We narrowed to 27,292 results for: Tat

1. AID-C12*-n'Cas9-BE4max

   Plasmid

   #216730

   Purpose

   Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

   Insert

   AID-C12*-n'Cas9-BE4max (AICDA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-DIO-3xHA-hM3D(Gq)-P2A-ArgiNLS-oScarlet

   Plasmid

   #220614

   Purpose

   Cre-dependent co-expression of 3x HA-tagged excitatory hM3D(Gq) DREADD receptor, and a single-cell discriminating version of oScarlet as a reporter.

   Depositor

   Sam Golden

   Article

   Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

   Insert

   3x HA-hM3D(Gq)-P2A-AgiNLS-oScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   3x HA; ArgiNLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXS-IRES-BLAST flag-CAD M1601E

   Plasmid

   #169828

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation at reported dimerization interface of the DHOase domain

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   M1601E; TCCC -> AGTC silent mutations at nt527…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-IRES-BLAST flag-CAD S1406A

   Plasmid

   #169820

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S1406A; TCCC -> AGTC silent mutations at nt527…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMXS-IRES-BLAST flag-CAD S1859E

   Plasmid

   #169822

   Purpose

   Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

   Depositor

   Richard Possemato

   Article

   Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

   Insert

   CAD (CAD Human)
   

   Use

   Retroviral

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   S1859E; TCCC -> AGTC silent mutations at nt527…

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AID-C12*-dCas9-BE4max

   Plasmid

   #216731

   Purpose

   Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.

   Depositor

   Rahul Kohli

   Article

   Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

   Insert

   AID-C12*-dCas9-BE4max (AICDA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX304-BCL-XL-499R

   Plasmid

   #203574

   Purpose

   Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499) in mammalian cells.

   Depositor

   Abhishek Chakraborty

   Article

   Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

   Insert

   BCL2L1 (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5- tagged

   Expression

   Mammalian

   Mutation

   silent point mutations introduced to make it resi…

   Promoter

   CMV

   Available since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3_ATP10B-E993A

   Plasmid

   #203698

   Purpose

   expresses ATP10B with the pathogenic mutation E993A

   Depositor

   Peter Vangheluwe

   Article

   Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

   Insert

   ATP10B (ATP10B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E993A

   Promoter

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3_ATP10B-E210A

   Plasmid

   #203697

   Purpose

   expresses ATP10B with the catalytic mutation E210A

   Depositor

   Peter Vangheluwe

   Article

   Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

   Insert

   ATP10B (ATP10B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E210A

   Promoter

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3_ATP10B-D433N

    Plasmid

    #203696

    Purpose

    expresses ATP10B with the catalytic mutation D433N

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D433N

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3_ATP10B-V748L

    Plasmid

    #203702

    Purpose

    expresses ATP10B with the pathogenic mutation V748L

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V748L

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3_ATP10B-I1038T

    Plasmid

    #203700

    Purpose

    expresses ATP10B with the pathogenic mutation I1038T

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    I1038T

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3_ATP10B-R153X

    Plasmid

    #203701

    Purpose

    expresses ATP10B with the pathogenic mutation R153X

    Depositor

    Peter Vangheluwe

    Article

    Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

    Insert

    ATP10B (ATP10B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R153X

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pT2/shCol1a1/Scarlet_Seq1.3

    Plasmid

    #206137

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1 (Col1a1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)-R/G-ICUE

    Plasmid

    #181849

    Purpose

    ICUE cAMP sensor using sfGFP and mRuby2 as the FRET donor and acceptor.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    R/G-ICUE (RAPGEF3 Human)
    

    Use

    Tags

    mRuby2 and sfGFP

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-shDnd1-No5

    Plasmid

    #70066

    Purpose

    TRC lentiviral vector for shRNA against mouse Dnd1 #5

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    Dnd1 (Dnd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLuc-Optop38i-dsm

    Plasmid

    #89748

    Purpose

    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor MK3BD3-13F

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    Optop38i dark-state mutant
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    C450A mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMDC43-FIT2-FLL[157-159]AAA

    Plasmid

    #96994

    Purpose

    Express GFP-tagged mutated mouse FIT2 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Biotechnol J. 2016 Dec 17. doi: 10

    Insert

    MmFIT2 (Fitm2 Mouse)
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    Amino acid residues 157-159 were mutated (FLL[157…

    Promoter

    35S

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSico shMAP3K2 mouse

    Plasmid

    #85657

    Purpose

    expression of shRNA targeting mouse MAP3K2

    Depositor

    Julien Sage

    Article

    Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

    Insert

    shRNA targeting 3'UTR of Map3k2 (Map3k2 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. FLT1 gRNA (BRDN0001146782)

    Plasmid

    #75558

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FLT1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    FLT1 (FLT1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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