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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,439 results for: eng

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Showing: 3161 - 3180 of 14439 results

  2. pCOM-CD63-COM

    Plasmid
    #138350
    Purpose
    Expressing COM-CD63-COM fusion protein in mammalian cells
    Depositor
    Baisong Lu
    Publication
    Yao et al J Extracell Vesicles. 2021 Mar;10(5):e12
    Insert
    COM-CD63-COM fusion protein
    Use
    CRISPR and Lentiviral
    Tags
    COM is fused to the N and C termini of human CD63
    Expression
    Mammalian
    Available since
    March 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    138350-plasmid-map-sequence-id-359687

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  3. pCXLE-Sox2-17-T2A-Klf4-E2A-Myc

    Plasmid
    #210016
    Purpose
    episomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve reset
    Depositor
    Hans Schöler
    Publication
    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sox2-17-t2a-Klf4-e2a-cMyc (Klf4, Sox2 Mouse)
    Expression
    Mammalian
    Mutation
    Sox2-17 is engineered highly cooperative chimeric…
    Promoter
    CAG
    Available since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    210016-plasmid-map-sequence-id-418270

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  7. MCP-eN3x9

    Plasmid
    #210706
    Purpose
    This plasmid express EF1a promoter driven MCP-eN3x9 transactivation module
    Depositor
    Isaac Hilton
    Publication
    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4
    Insert
    MCP fused to transactivation domain comprising eNRF2 and 3x 9aa Transactivation domain
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N55K in MCP
    Promoter
    EF1a
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    210706-plasmid-map-sequence-id-416266

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  8. pCBh_3x Flag-NLS-enRhCas12f1-NLS_pA_phU6_gRNA scaffold-spacer_pCMV_mCherry_pA

    Plasmid
    #197027
    Purpose
    Vector encoding a human codon-optimized enRhCas12f1 driven by CBh promoter, guide RNAs compatible with enRhCas12f1 driven by hU6, and mCherry driven by CMV promoter
    Depositor
    Xiangfeng Kong
    Publication
    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized enRhCas12f1
    Expression
    Mammalian
    Mutation
    L270R
    Promoter
    CBh, CMV, hU6
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    197027-plasmid-map-sequence-id-388964

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  9. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Publication
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

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  17. LentiCRISPRv2 RFP670

    Plasmid
    #187646
    Purpose
    lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site
    Depositor
    Martin Carroll
    Insert
    RFP 670
    Use
    Lentiviral
    Tags
    RFP670 and sgRNA cloning site
    Promoter
    EFS (P2A)
    Available since
    July 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187646-plasmid-map-sequence-id-367454

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  20. SuperClomeleon

    Plasmid
    #203724
    Purpose
    An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride
    Depositor
    George Augustine
    Publication
    Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cerulean linked topaz fluorescent protein
    Expression
    Bacterial and Mammalian
    Mutation
    Cerulean, modified Topaz Fluorescent Protein with…
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    203724-plasmid-map-sequence-id-403640

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Showing: 3161 - 3180 of 14439 results