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pFastBac1 PIK3R1

Plasmid

#17743

Depositor

Bert Vogelstein

Article

Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

Insert

PIK3R1 (PIK3R1 Human)

Expression

Insect

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
BPK2301

Plasmid

#65778

Purpose

Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

St1Cas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pSEM321 - array co-injection fragment mosTI hygroR

Plasmid

#159828

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI hygroR

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-rTH-tTA

Plasmid

#133268

Purpose

An AAV genome that expresses tTA from the rat TH promoter

Depositor

Viviana Gradinaru

Article

Robinson et al Elife. 2019 Sep 23;8. pii: 48983. doi: 1

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

rTH

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tiam1-pMag(3x)-iRFP

Plasmid

#67305

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Tiam1-pMag(3x)-iRFP (Tiam1 Synthetic, Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pT2ADWpuro_2paRAF

Plasmid

#129653

Purpose

For generating cell line expressing the 2P-activatable RAF system with P2A and full-length RAF1

Depositor

Michiyuki Matsuda

Article

Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

Insert

2paCRY2-Raf1-2A-CIBN-mScarlet-KRasCAAX

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9

Plasmid

#78903

Purpose

Expresses dCas9 under actin promoter for "Scaffold" CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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MSP1594

Plasmid

#65775

Purpose

Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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raACR-mCerulean3-pAAV2-hSyn

Plasmid

#204966

Purpose

Panneuronal expression of the MsACR1 mutant raACR fused to mCerulean3

Depositor

Peter Hegemann

Article

Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

Insert

raACR

Use

AAV

Tags

mCerulean3

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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PT7-deGFP-MGapt

Plasmid

#67741

Purpose

T7 promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T7term

Use

Synthetic Biology

Expression

Bacterial

Promoter

PT7

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-Raptor K840R

Plasmid

#204418

Purpose

Acetylation mutant for human raptor

Depositor

David Rubinsztein

Article

Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

Insert

Regulatory Associated Protein Of MTOR, Complex 1

Tags

HA and YFP

Expression

Mammalian

Mutation

Lysine 840 to Arginine (K840R)

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-Raptor K1097R

Plasmid

#204417

Purpose

Acetylation mutant for human raptor

Depositor

David Rubinsztein

Article

Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

Insert

Regulatory Associated Protein Of MTOR, Complex 1

Tags

HA and YFP

Expression

Mammalian

Mutation

Lysine 1097 to Arginine (K1097R)

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCOLA banana sensor sfGFP

Plasmid

#107367

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

banana-detecting toehold + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pSEM271 - MCS mosTI NeoR target

Plasmid

#159830

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

MCS mosTI NeoR target

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM254 - sgRNA mosTI neoR

Plasmid

#159831

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI neoR

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM322 - array co-injection fragment mosTI neoR

Plasmid

#159832

Purpose

Single copy or array insertion by MosTI using split NeoR selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI neoR

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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vas-Cas9.874Z

Plasmid

#112687

Purpose

Express hSpCas9 under vasa promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

vasa

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pIE2-DBD

Plasmid

#21602

Depositor

Takahiro Kusakabe

Article

Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

Insert

GAL4 DBD (GAL4 Budding Yeast)

Use

Gateway Cloning

Tags

3xHA and Gateway destination cassette

Expression

Insect

Available Since

March 9, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP_sfCherry2(11)_Keratin

Plasmid

#82604

Purpose

Expresses sfCherry2(11) tagged keratin in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

sfCherry2(11)_Keratin

Expression

Mammalian

Mutation

changed Gly12 to Ala in sfCherry11+

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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CT534

Plasmid

#233682

Purpose

Red light-inducible Vcre-C; Vcre-278C-PhyA

Depositor

Wilson Wong

Article

Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

Insert

Vcre-278C-PhyA

Expression

Mammalian

Mutation

None

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pSN007

Plasmid

#102440

Purpose

For PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)

Depositor

Alejandro Chavez, George Church

Article

Nageshwaran et al J Vis Exp. 2018 Oct 2;(140). doi: 10.379

Insert

7SK paired gRNA insert

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Htb2-GFP

Plasmid

#117669

Purpose

Colorize the nuclei

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

Htb2-GFP

Expression

Yeast

Mutation

Contains the gene encoding the Histone Htb2 from …

Promoter

pGAP

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-His6-TEV-MMS2

Plasmid

#25465

Depositor

Cynthia Wolberger

Article

Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

Insert

MMS2 (MMS2, UBC13 Budding Yeast)

Tags

His6 and MBP

Expression

Bacterial

Available Since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP

Plasmid

#67299

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Tiam1-pMagFast2(3x)-iRFP (Tiam1 Synthetic, Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pC4-RHE-PAS (FRB-PAS)

Plasmid

#80002

Purpose

miRFP PAS domain fused to FRB

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

FRB

Tags

PAS domain of miRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAct:dCas9-VP64

Plasmid

#78901

Purpose

Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

Depositor

Norbert Perrimon

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

dCas9-VP64

Use

CRISPR

Expression

Insect

Promoter

pActin (Drosophila)

Available Since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLinker-AID-Ty-HXGPRT-LoxP

Plasmid

#86667

Purpose

for generation of AID strains using CRISPR tagging technology

Depositor

David Sibley

Article

Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

Insert

Linker-AID-Ty-HGPRT 3'UTR

Use

Expression in toxoplasma gondii

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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HB501: pMVP (L1-L4) RIP

Plasmid

#121688

Purpose

pMVP L1-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Rat Ins1 promoter (RIP; β-cell specific)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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NpT7-5-Erk2

Plasmid

#39230

Depositor

Melanie Cobb

Article

Robbins et al J Biol Chem. 1993 Mar 5;268(7):5097-106.

Insert

Erk2 (Mapk1 Rat)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pDM1209

Plasmid

#108986

Purpose

extrachromosomal expression vector, C-terminal GFP tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum expression vector

Tags

GFP

Available Since

July 19, 2018

Availability

Academic Institutions and Nonprofits only

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