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pXPR_053

Plasmid

#113591

Purpose

Constitutive expression of a single sgRNA and the fluorophore violet-excited GFP in immune cells

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Commun. 2019 Apr 10;10(1):1668. doi:

Insert

sgRNA cloning site

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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3xFLAG-SENP7

Plasmid

#42886

Depositor

Edward Yeh

Article

Bawa-Khalfe et al Proc Natl Acad Sci U S A. 2012 Oct 23;10

Insert

SENP7 (SENP7 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

T102A, R969C, L996S

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

Plasmid

#62897

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pARF1-CFP

Plasmid

#11381

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Tags

CFP

Expression

Mammalian

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro hTERT shRNA

Plasmid

#10688

Depositor

William Hahn

Article

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

hTERT shRNA (TERT Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pmiHiFiCas9

Plasmid

#164832

Purpose

miHiFiCas9 expressing plasmid

Depositor

Eugene Chen

Article

Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

Insert

miHiFiCas9 (contains Brex27 at sequence 5031-5138).

Use

CRISPR

Tags

FLAG and NLS

Expression

Mammalian

Mutation

change R 691 to A

Promoter

CMV

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHR- flag-Lag16-Ecad

Plasmid

#205197

Purpose

This vector encodes of the synCAM (Ecad) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM Ecad and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pILGFPH3

Plasmid

#83553

Purpose

Used to evaluate the expression output of HXT7 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

HXT7 promoter-yEGFP

Expression

Yeast

Promoter

HXT7

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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CRY2olig-GP

Plasmid

#218918

Purpose

Expression of CRY2olig-mCherry fused to a codon-optimised poly-GP sequence

Depositor

Tatyana Shelkovnikova

Article

Hodgson et al iScience. 2024 Sep 14;27(10):110937. doi

Insert

CRY2olig-mCherry tagged poly-GP

Expression

Mammalian

Mutation

wild-type

Promoter

CMV

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pARF1(T31N)-CFP

Plasmid

#11384

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Tags

CFP

Expression

Mammalian

Mutation

Changed Threonine 31 to Asparagine.

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pILGFPI7

Plasmid

#83555

Purpose

Used to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

CUP1 promoter-yEGFP

Expression

Yeast

Promoter

CUP1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hFGD5

Plasmid

#50760

Purpose

expression of EGFP-tagged human FGD5

Depositor

Naoki Mochizuki

Article

Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

Insert

FGD5 (FGD5 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pET-28c(+)-YTHDF3

Plasmid

#102276

Purpose

For bacterial expression of His-tagged human YTHDF3

Depositor

Samie Jaffrey

Article

Patil et al Nature. 2016 Sep 7;537(7620):369-373. do

Insert

YTHDF3 (YTHDF3 Human)

Tags

6X HIS

Expression

Bacterial

Promoter

T7

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Lin28A

Plasmid

#51371

Depositor

Narry Kim

Article

Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

Insert

Lin28A (LIN28A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NES-fRCaMP2

Plasmid

#125250

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP2

Expression

Mammalian

Mutation

D396A

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-p65AD-Hsp70

Plasmid

#62914

Purpose

Creating p65AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-p65AD-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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JT113-pETDuet1-(R)-hREV3L

Plasmid

#64872

Purpose

bacterial expression of human REV3L

Depositor

Richard Wood

Article

Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

Insert

REV3L (REV3L Human)

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5 bovine GRK2

Plasmid

#14691

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 (GRK2 Bovine)

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
EGFP-Rab4a-7

Plasmid

#56440

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab4a (RAB4A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pAC8-DDIT4

Plasmid

#187116

Purpose

insect cell expression of DDIT4

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

DDIT4 (DDIT4 Human)

Tags

Strep

Expression

Insect

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Akt-AH

Plasmid

#39533

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt (PH domain only) (Akt1 Mouse)

Tags

6G linker and EGFP

Expression

Mammalian

Mutation

PH domain only

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
AA177

Plasmid

#215983

Purpose

Fragmid fragment: (N' terminus) Start codon only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Start

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AA178

Plasmid

#216046

Purpose

Fragmid fragment: (C' terminus) Stop only

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

Stop

Use

Fragment

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFSW GAP43-jRGECO1a P2A Syp-Halo

Plasmid

#185565

Purpose

Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

Depositor

Edwin Chapman

Article

Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

Insert

GAP43-jRGECO1a P2A Syp-Halo

Use

Lentiviral

Tags

HaloTag

Available Since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FL-fibulin-3-V5-lentiviral-GFP

Plasmid

#29703

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only
pLV-HNF4A-FOXA2-FOXA3

Plasmid

#149724

Purpose

expressing three liver promoting transcription factors (HNF4A, FOXA2, FOXA3)

Depositor

Baisong Lu

Article

Lu et al Biomaterials. 2021 Feb;269:120668. doi:

Insert

HNF4A-FOXA2-FOXA3

Use

Lentiviral

Tags

P2A, T2A

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pARF6(T27N)-CFP

Plasmid

#11386

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Tags

CFP

Expression

Mammalian

Mutation

Changed Threonine 27 to Asparagine.

