmid

    #157725

    Purpose

    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Nsp16-HF (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His8-Flag

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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