We narrowed to 12,322 results for: BASE

1. sgRNA3_NANOG

   Plasmid

   #64155

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FB012

   Plasmid

   #119709

   Purpose

   Complete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMT

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   PtrpC:hph:Ttub
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. JD404: pMVP (L3-L2) BioID2 + WPRE

   Plasmid

   #121797

   Purpose

   pMVP L3-L2 entry plasmid, contains BioID2 + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term BioID2 to gene of interest in lentivirus vectors.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   BioID2 + WPRE
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. LA802: pMVP (L3-L2) AID + WPRE

   Plasmid

   #121794

   Purpose

   pMVP L3-L2 entry plasmid, contains AID + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term AID domain (activated by auxin) to gene in lentivirus vectors

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   AID + WPRE
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. KJ201: pMVP (R4-R3) osTir1 open

   Plasmid

   #121743

   Purpose

   pMVP R4-R3 entry plasmid, contains osTir1 (no stop codon) for 3- or 4-component MultiSite Gateway Pro assembly

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   osTir1 (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   9x myc epitope tag

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. KO801: pMVP (L5-L4) Hygro-P2A

   Plasmid

   #121718

   Purpose

   pMVP L5-L4 entry plasmid, contains Hygro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Hygro selection marker linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Hygro-P2A (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. sgRNA2_NANOG

   Plasmid

   #64154

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTW322

   Plasmid

   #115953

   Purpose

   Destination Vector

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl1 P1 With 3'UTR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTW323

   Plasmid

   #115954

   Purpose

   Destination Vector

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl1 P1 No 3'UTR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTW325

    Plasmid

    #115956

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl1 P2 With 3'UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTW326

    Plasmid

    #115957

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl1 P2 No 3'UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTW327

    Plasmid

    #115958

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl1 P2 End
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTW328

    Plasmid

    #115959

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl1 P3 With E1-3'UTR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-kanMX6-P3nmt1-GST

    Plasmid

    #39286

    Depositor

    Jurg Bahler, John Pringle

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus

    Promoter

    Available since

    Sept. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenCMV-PMMars1CyAVPR2

    Plasmid

    #75226

    Purpose

    Lentiviral transfer vector encoding Mars1Cy-tagged AVPR2

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    Mars1Cy-AVPR2 (AVPR2 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA epitope

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLN553 (FuGW-S(MAFK)p-Sponge7)

    Plasmid

    #105203

    Purpose

    Module 2 - synthetic promoter MAFK drives sponge 7 ,"optimized sponge" expression. (see PMID: 29056342 for detailed information)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    S(MAFK)p-Sponge7
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGS1212

    Plasmid

    #63785

    Purpose

    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Strep-tag II followed by URA3 selection marker

    Depositor

    Monika Golas, Bjoern Sander

    Article

    Rai et al Mol Biotechnol. 2014 Jun 27.

    Insert

    eGFP-Strep-tag II followed by URA3 selection marker
    

    Use

    E. coli cloning vector

    Tags

    eGFP-Strep-tag II

    Expression

    Mutation

    Promoter

    none

    Available since

    April 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pVRa20_2913

    Plasmid

    #49674

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF20_2913
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVRa30_83

    Plasmid

    #49686

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF30_83
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pVRa11_3726

    Plasmid

    #49662

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF11_3726
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pVS2e

    Plasmid

    #234905

    Purpose

    All-in-one plant virus expression vector for virus-induced gene silencing (VIGS).

    Depositor

    Guoli Song

    Article

    Hao et al Plant Commun. 2025 May 19:101379. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVTCL2

    Plasmid

    #234920

    Purpose

    All-in-one subcellar localization vector. Construct containing EGFP tag and plasma membrane-targeted mCherry for co-localization study in plants.

    Depositor

    Guoli Song

    Article

    Hao et al Plant Commun. 2025 May 19:101379. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVTCL1-AtbZIP63

    Plasmid

    #234923

    Purpose

    This all-in-one subcellar localization vector is used to study the nuclear localization of AtbZIP63

    Depositor

    Guoli Song

    Article

    Hao et al Plant Commun. 2025 May 19:101379. doi: 1

    Insert

    AtbZIP63
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pVSe

    Plasmid

    #234896

    Purpose

    All-in-one plant virus expression vector for virus-induced gene silencing (VIGS) and virus-mediated overexpression (VOX)

    Depositor

    Guoli Song

    Article

    Hao et al Plant Commun. 2025 May 19:101379. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVTCL4

    Plasmid

    #234922

    Purpose

    All-in-one subcellar localization vector. Construct containing EGFP tag and Golgi-targeted mCherry for co-localization study in plants.

    Depositor

    Guoli Song

    Article

    Hao et al Plant Commun. 2025 May 19:101379. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pVTCL3

    Plasmid

    #234921

    Purpose

    All-in-one subcellar localization vector. Construct containing EGFP tag and cell endoplasmic reticulum-targeted mCherry for co-localization study in plants.

    Depositor

    Guoli Song

    Article

    Hao et al Plant Commun. 2025 May 19:101379. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEL848

    Plasmid

    #214238

    Purpose

    FrCas9 cytosine base editing plasmid V2.1 in rice

    Depositor

    Yong Zhang

    Article

    He et al Plant Biotechnol J. 2024 Sep;22(9):2488-

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MZB127

    Plasmid

    #217836

    Purpose

    Bacterial expression of human Arf1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    Arf1, rtxA (ARF1 Vibrio cholerae, Human)
    

    Use

    Tags

    10x His and CPD

    Expression

    Bacterial

    Mutation

    Q71L

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MZB160

    Plasmid

    #217841

    Purpose

    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (L8K+Q71L) for pulldown assays

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    10x His, HaloTag, and SUMO (SMT3)

    Expression

    Bacterial

    Mutation

    L8K, Q71L

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA

    Plasmid

    #235326

    Purpose

    PiggyBac integration vector for ComMAND open-loop circuit regulating mRuby2

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLentiX1-[TRE3G-mRuby2-miRE.FF4-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235318

    Purpose

    Lentiviral expression of mRuby2 with 3' microRNA only

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G (3rd-generation Tet system)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pPB-[EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH]-UbC-PuroR-T2A-mGL-SV40pA

    Plasmid

    #235327

    Purpose

    PiggyBac integration vector for ComMAND closed-loop circuit regulating mRuby2

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a (human EF1a)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLentiX1-[TRE3G-mRuby2(miRE.FF4)-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235317

    Purpose

    Lentiviral expression of mRuby2 with intronic microRNA only

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G (3rd-generation Tet system)

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHAGE Flag HA ITPA

    Plasmid

    #237998

    Purpose

    Inducible lentiviral expression of ITPA

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    ITPA (ITPA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHAGE Flag HA MPG

    Plasmid

    #237804

    Purpose

    Lentiviral vector expressing WT MPG

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    MPG (MPG Human)
    

    Use

    Tags

    Flag 3HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pHAGE Flag HA MPG Catalytic Inactive

    Plasmid

    #237807

    Purpose

    Lentiviral vector expressing Catalytic Inactive MPG

    Depositor

    David Pellman

    Article

    Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

    Insert

    MPG (MPG Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMDC32B-B/c

    Plasmid

    #227963

    Purpose

    Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

    Insert

    B/c
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCas3-APOBEC1

    Plasmid

    #229536

    Purpose

    pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    rAPOBEC1
    Cas3
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCas3-CDA1

    Plasmid

    #229534

    Purpose

    pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Inserts

    Cas3
    Cytidine deaminase
    Uracil glycosylase inhibitor
    

    Use

    Tags

    XTEN

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae and codon optimi…

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. MZB224

    Plasmid

    #226998

    Purpose

    Bacterial expression of 10xHis-SUMO (Smt3) fusion to HRV-3C cleavable Halotagged Arf (T31N) for pulldown assays

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    Arf1 (ARF1 Human)
    

    Use

    Tags

    10x His, HaloTag, and SUMO (SMT3)

    Expression

    Bacterial

    Mutation

    T31N

    Promoter

    T7

    Available since

    Jan. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge