2,293 results

1. pSynapsin_NLS_GFP_P2A_His6_NR1.

   Plasmid

   #170457

   Purpose

   Expresses histidine tagged NR1 in neuronal cells

   Depositor

   Shigeki Watanabe

   Article

   Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

   Insert

   NR1 (Grin1 Rat)
   

   Use

   Lentiviral

   Tags

   Hexa-histidin

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   July 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. hSyn1-sypHy-RGECO

   Plasmid

   #84078

   Purpose

   Combined fluorescent reporter for both presynaptic calcium influx and vesicle excoytosis

   Depositor

   Juan Burrone

   Article

   Jackson et al Front Synaptic Neurosci. 2016 Jul 26;8:2

   Insert

   sypHy-RGECO
   

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSET-RCaMP1h

   Plasmid

   #42874

   Purpose

   a single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRuby

   Depositor

   Loren Looger

   Article

   Akerboom et al Front Mol Neurosci. 2013;6:2. doi: 10.33

   Insert

   Red Fluorescent Calcium binding Protein
   

   Tags

   EK (Enterokinase) Recognition Site, His Tag (6x),…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-DIO-mGFP-MutCREB(S133A)

   Plasmid

   #194642

   Purpose

   Expresses the fusion of monomeric-EGFP-tagged mutant CREB(S133A) in a Cre-dependent manner

   Depositor

   Jun-Hyeong Cho

   Article

   Lee et al Nat Neurosci. 2022 Dec 23. doi: 10.1038/

   Insert

   CREB (Creb1 Rat)
   

   Use

   AAV

   Tags

   mEGFP

   Mutation

   S133A

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ankG-mCherry

   Plasmid

   #42566

   Depositor

   Benedicte Dargent

   Article

   Leterrier et al Proc Natl Acad Sci U S A. 2011 May 24;10

   Insert

   ankyrin G (Ank3 Rat)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 26, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV mKeima-LC3

   Plasmid

   #189006

   Purpose

   Fluorescent reporter for autophagy

   Depositor

   Atsushi Hoshino

   Article

   Minami et al Nat Commun. 2023 Jul 13;14(1):4084. doi:

   Insert

   mKeima, LC3 (Map1lc3b Rat)
   

   Use

   Retroviral

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-Synapsin-pHoenix-WPRE

   Plasmid

   #70111

   Purpose

   lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   pHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
   

   Use

   Lentiviral

   Tags

   Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…

   Promoter

   Synapsin

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. mOrange2-Lysosomes-20

   Plasmid

   #57968

   Purpose

   Localization: Lysosome Membrane, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Insert

   LAMP1
   

   Tags

   mOrange2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGP-AAV-syn-jGCaMP7s-WPRE

   Plasmid

   #104487

   Purpose

   AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP7s
   

   Use

   AAV

   Tags

   T7 epitope, Xpress tag, 6xHis

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmRFP_LAP2beta_IRES_puro2b

    Plasmid

    #21047

    Depositor

    Daniel Gerlich

    Article

    Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

    Insert

    LAP2_beta (TMPO Rat)
    

    Tags

    mRFP

    Expression

    Bacterial and Mammalian

    Available Since

    July 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-mTagBFP2-rMPC1 shRNA

    Plasmid

    #229016

    Purpose

    Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

    Depositor

    Ghazaleh Ashrafi

    Article

    Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

    Insert

    MPC1 (Mpc1 Rat)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-SEP_NR2B

    Plasmid

    #23998

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    NR2B (Grin2b Rat)
    

    Tags

    NR2B signal sequence (aa1-31) and SEP (supereclip…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFUGW ORANGE Gria1-GFP KI _ mCherry-KASH

    Plasmid

    #131507

    Purpose

    Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gria1, Gria1 Mouse, Rat)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hUBC

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p323-L10A-PATagRFP

    Plasmid

    #74172

    Purpose

    Fluorescent reporter for single ribosome tracking

    Depositor

    Robert Singer

    Article

    Katz et al Elife. 2016 Jan 13;5. pii: e10415. doi:

    Insert

    ribosomal protein L10A (Rpl10a Rat)
    

    Use

    Lentiviral

    Tags

    PATagRFP

    Promoter

    UbC

    Available Since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CAG.FLEX.iGluSnFR3.v857.GPI

    Plasmid

    #196218

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857 in GPI backbone. iGluSnFR3.v857.GPI

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV CAG.FLEX iGluSnFR3 v857.GPI
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    GPI Anchor, IgK-chain, and Myc epi tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-P2X2-YFP

    Plasmid

    #22400

    Depositor

    Henry Lester

    Article

    Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

    Insert

    ATP-gated P2X channel 2 (P2rx2 Rat)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Dec. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. Myo 9a-GFP

    Plasmid

    #134959

    Purpose

    Expression of rat Myo 9a (Myr7) in mammalian cells.

    Depositor

    Martin Bähler

    Article

    Chieregatti et al J Cell Sci. 1998 Dec 18;111 ( Pt 24):359

    Insert

    Myo 9a (rat) with 5' UTR (Myo9a Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV IE

    Available Since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGFP-Kif5c

    Plasmid

    #71853

    Purpose

    mammalian expression of GFP-Kif5c

    Depositor

    Michelle Peckham

    Article

    Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

    Insert

    Kif5c (Kif5c Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Kif5c starts at AA3

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid

    #218863

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8m
    

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBa. BDNF-Halo

    Plasmid

    #162718

    Purpose

    Mammalian expression of rat BDNF

    Depositor

    Marvin Bentley

    Article

    Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

    Insert

    Brain derived neurotrophic factor (Bdnf Rat)
    

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta actin

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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