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pCI-neo-HA-hAtg13

Plasmid

#22874

Depositor

Noboru Mizushima

Article

Hosokawa et al Autophagy. 2009 Oct . 5(7):973-9.

Insert

autophagy-related genes 13 (ATG13 Human)

Tags

HA

Expression

Mammalian

Available Since

March 25, 2010

Availability

Academic Institutions and Nonprofits only
pDY1314_RADARS_Luciferase

Plasmid

#193191

Purpose

Express Gaussia Luciferase conditional upon RNA transcript

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

Insert

RADARS Luciferase

Expression

Mammalian

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - AAVS1 sgRNA

Plasmid

#70661

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an AAVS1-targeting sgRNA element from U6 promoter. Lentiviral backbone

Depositor

David Sabatini

Article

Wang et al Science. 2015 Oct 15. pii: aac7041.

Insert

sgRNA against AAVS1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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mPlum-Lifeact-7

Plasmid

#54679

Purpose

Localization: Actin, Excitation: 590, Emission: 649

Depositor

Michael Davidson

Insert

Lifeact-mPlum

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only
GFP-KIF1B beta-FL

Plasmid

#79108

Purpose

Mammalian expression of GFP-KIF1B beta-FL

Depositor

Susanne Schlisio

Article

Chen et al Cancer Discov. 2014 Apr;4(4):434-51. doi

Insert

GFP-KIF1B beta full length (KIF1B Human)

Tags

GFP

Expression

Mammalian

Mutation

exon 25 missing (natural splice varient)

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pMy-MouseCTCFbiotag-T2A-mOrange

Plasmid

#50564

Purpose

Retroviral vector endoding for mOrange and mouse CTCF with a short biotinylation substrate (biotag, Kimetal.,2009) fused to its C-term

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Inserts

Ampicilin
Mouse CTCF Exons

Use

Retroviral

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ353 - MCS(ttTi4391, I)

Plasmid

#34869

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

ttTi4391 targeting

Use

Worm targeting

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-M2-TLR2

Plasmid

#12291

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I and H202Y; signal peptide (aa1-23) replaced …

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
iF59 PB-TO-oNgn2-puro

Plasmid

#204734

Purpose

Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Optimized neurogenin-2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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RCAN1

Plasmid

#65413

Purpose

Full length RCAN1 expression with CMV promoter. Flag tagged.

Depositor

Jeffery Molkentin

Article

Liu et al Nat Cell Biol. 2009 Feb;11(2):154-61. do

Insert

RCAN1 (Rcan1 Mouse)

Tags

Flag tagged

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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pRS406-PHO5-GFP-OSBP PH domain

Plasmid

#58840

Purpose

Expresses GFP-Tagged OSBP PH domain in yeast

Depositor

Tim Levine, Sean Munro

Article

Levine et al Curr Biol. 1998 Jun 18;8(13):729-39.

Insert

Oxysterol-binding Protein PH domain (OSBP Human)

Tags

GFP

Expression

Yeast

Promoter

PHO5

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only
pSicoR human Drosha3

Plasmid

#14768

Depositor

Tyler Jacks

Article

Kumar et al Nat Genet. 2007 May;39(5):673-7. doi: 10

Insert

Drosha shRNA (DROSHA Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PLK4

Plasmid

#20522

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PLK4 (PLK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pGR595

Plasmid

#213785

Purpose

Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

Depositor

Chang Liu

Insert

BadBoy2 TP-DNAP1

Use

Synthetic Biology

Expression

Yeast

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pJG/ Inducible ALPHA MHC

Plasmid

#55792

Purpose

transgene expression driven by inducible ALPHA MHC promoter

Depositor

Jeffrey Robbins

Article

Sanbe et al Circ Res. 2003 Apr 4;92(6):609-16. Epub

Type

Empty backbone

Expression

Mammalian

Promoter

Inducible ALPHA MHC

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only
pLX_HA

Plasmid

#178534

Purpose

Modifies pLX_317 backbone to express c-term HA tag (instead of c-term V5 tag)

Depositor

William Hahn

Article

Pan et al Cell Syst. 2022 Jan 21. pii: S2405-4712(

Type

Empty backbone

Tags

HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCas12f1

Plasmid

#171182

Purpose

Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

Depositor

Kohsuke Honda

Article

Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

Insert

Cas12f1

Use

CRISPR

Expression

Bacterial

Promoter

cas9 promoter from Streptococcus pyogenes

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC6-GLS-3xFLAG

Plasmid

#163450

Purpose

lentiviral overexpression vector for human GLS

Depositor

Jason Cantor

Article

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GLS (GLS Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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SLC39A14-GFP

Plasmid

#104380

Purpose

Expresses SLC39A14/ZIP14 wild-type in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Article

Liu et al J Biol Chem. 2017 Oct 6;292(40):16605-16

Insert

SLC39A14 (SLC39A14 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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PKC gamma CAT

Plasmid

#21238

Depositor

Bernard Weinstein

Article

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC gamma (Prkcg Mouse)

Tags

HA

Expression

Mammalian

Mutation

delta 1-337

Available Since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

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