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pCytERM_mScarlet_N1

Plasmid

#85066

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

mScarlet

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pBAD-T-GECO1

Plasmid

#191616

Purpose

Bacterial expression plasmids for Teal Fluorescent Genetically Encoded Calcium Indicator T-GECO1

Depositor

Robert Campbell

Article

Aggarwal et al Neurophotonics. 2024 Apr;11(2):024207. d

Insert

T-GECO1

Tags

His tag

Expression

Bacterial

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERP2-YTHDC2

Plasmid

#222558

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of YTHDC2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

YTHDC2 (YTHDC2 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin A

Plasmid

#8959

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin A (CCNA2 Human)

Expression

Mammalian

Available Since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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Rc/CMV cyclin E

Plasmid

#8963

Depositor

Bob Weinberg

Article

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin E (CCNE1 Human)

Tags

GPI linker

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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HaloTag-LacI-mHP1a

Plasmid

#232637

Purpose

lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with effector protein mouse HP1alpha

Depositor

Karsten Rippe

Insert

HaloTag-LacI-mHP1a (Cbx5 Synthetic, Mouse)

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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2.2

Plasmid

#34885

Depositor

Irvin Chen

Article

Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

Insert

Sindbis virus envelope protein

Use

Lentiviral

Expression

Mammalian

Mutation

SGN UTR in E1 domain

Promoter

CMV

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pQE30-roGFP1-iR

Plasmid

#83314

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iR

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iR betwe…

Promoter

T5

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERP2-DDX4

Plasmid

#222527

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DDX4

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DDX4 (DDX4 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pRc/RSV-m CBP-HA

Plasmid

#16701

Depositor

Richard Goodman

Article

Chrivia et al Nature. 1993 Oct 28. 365(6449):855-9.

Insert

CBP (Crebbp Mouse)

Tags

HA

Expression

Mammalian

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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CMV-tdTomato-P2A-paCaMKII

Plasmid

#165431

Purpose

Expresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

tdTomato-P2A-paCaMKII

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET FlipSuc-90u delta1

Plasmid

#14475

Depositor

Wolf Frommer

Article

Lager et al J Biol Chem. 2006 Oct 13. 281(41):30875-

Insert

FlipThuE W283A

Tags

ECFP and EYFP

Expression

Bacterial

Mutation

N192D, W283A, 13 amino acids deleted from linkers

Promoter

T7

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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N1-G-Ca-FLITS

Plasmid

#191465

Purpose

Genetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime change

Depositor

Dorus Gadella

Article

van der Linden et al eLife 14:RP105086; doi: https://doi.org/

Insert

G-Ca-FLITS

Expression

Mammalian

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TET

Plasmid

#20909

Purpose

piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Gateway Destination Vector

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 2

Plasmid

#128571

Purpose

for cloning identified individual SPECS

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pmScarlet_NES_C1

Plasmid

#85060

Purpose

In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

NES

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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7i sgRNA for 4-μHOM reporter

Plasmid

#113626

Purpose

sgRNA/CAS9 expression plasmid to induce a 3’ double-strand break in the 4-μHOM reporter 8 nts. upstream from the microhomology

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7i sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pGEP163

Plasmid

#137881

Purpose

PiggyBac donor vector for expression of mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

mRFP-T2A-BlasticidinR

Expression

Mammalian

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCytERM_mScarlet-i_N1

Plasmid

#85068

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

mScarlet-i

Expression

Mammalian

Mutation

aa1-29

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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