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pILGFP92

Plasmid

#83549

Purpose

Used to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TDH3 promoter-yEGFP

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-SYFP2

Plasmid

#189762

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_dCas9-2xAM

Plasmid

#92220

Purpose

Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

Depositor

Hodaka Fujii

Article

Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

Inserts

Sp-dCas9-2xAM tag
gRNA to be inserted into Bbs I sites

Use

CRISPR and Lentiviral

Tags

2xAM tag

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

CBh and U6

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pUC118-mCtnnb1-FLIP-FlpE-Puro

Plasmid

#84541

Purpose

CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mouse beta-catenin FLIP-FlpE - Puro targeting vector

Use

Cre/Lox and Mouse Targeting

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
pOTTC841 - pAAV minP secNLuc

Plasmid

#82498

Purpose

Longitudinal monitoring of Gaussia and Nano luciferase activities to simultaneously assess ER calcium homeostasis and ER stress in vivo

Depositor

Brandon Harvey

Article

Wires et al PLoS One. 2017 Apr 12;12(4):e0175481. do

Insert

secNLuc

Use

AAV

Expression

Mammalian

Promoter

c-Fos minimal promoter

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-YeNL

Plasmid

#208849

Purpose

Expresses FLAG epitope-tagged YeNL "Yellow" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

YeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-OeNL

Plasmid

#208850

Purpose

Expresses FLAG epitope-tagged OeNL "Orange" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

OeNL Nanolantern

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pENTRY-GFP

Plasmid

#15301

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

hrGFP

Expression

Mammalian

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only
pPRIME-CMV-LNGFR-FF3

Plasmid

#11666

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

LNGFR

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pRRL CAGpNFlag BAF155 ires GFP

Plasmid

#24561

Depositor

Jerry Crabtree

Article

Ho et al Proc Natl Acad Sci U S A. 2009 Mar 31. 1

Insert

BAF155 (Smarcc1 Mouse)

Use

Lentiviral

Tags

Flag

Available Since

July 16, 2010

Availability

Academic Institutions and Nonprofits only
Flag-FOXI1

Plasmid

#153126

Purpose

Expression of Flag tagged human FOXI1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXI1 (FOXI1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pTrcHisB-TRF2

Plasmid

#50488

Purpose

E. Coli N-term HexHis Tagged Protein Expression Vector

Depositor

Jack Griffith

Article

Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

Insert

TRF2 (TERF2 Human)

Tags

6x His, T7 tag, and TEV protease site

Expression

Bacterial

Mutation

Contains amino acids 3-500

Promoter

pTrc

Available Since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Hic1 GFP

Plasmid

#106953

Purpose

Lentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

anti-Hic1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pC-KIT1

Plasmid

#118983

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Expression

Mammalian

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-TagBFP-W

Plasmid

#126687

Purpose

Constitutive, ubiquitous blue fluorescence protein (BFP) expression.

Depositor

Darrell Kotton

Article

Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

Insert

TagBFP

Use

Lentiviral

Promoter

Ef1aL

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-Intb2

Plasmid

#205203

Purpose

This vector encodes of the synCAM (Intb2) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM Intb2 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pEF1alpha-rtTA-Antares

Plasmid

#197587

Purpose

Expresses TET-On reverse transactivator labeled with Antares

Depositor

Mikhail Shapiro

Article

Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

rtTA-P2A-Antares

Tags

Antares

Expression

Mammalian

Promoter

EF-1alpha

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMXS-hs-3xHA-LIN-41

Plasmid

#52717

Purpose

retroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-EGFP-Rheb-IP

Plasmid

#13831

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

Rheb (Rheb Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pSMALB

Plasmid

#161786

Purpose

Gateway destination vector with bidirectional CMV-SFFV promoter for high expression of gene of interest and TagBFP.

Depositor

John Dick, Peter van Galen

Article

van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

Type

Empty backbone

Use

Lentiviral

Tags

TagBFP

Expression

Mammalian

Promoter

Bidirectional minhCMV and SFFV

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pJE53

Plasmid

#194153

Purpose

sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, KmR. Contains mrfp nontargeting control guide sequence.

Depositor

Edward Geisinger

Article

Bai et al J Bacteriol. 2021 May 20;203(12):e005652

Insert

sgRNA with mrfp control guide sequence

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD28 SNIPR

Plasmid

#188358

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD28-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD28-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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gh123

Plasmid

#106790

Purpose

expression of gRNA targeting ST8SIA3

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ST8SIA3 (ST8SIA3 Human)

Expression

Mammalian

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTfR-PATagRFP

Plasmid

#31946

Depositor

Vladislav Verkhusha

Article

Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

Insert

PATagRFP

Tags

TfR

Expression

Mammalian

Mutation

R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

Promoter

CMV

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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FUW-cFos

Plasmid

#193089

Purpose

constitutive expression of mouse cFos in mammalian cells

Depositor

Filipe Pereira

Article

Silverio-Alves et al Nat Commun. 2023 Aug 14;14(1):4645. doi:

Insert

cFos

Use

Lentiviral

Expression

Mammalian

Promoter

Ubc

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV:Flag-GpNLuc

Plasmid

#208840

Purpose

Expresses FLAG epitope-tagged GpNLuc "Green" BRET reporter in mammalian cells.

Depositor

Antonio Amelio

Article

Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

Insert

GpNLuc LumiFluor

Use

Lentiviral

Tags

1xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pTol1:uas-myc-act-map2k2b-2a-tdTomato

Plasmid

#104721

Purpose

UAS-driven expression of activated MEK

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

activated map2k2b (map2k2b Zebrafish)

Use

Zebrafish transposon

Tags

2A-tdTomato and myc tag

Mutation

serines 221 and 225 to aspartic acid

Promoter

10xUAS

Available Since

Jan. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-YR-ICUE-mGold2s

Plasmid

#231789

Purpose

Visualization of cAMP dynamics using YR-ICUE-mGold2s

Depositor

Francois St-Pierre

Article

Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

Insert

YR-ICUE-mGold2s

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pSNRW-Z5-Nluc

Plasmid

#174487

Purpose

Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

Depositor

Richard C. Willson

Article

Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

Insert

Z5-Nluc

Tags

6x Histidine tag (N terminal on insert)

Expression

Bacterial

Promoter

T7lac promoter

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-fPV-GFP

Plasmid

#22914

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

fugu parvalbumin promoter driving GFP

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR- flag-Lag16-DLL1

Plasmid

#205201

Purpose

This vector encodes of the synCAM (DLL-1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM DLL1 and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p15a_Kan_MLS_Rosa26_Short_CRISPRDel

Plasmid

#97008

Purpose

Rosa26 locus targeting vector

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-U6sgp53-U6sgbbSapI-GFAPCre

Plasmid

#100275

Purpose

AAV-CRISPR mediated genome editing in glia / astrocytes

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Type

Empty backbone

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Expression

Mammalian

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB115

Plasmid

#68569

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged Citrine (Citri…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMRS-LacI-dCas9

Plasmid

#220194

Purpose

mini-Tn7 dCas9 expression vector - LacI/pLac promoter

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

LacI/pLac

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR- flag-Lag16-iCAM-1-Tether

Plasmid

#205196

Purpose

This vector encodes of the synCAM Tether (iCAM1) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM iCAM1 Tether and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Vmn2r1-5Hprt-loxP TV

Plasmid

#108079

Purpose

Tergeting vector for Vmn2r1 gene knockout mice (∆C1 strain)

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r1-Hprt (5')-loxP-neo (Vmn2r1 Mouse)

Use

Mouse Targeting

Available Since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDx_mCherry-SYFP2

Plasmid

#189759

Purpose

Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mCherry-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
LPC-flag-REST-FS

Plasmid

#41904

Depositor

Stephen Elledge

Article

Westbrook et al Nature. 2008 Mar 20;452(7185):370-4. doi

Insert

RE1-silencing transcription factor (REST Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

FS mutant

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pN1-CMV-sDarken

Plasmid

#184799

Purpose

Serotonin Sensor sDarken under the control of a CMV Promoter.

Depositor

Olivia Masseck

Article

Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

Insert

sDarken

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 6201 - 6240 of 20363 results

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We narrowed to 20,363 results for: ren