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We narrowed to 11,689 results for: VARS

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Showing: 6041 - 6080 of 11689 results

  2. CaMK2rep-S603A

    Plasmid
    #239623
    Purpose
    Inactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.
    Depositor
    F Javier Díez Guerra
    Article
    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029
    Insert
    SpotNES-sGFP2-SynP3-3xmyc (Syn1 Rat, Synthetic)
    Tags
    Spot, myc, and sGFP2
    Expression
    Mammalian
    Mutation
    changed Serine 603 to Alanine
    Promoter
    CMV
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239623-plasmid-map-sequence-id-476237

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  3. nCaMK2rep-S603A

    Plasmid
    #239625
    Purpose
    Inactive CaMKII activity reporter. The lentiviral plasmid contains a nanobody sequence (PSD95.FingR), 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.
    Depositor
    F Javier Díez Guerra
    Article
    Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029
    Insert
    PSD95.FingR-NES-SynP3-3xmyc (Syn1 Rat, Synthetic)
    Use
    Cre/Lox and Lentiviral
    Tags
    Flag and myc
    Expression
    Mammalian
    Mutation
    Changed serine 603 to Alanine
    Promoter
    hSyn
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    239625-plasmid-map-sequence-id-476241

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  9. pCMV-T7-xCas9(3.7)-P2A-EGFP (RTW3576)

    Plasmid
    #139993
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized xCas9(3.7)(A262T/R324L/S409I/E480K/E543D/M694I/E1219V) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized xCas9(3.7) with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    xCas9(3.7)=A262T/R324L/S409I/E480K/E543D/M694I/E1…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139993-plasmid-map-sequence-id-275508

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  10. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid
    #139089
    Purpose
    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    xCas9-NG
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139089-plasmid-map-sequence-id-273885

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  20. pCMV-RVR-A3A-Y130F

    Plasmid
    #193658
    Purpose
    Expression of base editor RVR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RVR-dLbCas12a-hA3A-Y130F, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-Y130F
    Promoter
    CMV
    Available Since
    March 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    193658-plasmid-map-sequence-id-426532

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  21. pCMV-RVR-A3A-N57G

    Plasmid
    #193657
    Purpose
    Expression of base editor RVR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RVR-dLbCas12a-hA3A-N57G, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K538V/Y542R, hAPOBEC3A-N57G
    Promoter
    CMV
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193657-plasmid-map-sequence-id-422142

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  26. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid
    #140000
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140000-plasmid-map-sequence-id-275479

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  28. pAAV.CAG.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106201
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    CAG
    Available Since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    106201-plasmid-map-sequence-id-201037

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  30. pAAV.hSynapsin.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106178
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    hSynapsin
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106178-plasmid-map-sequence-id-204208

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  39. pCMV-RVR-ABE8e-V106W

    Plasmid
    #193659
    Purpose
    Expression of base editor RVR-ABE8e-V106W in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RVR-dLbCas12a-TadA*8e-V106W, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K538V/Y542R, TadA*8e-V106W
    Promoter
    CMV
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193659-plasmid-map-sequence-id-422134

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Showing: 6041 - 6080 of 11689 results