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pCDNA5/FRT/TO_CEP41_HaloTag9_T2A_EGFP

Plasmid

#169328

Purpose

Expression of HaloTag9 located at the microtubules of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag9 (CEP41 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-EGxxFP-Cetn1

Plasmid

#50717

Purpose

Cetn1 genomic region was placed in pCAG-EGxxFP. Positive control for DSB mediated EGFP reconstitution.

Depositor

Masahito Ikawa

Article

Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

Insert

Cetn1 (Cetn1 Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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Yes-EGFP

Plasmid

#110497

Purpose

Fluorescent Yes

Depositor

Bernardo Mainou

Insert

Yes (YES1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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BCL2L1_pLX307

Plasmid

#98323

Purpose

Lentiviral expression of BCL2L1

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mSTING

Plasmid

#214156

Purpose

Transient expression of STING

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (Sting1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CalR_HaloTag9_KDEL

Plasmid

#175540

Purpose

Expression of HaloTag9 in the endoplasmic reticulum of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CalR-HaloTag9-KDEL

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO-shBRCA1 #2

Plasmid

#44595

Depositor

Eros Lazzerini Denchi

Article

Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

Insert

BRCA1 (Brca1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO_LAMP1_HaloTag9

Plasmid

#175541

Purpose

Lyzosomal expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-mCherry-NLS

Plasmid

#165442

Purpose

Expresses nuclear mCherry from CMV promoter

Depositor

Esteban Engel

Article

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

mCherry-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-human p53-(1-393)

Plasmid

#24860

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-EGFP-NLS

Plasmid

#165441

Purpose

Expresses nuclear EGFP from CMV promoter

Depositor

Esteban Engel

Article

Maturana et al Gene Ther. 2021 Jun 28. pii: 10.1038/s41

Insert

EGFP-NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGEM-HaloTag-UBF

Plasmid

#247351

Purpose

CRISPR/Cas9 donor plasmid to tag human UBF with Halo tag

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

UBF (UBTF) (UBTF Human)

Use

CRISPR

Tags

HaloTag

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-syn-dLight1.3b

Plasmid

#135762

Purpose

For expression of the genetically encoded dopamine sensor dLight1.3b

Depositor

Lin Tian

Article

Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

Has Service

AAV9

Insert

dLight1.3b

Use

AAV

Tags

Flag tag

Expression

Mammalian

Promoter

Synapsin

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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FDP-P10B2-A75-RZ-URA3

Plasmid

#130874

Purpose

Integration cassette for OrthoRep containing mKate driven by promoter 10B2 with a hardcoded polyA tail

Depositor

Chang Liu

Article

Zhong et al ACS Synth Biol. 2018 Dec 21;7(12):2930-2

Insert

mKate

Use

Expression of mkate, leu2(q180*), and ura3 on ort…

Available Since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_HaloTag9_T2A_EGFP

Plasmid

#169326

Purpose

Expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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GST-beta-arrestin1

Plasmid

#36918

Depositor

Robert Lefkowitz

Article

Nobles et al J Biol Chem. 2007 Jul 20;282(29):21370-8

Insert

beta-arrestin1 (Arrb1 Rat)

Tags

GST

Expression

Bacterial

Available Since

June 27, 2012

Availability

Academic Institutions and Nonprofits only
pBR.DT-A

Plasmid

#35955

Depositor

Jacqueline Lees

Article

Danielian et al Oncogene. 2008 Nov 20;27(51):6561-70. Ep

Insert

diphtheria toxin A

Use

Bac recombineering

Promoter

PGK

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xHA-MKK6-EE neo

Plasmid

#47546

Purpose

Lentiviral expression vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

Depositor

Kevin Janes

Article

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MKK6-EE (MAP2K6 Human)

Use

Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

S207E, T211E

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pTREtight2

Plasmid

#19407

Depositor

Markus Ralser

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pMXs-Fos

Plasmid

#131595

Purpose

expression of mouse Fos in mammalian cells

Depositor

Kateri Moore, Filipe Pereira

Article

Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

Insert

Fos (Fos Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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