-
Plasmid#201196PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-DIIG
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-SWG
Plasmid#201194PurposeExpresses a peptide from the active site of furin genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-SWG
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher-PEED
Plasmid#201195PurposeExpresses a peptide from the LDL receptor binding site of PCSK9 genetically fused to SpyCatcher001 in bacteriaDepositorInsertSpyCatcher-PEED
UseTagsHisTagExpressionBacterialMutationPromoterT7Available sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
1BR9-BFPSTOP
Plasmid#202055PurposeE. coli expression vector for N-ter GFP11 R9 tagged BFPDepositorInsertBFP
UseTags6xHis and GFP11ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-HO1(51-231)
Plasmid#203486PurposeFor recombinant protein production in Escherichia coli. His-tagged Arabidopsis thaliana HEME OXYGENASE 1 (HO1) without the plastid transit peptide under the control of the T7 promoter.DepositorInsertArabidopsis thaliana HEME OXYGENASE1 (51 aa – 231 aa)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinI997A_TurboID_Venus
Plasmid#200273PurposeLentivirus vector expressing actin-binding-deficient vinculin mutant with co-localization labeler TurboID and fluorescence reporter VenusDepositorInsertVinculinI997A_TurboID_Venus
UseLentiviralTagsExpressionMutationPromoterT7Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b_TypeIC_acrIF2_invivo
Plasmid#196402Purposeexpresses acrIF2 protein targeting CRISPR Cascade Type IC for in vivo assayDepositorInsertanti-CRISPR IF2 protein (orf30 P. aeruginosa)
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28b_TypeIC_acrIC4_invivo
Plasmid#196403Purposeexpresses acrIC4 protein targeting CRISPR Cascade Type IC for in vivo assayDepositorInsertanti-CRISPR IC4 protein
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSrtA-MT4
Plasmid#200306PurposeBacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT4. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtADepositorInserteSrtA-LPETG-MT4 (MT4 Human)
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
eSrtA-MT3
Plasmid#200305PurposeBacterial expression plasmids for self cleavable sortase mediated purification of recombinant MT3. Contains His-tag, SUMO-tag, LPETG sortase recognition motif and eSrtADepositorInserteSrtA-LPETG-MT3 (MT3 Human)
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterT7Available sinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCA528 AMSHLP 16-218
Plasmid#180609PurposeBacterial expression for AMSHLP MIT domain residues 16-218. Internal ID: WISP20-83DepositorInsertAMSHLP residues 16-218
UseTagsHIS-SUMOExpressionBacterialMutationResidues 16-218PromoterT7Available sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
BPK2014-Cas12i-Max
Plasmid#188276Purposeprokaryotic expressionDepositorInsertCas12i-Max
UseCRISPRTagsExpressionBacterialMutationnonePromoterT7Available sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJL1_SUL-I
Plasmid#200172PurposeAmino acids 25-308 of SUL-I with an N-terminal 6xHis-tag followed by a TEV site in the pJL1 vectorDepositorInsertSUL-I
UseTags6x His tag and TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
NeuA.pJL1
Plasmid#199115PurposeIn vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)DepositorInsertN-acylneuraminate cytodiylyltransfease (NeuA)
UseTagsStrep-tagExpressionBacterialMutationPromoterT7Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Derp2.pJL1
Plasmid#199116PurposeIn vitro expression of Der p 2, a major common allergen from Dermatophagoides pteronyssinus (dust mite), with synthetic site for glycosylation encoded (GGNWTT)DepositorInsertDer p 2 dust mite allergen
UseTagsGlyctag, His-tagExpressionBacterialMutationPromoterT7Available sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE80l BCB
Plasmid#196472PurposeProtein expression of SpyCatcher-ELP-CarHC-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcherDepositorInsertBCB
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
p32m3C Trx-CN
Plasmid#196474PurposeProtein expression of Thiolredoxin-3C cleavage site-CarHCNDepositorInsertTrx-CN
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE80l AEC
Plasmid#196473PurposeProtein expression of SpyTag-ELP-CarHCDepositorInsertAEC
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_Uricase
Plasmid#182400PurposeBacterial expression of uricaseDepositorInserturicase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLS.539
Plasmid#184958PurposeTest effect of a1/a2 length on editing fabH using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 12
UseTagsExpressionBacterialMutationfabH donor G954APromoterT7/lacAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRD411
Plasmid#178196PurposesRok overexpressionDepositorInsertsrok
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD231
Plasmid#178195PurposeRok overexpressionDepositorInsertrok
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-eBra-L4
Plasmid#186368PurposeEntry clone with ORF encoding Brachury cis-regulatory region flanked by Gateway recombination sequencesDepositorInsertBrachury notochord enhancer
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-Titf-L4
Plasmid#186367PurposeEntry clone with ORF encoding Titf1 cis-regulatory region flanked by Gateway recombination sequencesDepositorInsertTitf endoderm enhancer
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPgiCas13b
Plasmid#184833PurposeInducible expression of PgiCas13b nuclease in bacteria.DepositorInsertCas13b nuclease from Porphyromonas gingivalis
UseCRISPRTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.543
Plasmid#184960PurposeTest effect of a1/a2 length on editing murF using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, murF G1359A, a1/a2 length: 12
UseTagsExpressionBacterialMutationmurF donor G1359APromoterT7/lacAvailable sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLS.545
Plasmid#184962PurposeTest effect of a1/a2 length on editing priB using retron recombineeringDepositorInsertEco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 12
UseTagsExpressionBacterialMutationpriB donor G1069APromoterT7/lacAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-GFP-9S [rpoA]
Plasmid#176221PurposeExpression of GFP-9S [rpoA]DepositorInsertGFP-9S [rpoA]
UseSynthetic BiologyTagsHis-GFPExpressionMutationPromoterT7Available sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-pchD WT
Plasmid#187973PurposeExpresses salicylate adenylation enzyme PchD from Pseudomonas aeruginosaDepositorInsertpchD WT
UseTagsHexahistagExpressionBacterialMutationPromoterT7Available sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-eGFP-Pep-H
Plasmid#185788PurposeEncodes enhanced green flourescent protein linked to Pep-H self-assembling peptide via a GS-linker sequenceDepositorInserteGFP-Pep4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaA
Plasmid#167810PurposeExpresses His6-tagged BhaA in E. coliDepositorInsertBhaA
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaB1
Plasmid#167811PurposeExpresses His6-tagged BhaB1 in E. coliDepositorInsertBhaB1
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28b-bhaB7
Plasmid#167813PurposeExpresses His6-tagged BhaB7 in E. coliDepositorInsertBhaB7
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15b-bhaC1
Plasmid#167814PurposeExpresses BhaC1 in E. coliDepositorInsertBhaC1
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBlueScript II SK-SNAP donor for Hist1h3g
Plasmid#186932PurposeGenomic targeting of SNAP tag at Hist1h3g C-terminalDepositorInsertSNAP donor for Hist1h3g (H3c8 Mouse)
UseTagsSNAPExpressionMammalianMutationPromoterT7Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET3a-FruK
Plasmid#186256PurposeOverexpression of FruK for purificationDepositorInsert1-phosphofructokinase (fruK E. coli)
UseTagsExpressionBacterialMutationPromoterT7Available sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
SNAP-Kindlin
Plasmid#186136PurposeFor expression of recombinant Snap-labeled kindlin in bacteriaDepositorInsertFERMT2 (FERMT2 Human)
UseTagsHis6-sumoExpressionBacterialMutationPromoterT7Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S7-S83C/Δ157-178
Plasmid#128592PurposeExpresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coliDepositorInsertS7 E.coli ribosomal protein (rpsG E.coli)
UseTagsExpressionBacterialMutationS83C, Δ157-178PromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHBT-Mb(GDx-Clo_L10)
Plasmid#183406PurposeExpresses Monobody GDx-Clo_L10 in E. coliDepositorInsertHis tag-Avi tag-TEV cleavage site-Mb(GDx Clo_L10)
UseTagsHis tag-Avi tag-TEV cleavage siteExpressionBacterialMutationPromoterT7Available sinceMay 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1(K569A,K571A)-StrepII
Plasmid#174426PurposeExpresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationK569A, K571APromoterT7Available sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only