We narrowed to 5,876 results for: nls

1. pX330-Flag-HFSpCas9-H840A

   Plasmid

   #80455

   Purpose

   high fidelity SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   HFSpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB)

   Plasmid

   #79785

   Purpose

   genomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a barcode landing pad

   Depositor

   Anthony Zador

   Insert

   MAPP-nL
   

   Use

   Synthetic Biology; Sindbis virus genomic plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-ABE8e-NRCH

   Plasmid

   #165416

   Purpose

   Expresses the base editor ABE8e-NRCH in mammalian cells

   Depositor

   David Liu

   Insert

   ABE8e-NRCH
   

   Use

   Tags

   BP-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-RfxCas13d-Blast-BFP

   Plasmid

   #196726

   Purpose

   Sortable and selectable RfxCas13d.

   Depositor

   Bernhard Schmierer

   Insert

   RfxCas13d-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRha-ABE8e-NRCH

   Plasmid

   #165417

   Purpose

   For bacterial purification of ABE8e-NRCH protein

   Depositor

   David Liu

   Insert

   ABE8e-NRCH
   

   Use

   Tags

   8xHIS and BP-NLS

   Expression

   Bacterial

   Mutation

   Promoter

   pRhaBAD

   Available since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. XR1_pHCLYBL-CAG-LSL-GFP-P2A-RFP

   Plasmid

   #129111

   Purpose

   Homologous recombination vector for excision reporter 1 in the CLYBL locus.

   Depositor

   Bruce Conklin

   Insert

   LSL, eGFP, NLS-RFP
   

   Use

   Cre/Lox and TALEN

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-Flag-HeFSpCas9-H840A

   Plasmid

   #80456

   Purpose

   high fidelity enhanced SpCas9 mammalian expression vector

   Depositor

   Ervin Welker

   Insert

   HeFSpCas9_H840A
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Aug. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-UCOE-EF1a-Zim3-dCas9-P2A-BFP

   Plasmid

   #188777

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR-UCOE-SFFV-Zim3-dCas9-P2A-Hygro

   Plasmid

   #188768

   Purpose

   dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Zim3-dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and Zim3 KRAB-NLS fusion

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. MBP-FUS_FL_12E

    Plasmid

    #98652

    Purpose

    Expresses MBP-tagged full length phosphomimetic FUS

    Depositor

    Nicolas Fawzi

    Article

    Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    12 S/T to E

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEvol-pAzFRS.2.t1

    Plasmid

    #73546

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.2.t1
    pAzFRS.2.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-AR

    Plasmid

    #28235

    Purpose

    Mammalian expression of Androgen receptor fused to EGFP

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry

    Plasmid

    #188766

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-UCOE-EF1a-Zim3-dCas9-BFP

    Plasmid

    #188775

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9-mTagBFP2
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJ4M/TDP-43

    Plasmid

    #104480

    Purpose

    bacterial expression of TDP-43

    Depositor

    Nicolas Fawzi

    Article

    Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-C1-AR Q48

    Plasmid

    #86428

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Poly-glutamine expansion, G130D

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-C1-AR Q0

    Plasmid

    #86427

    Purpose

    Fluorescent human androgen receptor (fused to EGFP) with deleted polyglutamine region

    Depositor

    Michael Mancini

    Article

    Stenoien et al Hum Mol Genet. 1999 May . 8(5):731-41.

    Insert

    androgen receptor (AR) (AR Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of the poly-glutamine expansion

    Promoter

    CMV

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-GFP/Cre

    Plasmid

    #49056

    Purpose

    Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

    Depositor

    Fred Gage

    Article

    Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

    Insert

    Cre
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    EGFP, Myc, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ezh2[FL]-dCas9

    Plasmid

    #100086

    Purpose

    Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

    Depositor

    David Segal

    Article

    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

    Insert

    14056 (Ezh2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLag-NLS-Ezh2[FL]-dCas9-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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