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFPB5A

Plasmid

#83556

Purpose

Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

GAL1 promoter-yEGFP

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pICE-EGFP-FLAG-Ku70siR-WT

Plasmid

#46961

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

human Ku70 (XRCC6 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV Tet

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL5.0 E-cadherin shRNA/mE-cadherin-mCherry

Plasmid

#101282

Purpose

Lentiviral expression of shRNA and mouse E-cadherin -mCherry

Depositor

Alpha Yap

Article

Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

Insert

E-cadherin

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
STOP-eGFP-ROSA26TV

Plasmid

#11739

Depositor

Klaus Rajewsky

Article

Sasaki et al Immunity. 2006 Jun . 24(6):729-39.

Insert

STOP-eGFP-ROSA26TV

Use

Cre/Lox; /stop

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-Merlin S518A

Plasmid

#84294

Purpose

Expression of Merlin with an alanine mutation at the Pak phosphorylation site

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S518A

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-syn-fRCaMP2

Plasmid

#125246

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRCaMP2

Expression

Mammalian

Mutation

D396A

Promoter

synapsin

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST26-USP47

Plasmid

#64326

Purpose

Mammalian expression and purification of USP47.

Depositor

Grigory Dianov

Article

Parsons et al Mol Cell. 2011 Mar 4;41(5):609-15. doi:

Insert

USP47 (USP47 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR223-BNRF1

Plasmid

#198580

Purpose

Gateway Entry Clone

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BNRF1 (BNRF1 Epstein-Barr virus)

Use

Gateway entry vector

Mutation

None (wildtype)

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pILGFP91

Plasmid

#83548

Purpose

Used to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

PGK1 promoter-yEGFP

Expression

Yeast

Promoter

PGK1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBG-Pak1 83-149

Plasmid

#12214

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Pak1 (PAK1 Human)

Tags

GST

Expression

Mammalian

Mutation

Dominant negative Pak1: autoinhibitory domain, aa…

Available Since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEP4-191084-gp160

Plasmid

#123234

Purpose

Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (191084 B7-19) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP.N1-HDAC6.DC (catalytic deficient)

Plasmid

#36189

Depositor

Tso-Pang Yao

Article

Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

Insert

HDAC6.DC (HDAC6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

H216/611A

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C3-hYAP2

Plasmid

#17844

Depositor

Marius Sudol

Article

Basu et al Mol Cell. 2003 Jan . 11(1):11-23.

Insert

YAP (YAP1 Human)

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
hGip-R

Plasmid

#14942

Depositor

Bernard Thorens

Article

Gremlich et al Diabetes. 1995 Oct . 44(10):1202-8.

Insert

Gip-R (GIPR Human)

Expression

Mammalian

Mutation

Short form of the receptor

Available Since

June 7, 2007

Availability

Academic Institutions and Nonprofits only
pEGFP-Merlin S518D

Plasmid

#84295

Purpose

Expression of Merlin with a phosphomimic (aspartic acid) mutation at the Pak phosphorylation site

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Merlin (NF2) (NF2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

S518D

Promoter

CMV

Available Since

Oct. 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-HA-UBE3B-IRES-Puro

Plasmid

#107345

Purpose

Expresses HA tagged UBE3B in mammalian cells.

Depositor

Robert Sobol

Article

Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

Insert

UBE3B (UBE3B Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pENTR223-BORF2

Plasmid

#195834

Purpose

Gateway Entry Clone of Epstein-Barr virus BORF2

Depositor

Eric Johannsen

Article

Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

Insert

BORF2

Use

Gateway entry vector

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
plko-shmfn2-mito-GFP-ER tethering

Plasmid

#160509

Purpose

Rescue mfn2 knockdown by tethering mitochondria and ER with GFP

Depositor

Qing Deng

Article

Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

Insert

mito-GFP-ER tethering

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR- flag-Lag16-JAM-B

Plasmid

#205200

Purpose

This vector encodes of the synCAM (JAM-B) and GFP nanobody LAG16

Depositor

Wendell Lim

Article

Stevens et al Nature. 2023 Feb;614(7946):144-152. doi:

Insert

synCAM JAM-B and Lag16 anti-GFP nanobody

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-KS-attB2-SA(0)-T2A-dVP16AD-Hsp70

Plasmid

#62905

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a asyn(41-140)

Plasmid

#240596

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 41-140 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a asyn(36-140)

Plasmid

#240595

Purpose

Overexpress truncated alpha-Synuclein protein

Depositor

Jennifer Lee

Article

McGlinchey et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

aSyn (SNCA Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 36-140 only

Promoter

T7

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pvimentin-emiRFP670

Plasmid

#136572

Purpose

Vimentin labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-emiRFP670

Tags

emiRFP670

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 22,946 results for: Tes