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pDONR221-MICU1-sh1-rORF

Plasmid

#31764

Depositor

Vamsi Mootha

Publication

Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

Insert

MICU1 (MICU1 Human)

Use

Pdonr gateway

Tags

None

Mutation

RNAi-resistant ORF (rORF). 8 synonymous changes i…

Promoter

None

Available since

May 4, 2012

Availability

Academic Institutions and Nonprofits only

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IGF2BP1

Plasmid

#155676

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

IGF2BP1 (IGF2BP1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pdCas9-GFP

Plasmid

#181906

Purpose

Fluorescently tagged dead Cas9

Depositor

Karsten Rippe

Publication

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9

Use

CRISPR

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEX-5X-1-Full-Length-MePCE

Plasmid

#113539

Purpose

Produces an N-terminal GST-tagged full length MePCE protein.

Depositor

Blerta Xhemalce

Publication

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-CAMKK2

Plasmid

#23422

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CAMKK2 (CAMKK2 Human)

Use

Gateway Cloning

Mutation

T253A

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-MPP5

Plasmid

#23447

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MPP5 (PALS1 Human)

Use

Gateway Cloning

Mutation

MMs?

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-PRKACB

Plasmid

#23787

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKACB (PRKACB Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-Tag2B Flag BimS

Plasmid

#23283

Depositor

Roger Davis

Publication

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

pCMV Tag2b Bim S (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pKan-PCUP1-9myc-AID*(N)

Plasmid

#99527

Purpose

N-terminal 9myc-AID* degron cassette with CUP1 promoter and KanR marker for S. cerevisiae

Depositor

Helle Ulrich

Publication

Morawska et al Yeast. 2013 Sep;30(9):341-51. doi: 10.10

Insert

IAA17(71-117) (AXR3 Mustard Weed)

Use

Pcr template for yeast tagging cassette

Tags

9xMyc

Promoter

CUP1

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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Klf2-t2A-mCherry

Plasmid

#127539

Purpose

Expresses mouse Klf2 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Publication

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Klf2 (Klf2 Mouse)

Expression

Mammalian

Promoter

CMV

Available since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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GST-AR-624-919

Plasmid

#104200

Purpose

Expresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion protein

Depositor

Elizabeth Wilson

Publication

He et al Mol Cell Biol. 2003 Mar;23(6):2135-50.

Insert

human androgen receptor aa 624-919 (AR Human)

Tags

GST

Expression

Bacterial

Available since

Dec. 6, 2017

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Vinculin-23

Plasmid

#55334

Purpose

Localization: Focal Adhesions, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Publication

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Vinculin (VCL Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

V234L in Vinculin.

Promoter

CMV

Available since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
peCALWY-3

Plasmid

#22235

Depositor

Maarten Merkx

Publication

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-3 Zn(II) sensor (ATOX1 GFP's, Human)

Expression

Mammalian

Mutation

Similar to eCALWY-1, but with a linkerlength of 3…

Available since

Nov. 20, 2009

Availability

Academic Institutions and Nonprofits only
MCP-NMS

Plasmid

#210705

Purpose

This plasmid express EF1a promoter driven MCP-NMS transactivation module

Depositor

Isaac Hilton

Publication

Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

Insert

MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1 (STAT1, MRTFA Synthetic, Human, bacteriophage MS2)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

N55K in MCP

Promoter

EF1a

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pPSU100

Plasmid

#173835

Purpose

Expresses 100 kD Penn State ladder protein

Depositor

Song Tan

Publication

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

STRHSTPABPACIL1bQRS

Expression

Bacterial

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 sgNRF2-3

Plasmid

#186838

Purpose

knock out NRF2 in mammalian cells

Depositor

Boyi Gan

Publication

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)

Use

CRISPR and Lentiviral

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmTurquoise2-LC3

Plasmid

#110947

Purpose

Expresses the autophagosome marker LC3 fused to mTurquoise2

Depositor

Pablo Rivera-Fuentes

Publication

Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

mTurquoise2

Expression

Mammalian

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

Plasmid

#101297

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Publication

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-906 of alpha-Catenin

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLX317-QKI

Plasmid

#115441

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

QKI (QKI Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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mOPN-pcDNA

Plasmid

#106447

Purpose

Mammalian expression of murine osteopontin

Depositor

Georg Weber

Publication

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

osteopontin (Spp1 Mouse)

Expression

Mammalian

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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