We narrowed to 144,836 results for: ins

1. pUAS-hActin-luc2

   Plasmid

   #48081

   Purpose

   Luciferase Reporter with Gal4 DNA binding site upstream of hACTB promoter

   Depositor

   Huda Zoghbi

   Article

   Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   removed hsp70 promoter, inserted amplified 615 bp…

   Promoter

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

2. ACS2 Truncated

   Plasmid

   #13748

   Depositor

   John Denu

   Article

   Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

   Insert

   Truncated Acetyl CoA Sythetase 2 (Acss1 Mouse)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids 39–682

   Promoter

   Available since

   Feb. 23, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pFastBac1 PIK3R1-Ni

   Plasmid

   #17744

   Depositor

   Bert Vogelstein

   Article

   Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

   Insert

   PIK3R1-Ni (PIK3R1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Partial cDNA

   Promoter

   Available since

   April 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDM1515

   Plasmid

   #109000

   Purpose

   safe haven knock-in plasmid for the act5 locus, C-terminal GFP tagging

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Type

   Empty backbone

   Use

   Safe haven knock-in plasmid for the act5 locus, c…

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]

   Plasmid

   #71805

   Purpose

   Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

   Depositor

   Bas van Steensel

   Article

   Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

   Insert

   FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. 907H1

   Plasmid

   #160234

   Purpose

   FlightN-CymR-VP16-3X-SV40-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   p-cymene repressor
   Flightin promoter
   

   Use

   Tags

   VP16

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPI458

   Plasmid

   #113212

   Purpose

   safe haven knock-in plasmid for the act5 locus, C-terminal mScarlet tagging

   Depositor

   Rob Kay

   Article

   Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

   Type

   Empty backbone

   Use

   Safe haven knock-in plasmid for the act5 locus, c…

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pT2ADW_2paRAF

   Plasmid

   #129654

   Purpose

   For generating Tg mice expressing the 2P-activatable RAF system with P2A and full-length RAF1

   Depositor

   Michiyuki Matsuda

   Article

   Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

   Insert

   2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSETb-rsGreen1

   Plasmid

   #78180

   Purpose

   Expresses rsGreen1 in E. coli strains.

   Depositor

   Peter Dedecker

   Article

   Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

   Insert

   rsGreen1
   

   Use

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDM1514

    Plasmid

    #108999

    Purpose

    safe haven knock-in plasmid for the act5 locus, N-terminal mCherry tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Safe haven knock-in plasmid for the act5 locus, n…

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQP-2460

    Plasmid

    #138973

    Purpose

    Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Pr-deGFP-MGapt

    Plasmid

    #67734

    Purpose

    OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion

    Depositor

    Richard Murray

    Article

    Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

    Insert

    UTR1-deGFP-MGapt-T500
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    OR2-OR1-Pr

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMOD8A-RGR-r10

    Plasmid

    #74370

    Purpose

    This plasmid constitutively expresses gRNA-r10 from an AHD1 promoter using the RGR design. Integrates in the W303 HIS locus and restores HIS function.

    Depositor

    Eric Klavins

    Article

    Gander et al bioRxiv 041871

    Insert

    RGR-r10
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR221-OSF-TRIM5-21R

    Plasmid

    #79063

    Purpose

    Gateway entry vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5-21R
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    T7

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJK357

    Plasmid

    #71568

    Purpose

    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC)

    Depositor

    T. J. Kappock

    Article

    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

    Insert

    succinyl-CoA:acetate CoA-transferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDM1516

    Plasmid

    #109001

    Purpose

    safe haven knock-in plasmid for the act5 locus, C-terminal mCherry tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Safe haven knock-in plasmid for the act5 locus, c…

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS B2rescue A70D

    Plasmid

    #78658

    Purpose

    Generates B2 inserts suitable for rescue

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS Genet. 2012;8(8):e1002842. doi: 10.

    Insert

    B2rescue cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSEM321 - array co-injection fragment mosTI hygroR

    Plasmid

    #159828

    Purpose

    Single copy or array insertion by MosTI using split hygroR selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    array co-injection fragment mosTI hygroR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSP2266

    Plasmid

    #70705

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KG#59

    Plasmid

    #110929

    Purpose

    Expresses proteins pan-neuronally in C. elegans (entire nervous system)

    Depositor

    Kenneth Miller

    Article

    Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

    Inserts

    rab-3 promoter
    unc-54 3' control region
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRSETC-PITPbeta (splice variant 1)

    Plasmid

    #58270

    Purpose

    bacterial expression of PITP beta (phosphatidylinositol transfer protein beta)

    Depositor

    Shamshad Cockcroft

    Article

    Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

    Insert

    PITP beta (PITPNB Human)
    

    Use

    Tags

    HIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pLKO.shNkx2-1

    Plasmid

    #32400

    Depositor

    Monte Winslow, Tyler Jacks

    Article

    Winslow et al Nature. 2011 May 5. 473(7345):101-4.

    Insert

    shRNA targetting Nkx2-1 (Nkx2-1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

23. 907L

    Plasmid

    #160237

    Purpose

    FlightN-ttgR-VP16-Sv40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    TtgR repressor
    Flightin promoter
    

    Use

    Tags

    VP16

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLIC-lyn11-myc-LOV2GIVe (FA)

    Plasmid

    #170626

    Purpose

    For the mammalian expression of mLOV2GIVe GEF-deficient mutation F1685A

    Depositor

    Mikel Garcia-Marcos

    Article

    Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

    Insert

    Lyn11-myc-LOV2GIVe (FA)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. 2xFRB-myc

    Plasmid

    #20227

    Depositor

    Carolyn Bertozzi

    Article

    Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

    Insert

    2xFRB (MTOR Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    1 FRB copy has a T2098L point mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pAct:dCas9-scFv

    Plasmid

    #78900

    Purpose

    Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    scFV-VP64
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    pActin (Drosophila)

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCK300

    Plasmid

    #87766

    Purpose

    empty control plasmid, three terminators to place inserts between for insulation, ampR, pBR322 origin, lacI

    Depositor

    John Heap

    Article

    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    None

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPI419

    Plasmid

    #113211

    Purpose

    safe haven knock-in plasmid for the act5 locus, N-terminal mScarlet tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Safe haven knock-in plasmid for the act5 locus, n…

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPI460

    Plasmid

    #113204

    Purpose

    Inducible expression vector (dox-on), C-terminal mScarlet tagging

    Depositor

    Rob Kay

    Article

    Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

    Type

    Empty backbone

    Use

    Dictyostelium discoideum inducible expression vec…

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GST-rhodopsin-CTS

    Plasmid

    #86071

    Purpose

    A bacterial expression plasmid encoding rhodopsin ciliary targeting signal with N-terminus GST-tag.

    Depositor

    Lei Lu

    Article

    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

    Insert

    Rhodopsin (RHO Bovine)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    contains the region from 309-348 of Rhodopsin

    Promoter

    tac

    Available since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge