We narrowed to 844 results for: nanobody

1. pJRK112

   Plasmid

   #173735

   Purpose

   donor plasmid for SapTrap assembly

   Depositor

   Mike Boxem

   Article

   Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

   Insert

   VHH(GFP)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET28a-nanoHER2-SpyTag003

   Plasmid

   #216312

   Purpose

   Expresses nanoHER2-SpyTag003 in bacterial cells. nanoHER2 is an anti-HER2 nanobody.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   nanoHER2-SpyTag003
   

   Use

   Tags

   His6 tag and SpyTag003

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. XXBLM (XXBLMA-c075)

   Plasmid

   #98223

   Purpose

   Vector for periplasmic expression of Lama antibodies with PelA leader and C-terminal His6 tag

   Depositor

   Nicola Burgess-Brown

   Insert

   XXBLMA (BLM Human)
   

   Use

   Tags

   His-6

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJRK254

   Plasmid

   #173742

   Purpose

   donor plasmid for SapTrap assembly

   Depositor

   Mike Boxem

   Article

   Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

   Insert

   VHH(mCherry)
   

   Use

   Other

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

   Plasmid

   #162878

   Purpose

   Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

   Depositor

   Frederic Meunier

   Article

   Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

   Insert

   Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)
   

   Use

   Tags

   mEos2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTXB1-nbOcIgG-DddA11_NT

   Plasmid

   #232441

   Purpose

   Anti-rabbit IgG Fc specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)

   Depositor

   Ivan Raimondi

   Article

   Chi et al bioRxiv [Preprint]. 2025 Jan 2:2024.12.3

   Insert

   nbOcIgG-DddA11_N
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTXB1-nbMmKappa-DddA11_NT

   Plasmid

   #232442

   Purpose

   Anti-mouse kappa chain specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)

   Depositor

   Ivan Raimondi

   Article

   Chi et al bioRxiv [Preprint]. 2025 Jan 2:2024.12.3

   Insert

   nbMmKappa-DddA11_NT
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-LambdaN-NbALFA

   Plasmid

   #207627

   Purpose

   Expresses LambdaN-NbALFA (ALFA-tag nanobody) fusion protein in mammalian cells (with a CMV promoter)

   Depositor

   Jay Shendure

   Article

   Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

   Insert

   LambdaN-NbALFA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   pCMV

   Available since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJYDC3

   Plasmid

   #162460

   Purpose

   Yeast display plasmid for expression of C-terminal fusions with Aga2p yeast agglutinin. The reporter DnbALFA was fused to the N-terminus of Aga2p.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Type

   Empty backbone

   Use

   Shuttle vector bacteria/yeast

   Tags

   DnbALFA designed ALFA nanobody, HA tag, HDEL sequ…

   Expression

   Mutation

   Promoter

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAWP78-PVCpnf_pvc13-antiHistoneNb

    Plasmid

    #198293

    Purpose

    Produces PVCs retargeted with A9 anti-histone nanobody

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiHistoneNb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-NbALFA-degFusionRed

    Plasmid

    #199466

    Purpose

    Expression of the photodegradable protein degFusionRed fused to an anti-ALFA-tag-specific nanobody in mammalian cells.

    Depositor

    Vladislav Verkhusha

    Article

    Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

    Insert

    NbALFA-degFusionRed
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFUSE-rabbitFc1-IL2ss-VHH(G08)-His-Myc

    Plasmid

    #209857

    Purpose

    Plasmid coding for a nanobody (termed G08) against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1

    Depositor

    Tim Brown

    Inserts

    IL2-ss
    VHH (G08 variant)
    Rabbit Fc Gamma 1
    

    Use

    Tags

    x3 MycTag and x6 HisTag

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1-HTLV promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHR_EF1a-->Gss52SR4-LaM4-P2A-NLSIFP

    Plasmid

    #162229

    Purpose

    Lentiviral vector for the expression of Gss52SR4-LaM4-P2A-NLSIFP in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2020 Oct 16;370(6514):327-331.

    Insert

    LaG17 GFP nanobody SynNotch tTA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMUT2-pCaf-mCherry

    Plasmid

    #192860

    Purpose

    pMUT2 based vector for sensing caffeine in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    Nanobody-CadC fusion protein and cognate promoter inducible by caffeine
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFUSE-rabbitFc1-IL2ss-VHH(B12)-His-Myc

    Plasmid

    #209837

    Purpose

    Plasmid coding for a nanobody against FGFR3 (mus musculus) with x6 HisTag, x3 myc, and Fabbit FC gamma 1

    Depositor

    Tim Brown

    Inserts

    IL2-ss
    VHH (B12 variant)
    Rabbit Fc Gamma 1
    

    Use

    Tags

    x3 MycTag and x6 HisTag

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1-HTLV promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTrcHis-7D12CTermTAG

    Plasmid

    #125267

    Purpose

    Expresses 7D12 anti-EGFR nanobodies with UAG codon at c-terminus for synthetic amino acid in combination with pEVOL-pAzF

    Depositor

    Angus Johnston

    Article

    Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

    Insert

    CTermTAG
    

    Use

    Tags

    His-Tag

    Expression

    Mutation

    Promoter

    Trc

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mAID(KR)–GFP NB(KR)

    Plasmid

    #232298

    Purpose

    KR GFP nanobody adaptor

    Depositor

    Masato Kanemaki

    Article

    Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GST-beta catenin tag

    Plasmid

    #186422

    Purpose

    Encodes GST fused to beta catenin tag recognized by anti-beta catenin nanobody

    Depositor

    Stephen Elledge

    Article

    Barber et al Cell Rep Methods. 2022 Oct 17;2(10):1003

    Insert

    GST-beta catenin tag
    

    Use

    Tags

    6His and GST-beta catenin tag (PDRKAAVSHWQQRS)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTrcHis-7D12Asp73TAG

    Plasmid

    #125266

    Purpose

    Expresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzF

    Depositor

    Angus Johnston

    Article

    Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

    Insert

    7D12 Asp73TAG
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTP396

    Plasmid

    #149336

    Purpose

    Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

    Depositor

    Rebecca Voorhees

    Article

    Pleiner et al Science. 2020 May 21. pii: science.abb50

    Insert

    anti-GFP nanobody
    

    Use

    Tags

    Biotin acceptor peptide (Avi-tag), His14, and SUM…

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHR_pGK_LaG17_synNotch_Gal4VP64

    Plasmid

    #79127

    Purpose

    Lentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)

    Depositor

    Wendell Lim

    Article

    Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

    Insert

    LaG17_synNotch_Gal4DBDVP64
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

    Plasmid

    #198450

    Purpose

    Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAWP78-PVCpnf_pvc13-antiMouseMHCIINb

    Plasmid

    #198292

    Purpose

    Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc13-antiMouseMHCIINb
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRPN265NB-LaG16-MNase

    Plasmid

    #170978

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Koidl et al Curr Protoc. 2021 Oct;1(10):e266. doi: 1

    Insert

    Glutathione-S-Transferase/LaG16/MNase
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHCMV-SS-antiMoonTagNb-TM

    Plasmid

    #198305

    Purpose

    Displays anti-MoonTag nanobody from the surface of mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pHCMV-SS-antiMoonTagNb-TM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTrcHIS-7D12Gln13TAG

    Plasmid

    #125264

    Purpose

    Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

    Depositor

    Angus Johnston

    Article

    Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

    Insert

    Gln13TAG 7D12
    

    Use

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHCMV-SS-antiALFAtagNb-TM

    Plasmid

    #198307

    Purpose

    Displays anti-ALFA nanobody from the surface of mammalian cells

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    pHCMV-SS-antiALFAtagNb-TM
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHEA

    Plasmid

    #168297

    Purpose

    Bacterial surface display of polypeptides (nanobodies) in E. coli

    Depositor

    Luis Ángel Fernández

    Article

    Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

    Insert

    Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
    

    Use

    Tags

    6xHis, E-tag, and PelB signal peptide

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pTREX GPR68_NB2_2

    Plasmid

    #127691

    Purpose

    Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    GPR68_NB2_2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pHRW-CRY2olig-VHH(GFP)

    Plasmid

    #126023

    Purpose

    express RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogenetics

    Depositor

    Eurico Morais-de-Sá

    Article

    Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

    Insert

    CRY2 PHRolig-VHH(GFP)
    

    Use

    Tags

    RFP

    Expression

    Insect

    Mutation

    CRY2 E490G

    Promoter

    Hsp70

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)

    Plasmid

    #165030

    Purpose

    Express cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHRW-CRY2-VHH(GFP)

    Plasmid

    #126022

    Purpose

    express RFP-tagged CRY2 PHR domain fused to anti-GFP nanobody for optogenetics

    Depositor

    Eurico Morais-de-Sá

    Article

    Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

    Insert

    CRY2 PHR-VHH(GFP)
    

    Use

    Tags

    RFP

    Expression

    Insect

    Mutation

    WT

    Promoter

    Hsp70

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. mAID(KR)–p53 NB(KR)

    Plasmid

    #232300

    Purpose

    KR p53 adaptor

    Depositor

    Masato Kanemaki

    Article

    Islam et al J Cell Sci. 2025 Jun 9:jcs.263961. doi:

    Inserts

    KR p53 nanobody adaptor
    OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. mCh-LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)

    Plasmid

    #165029

    Purpose

    Express cpFRB1-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherry

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaGFP(N)-cpFRB1-T2A-FKBP-LaGFP(C)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. GFP-LaM(N)-cpFRB1-T2A-FKBP-LaM(C)

    Plasmid

    #165026

    Purpose

    Express cpFRB1-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaM8(N)-cpFRB1-T2A-FKBP-LaM8(C)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. GFP-LaM(N)-cpFRB2-T2A-FKBP-LaM(C)

    Plasmid

    #165028

    Purpose

    Express cpFRB2-based mCherry specific Chessbody (chemically switchable split nanobody); visualized by GFP

    Depositor

    Yubin Zhou

    Article

    Lee et al J Mol Biol. 2020 May 1;432(10):3127-3136

    Insert

    LaM8(N)-cpFRB2-T2A-FKBP-LaM8(C)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3.1 5HT2A_NB2_1

    Plasmid

    #127690

    Purpose

    Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

    Depositor

    Justin English, Bryan Roth

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    5HT2A_NB2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET21b-LaG9(S151C)

    Plasmid

    #172490

    Purpose

    Encodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).

    Depositor

    Siegfried Musser

    Article

    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d

    Insert

    LaG9(S151C)
    

    Use

    Tags

    6xHis, PPX cleavage site, and pelB leader sequenc…

    Expression

    Bacterial

    Mutation

    S151C

    Promoter

    T7

    Available since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pHR Ef1a_LaG16-synNotch_tTA-PGK_Puro

    Plasmid

    #223536

    Purpose

    Anti-GFP LaG16 nanobody-based synNotch; puro-selectable

    Depositor

    Jonathan Brunger

    Article

    Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

    Insert

    LaG16_synNotch_tTA
    

    Use

    Lentiviral

    Tags

    c-myc tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRDA_886

    Plasmid

    #201162

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' VP64 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. APEX2-GBP

    Plasmid

    #67651

    Purpose

    Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody).

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    APEX2-GBP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. Nb-gp41-Halo (MoonTag-Nb-Halo)

    Plasmid

    #128603

    Purpose

    Expresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    Nanobody_gp41
    

    Use

    Lentiviral

    Tags

    GB1 and Halo

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Aug. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRDA_887

    Plasmid

    #201164

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pVDL9.3

    Plasmid

    #168299

    Purpose

    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA

    Depositor

    Víctor de Lorenzo

    Article

    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do

    Insert

    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    LacI-Plac

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. Nb-gp41-GFP (MoonTag-Nb-GFP)

    Plasmid

    #128602

    Purpose

    Expresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).

    Depositor

    Marvin Tanenbaum

    Article

    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

    Insert

    Nanobody_gp41
    

    Use

    Lentiviral

    Tags

    GB1 and sfGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pcDNA3-endo-Nb37

    Plasmid

    #205514

    Purpose

    Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-Nb37
    

    Use

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pcDNA3-nuc-Nb37

    Plasmid

    #205515

    Purpose

    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb37
    

    Use

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3-endo-Nb80

    Plasmid

    #205518

    Purpose

    Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    endo-Nb80
    

    Use

    Tags

    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pRDA_888

    Plasmid

    #201165

    Purpose

    Lentiviral expression of EnAsCas12a CRISPRa gRNA with N' nanobody-ALFA and C' p65, HSF1 and NLS

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA3-nuc-Nb80

    Plasmid

    #205519

    Purpose

    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb80
    

    Use

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge