We narrowed to 22,314 results for: CRISPR

1. pKRG3-mU6-PUb-hSpCas9-pAc

   Plasmid

   #162163

   Purpose

   Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   T2A-pAc

   Expression

   Insect

   Mutation

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP gRNA (BRDN0000561167)

   Plasmid

   #80034

   Purpose

   3rd generation lentiviral gRNA plasmid targeting EGFP

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9::BFP-humanized

   Plasmid

   #44247

   Purpose

   Expression of a catalytically inactive, human codon-optimized Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

   Depositor

   Stanley Qi

   Article

   Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

   Insert

   dCas9 fused to BFP
   

   Use

   CRISPR and Retroviral

   Tags

   2xNLS & tagBFP

   Expression

   Mammalian

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   MSCV LTR promoter

   Available since

   April 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS476-pHAGE-TO-Nme dCas9 3XGFP-SgRNA/Telomere-All-in-one

   Plasmid

   #85716

   Purpose

   Expresses dNmeCas9-3XsfGFP and telomere-targeting Nme sgRNA

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Inserts

   Nme dCas9
   telomere sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   3XsfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO and U6

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

   Plasmid

   #168107

   Purpose

   PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Insert

   Cas9-P2A-mCherry
   

   Use

   CRISPR and Cre/Lox

   Tags

   HA

   Expression

   Mutation

   Promoter

   CAG

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDA_831

   Plasmid

   #216100

   Purpose

   CRISPRa, EF1a-driven dCas9-VP64 (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1255_pAAV-U6-SA-BbsI-MluI-gRNA-CB-SACas9-HA-OLLAS-spA

   Plasmid

   #109320

   Purpose

   Plasmid for AAV SaCas9 Mammalian Expression with a BbsI cloning site for a gRNA

   Depositor

   William Lagor

   Article

   Jarrett et al Arterioscler Thromb Vasc Biol. 2018 Jul

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(Stk11)-PGKpuroBFP-W

   Plasmid

   #105043

   Purpose

   Lentiviral gRNA plasmid targeting mouse Stk11 , co-expression of TagBFP

   Depositor

   Kosuke Yusa

   Article

   Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

   Insert

   Stk11 (Stk11 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB12A

   Plasmid

   #210035

   Purpose

   Contains cloning site for Cas12a gRNA cassette, inducible Cas12a, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas12a
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP gRNA (BRDN0000562805)

    Plasmid

    #80035

    Purpose

    3rd generation lentiviral gRNA plasmid targeting EGFP

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pY001 (pFnCpf1_full)

    Plasmid

    #69973

    Purpose

    Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42337

    Purpose

    Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDonor-tBFP-NLS-Neo (Universal)

    Plasmid

    #80767

    Purpose

    Universal donor vector for CRISPR/Cas9-mediated homology-independent knock-in system.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

    Insert

    PITCh-gRNA#3 targeting sequence (GCATCGTACGCGTACGTGTT)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEL032

    Plasmid

    #137901

    Purpose

    STU2.0 LbCas12a for plant genome editing

    Depositor

    Yong Zhang

    Article

    Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. dpYPQ230-D156R-ABE8

    Plasmid

    #195569

    Purpose

    dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    ecTadA8e-(GSAGSAAGSGEF)x3-dLbCas12a-D156R
    

    Use

    CRISPR; Gateway compatible ectada8e-(gsagsaagsgef…

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    ZmUBI1

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSP469

    Plasmid

    #65771

    Purpose

    Human expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135V, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSP680

    Plasmid

    #65772

    Purpose

    Human expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    D1135E, R1335Q, and T1337R mutations in Cas9

    Promoter

    CMV & T7

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-SpCas9-2A-GFP-noITR

    Plasmid

    #118415

    Purpose

    Cas9 GFP plasmid constitutively expressed from CAG promoter. AAV repeat region removed to increase expression

    Depositor

    Mark Denham

    Article

    Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXPR_123

    Plasmid

    #96919

    Purpose

    for CRISPRi, lentiviral expression of dCas9-p300 2A BlastR.

    Depositor

    David Root, John Doench

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    p300

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSP2133

    Plasmid

    #72249

    Purpose

    Human expression plasmid for SpCas9-HF4 variant: CMV-T7-humanSpCas9-HF4(Y450A, N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF4(Y450A/N497A/R661A/Q695A/Q926A)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    Y450A, N497A, R661A, Q695A, and Q926A

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pRC10-EFS-huLbCpf1-2A-Blast-WPRE

    Plasmid

    #123359

    Purpose

    Lentiviral vector to constitutively express humanized LbCpf1/Cas12a, with blasticidin resistance for selection.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    human codon-optimized Cpf1/Cas12a from Lachnospiraceae bacterium,
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. dpYPQ230-D156R-ABE4

    Plasmid

    #195370

    Purpose

    dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    ecTadA8e-(GGGGS)x6-dLbCas12a-D156R
    

    Use

    CRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…

    Tags

    5' and 3' NLS

    Expression

    Plant

    Mutation

    Promoter

    ZmUBI1

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. dpYPQ230-D156R-CBE1

    Plasmid

    #195367

    Purpose

    dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

    Insert

    hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
    

    Use

    CRISPR; Gateway compatible ha3a/y130f-xten plus 3…

    Tags

    3xflag

    Expression

    Plant

    Mutation

    Promoter

    ZmUBI1

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pYPQ233 (dLbCpf1-SRDX)

    Plasmid

    #86211

    Purpose

    Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor dCpf1 Gateway entry plasmid

    Depositor

    Yiping Qi

    Article

    Tang et al Nat Plants. 2017 Feb 17;3:17018. doi: 10

    Insert

    LbCpf1
    

    Use

    CRISPR

    Tags

    5' and 3' NLS and SRDX repressor

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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25. MSP2135

    Plasmid

    #72248

    Purpose

    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

    Insert

    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A, R661A, Q695A, Q926A, and D1135E

    Promoter

    CMV

    Available since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pYPQ-dpcoCas9-Act3.0

    Plasmid

    #158408

    Purpose

    CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pJZC43

    Plasmid

    #66565

    Purpose

    sgRNA + 2XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 2XPP7
    PCP-VP64 IRES mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKRG3-mU6-PUb-3xFLAG-hSpCas9

    Plasmid

    #162161

    Purpose

    Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. B52 + SMARCAL1 sgSTOP

    Plasmid

    #100715

    Purpose

    B52 plasmid expressing SMARCAL1 sgSTOP (cloned in BbsI site) and containing an empty sgRNA-expression cassette (use BsmBI for cloning)

    Depositor

    Alberto Ciccia

    Article

    Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

    Insert

    sgSTOP targeting SMARCAL1 (cloned using BbsI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoters

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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30. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid

    #140000

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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