We narrowed to 4,972 results for: puc
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Plasmid#64966Purposemini-Tn7 vector with oriR6K, for attTn7 site determinationDepositorInsertoriR6K
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2
Plasmid#98973PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 2 and Cas9(D10A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1977 (pUC19-PTDH3-PTEF1)
Plasmid#78230PurposeExample plasmid containing a bidirectional promoter of pTDH3 and pTEF1DepositorInsertBidirectional promoters pTDH3-pTEF1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19.TRIM28.FKBP.knock-in.mCerulean
Plasmid#185009PurposeFor knocking in FKBP F36V P2A mCeruleanDepositorInsertFKBP F36V P2A mCerulean
UseCloningTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-link-PAmCherry-FLAG:cat
Plasmid#211832PurposePlasmid template that encodes the fluorescent protein PAmCherry1 with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out PAmcherry1-FLAG-cat for genetic tagging.DepositorInsertPAmCherry-FLAG-cat
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19, containing H2-Kd Balb/c WK10
Plasmid#102642PurposeEncodes sequence of the murine H2-Kd alleleDepositorInsertH2-Kd Balb/c
UseTagsExpressionMutationPromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC-H1-Esp3I-mU6-Kan
Plasmid#213015PurposeVector for Cloning of multiple gRNAs driven by distinct promoters (H1 and mU6)DepositorInsertH1 promoter, mU6 promoter, gRNA scaffolds
UseTagsExpressionMutationPromoterH1 promoterAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(203)
Plasmid#44377DepositorInsertUAS1B16-TEF(203) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(203)Available sinceApril 16, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19-link-PAGFP-FLAG:Kan
Plasmid#211834PurposePlasmid template that encodes the fluorescent protein PAGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out PAGFP-FLAG-Kan for genetic tagging.DepositorInsertPAGFP-FLAG-Kan
UseTagsExpressionBacterialMutationNAPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-TSV (linearized in VP2)
Plasmid#70032PurposepUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding regionDepositorInsertTSV full genome, linearized in VP2 gene
UseCloning vectorTagsExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC57-GSX2-tTA-BleoR
Plasmid#161748PurposeTet-ON tTA plasmid for the c-terminal targeting of human GSX2 locusDepositorInsertAdvanced tTA
UseCRISPRTagsExpressionMammalianMutationPromoterendogenous GSX2 promoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB1975 (pUC19-PTEF1-PPGK1)
Plasmid#73296PurposeExample plasmid containing a bidirectional promoterDepositorInsertpTEF1-pPGK1
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC_h7SK Mma PylT (CUA)
Plasmid#154782Purposeplasmid with single copy of Mma PylT (CUA anticodon)DepositorInsertMma PylT
UseTagsExpressionMammalianMutationU25CPromoterh7SKAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1
Plasmid#98976PurposeCas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.DepositorInsertLMNA sgRNAs pair 1 and Cas9(N863A)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Top-Toehold-mCherry
Plasmid#87318PurposeExpresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.DepositorInsertSplit-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19 ACMV DNA-B 1.5mer
Plasmid#159135PurposeInfectious clone containing a partial-tandem-dimer of African cassava mosaic virus CM:2:98 DNA-BDepositorInsertACMV DNA-B 1.5mer
UseInfectious clone for bombarding plantsTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(1004)
Plasmid#44365DepositorInsertUAS1B16-TEF(1004) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-TEF(1004)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC_h7SK Mma PylT (UUA)
Plasmid#154783Purposeplasmid with single copy of Mma PylT (UUA anticodon)DepositorInsertMma PylT
UseTagsExpressionMammalianMutationU25C and UUA anticodonPromoterh7SKAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Sa sgRNA expression vector
Plasmid#107720PurposeFor in vitro transcription of Sa sgRNA from the T7 promoter.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-Top-T~Bottom-T
Plasmid#87315PurposeControl plasmid. Encodes the Split-Broccoli system (Top + Bottom) with only "Top" under the control of E. coli sigma 70 promoter.DepositorInsertSplit-Broccoli system (promoter control)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-LacZ30
Plasmid#65028PurposeGmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 3. See Depositor Comments below for information regarding the lacZ sequence.DepositorTypeEmpty backboneUseTagslacZExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19 gene 60 delta gap
Plasmid#49091PurposeTemplate for generating gene 60 mRNA, coding gap deletionDepositorInsertBacteriophage T4 gene 60
UseTagsExpressionMutationCoding gap and coding gap STOP have been deleted.PromoterT7Available sinceJan. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC 3GLA UAS HAi
Plasmid#71763Purposegap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tagDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(1004)
Plasmid#44366DepositorInsertUAS1B8-TEF(1004) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(1004)Available sinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
Mishler2014-No-aptamer-controlluciferase-pUC19
Plasmid#63856PurposeThis pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.DepositorInsertNo-aptamer control mRNA
UseSynthetic BiologyTagsExpressionMutationPromotertacAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF(203)
Plasmid#44378DepositorInsertUAS1B8-TEF(203) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(203)Available sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-UAS1B8-TEF(804)
Plasmid#44368DepositorInsertUAS1B8-TEF(804) promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEF(804)Available sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19::ZmUBI-RWT4Ca_K217R/K327R
Plasmid#237511PurposeProtoplast expressionDepositorInsertRwt4Ca_K217R/K327R
UseTagsExpressionPlantMutationMutations at kinase active sites (K217R, K327R)PromoterAvailable sinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-FlpTag-GFP-ph0
Plasmid#231897PurposeVector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 0.DepositorInsertSD-GFP-SA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-FlpTag-GFP-ph1
Plasmid#231898PurposeVector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 1.DepositorInsertSD-GFP-SA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC57-FlpTag-GFP-ph2
Plasmid#231899PurposeVector with FlpTag-GFP, an inverted GFP enabling conditional tagging and visualization of endogenous proteins via Flp recombinase activity. Codon phase 2.DepositorInsertSD-GFP-SA
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19_msfGFP-HP1a-repair-template
Plasmid#238248PurposeFor knock-in msfGFP to HP1a locusDepositorInsertHP1a
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19_msfGFP-TCOF1-repair-template
Plasmid#237632PurposeFor knock-in msfGFP to TCOF1 locusDepositorInsertTCOF1 (TCOF1 Human)
UseCRISPRTagsmsfGFPExpressionMutationPromoterAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19_msfGFP-FUS-repair-tempate
Plasmid#237684PurposeFor knock-in msfGFP to FUS locusDepositorInsertFUS (FUS Human)
UseCRISPRTagsmsfGFPExpressionMutationPromoterAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19::ZmUBI-RWT4Ca+C-Tail
Plasmid#237514PurposeProtoplast expressionDepositorInsertRwt4Ca+C-Tail
UseTagsExpressionPlantMutationAdd C-terminal extensionPromoterAvailable sinceApril 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-RELA-PGK-EGFP
Plasmid#228051PurposeHomologous donor template for RELA knockout expressing EGFPDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-RELB-PGK-EGFP
Plasmid#228052PurposeHomologous donor template for RELB knockout expressing EGFPDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-RELC-PGK-EGFP
Plasmid#228053PurposeHomologous donor template for RELC knockout expressing EGFPDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-RELA-PGK-tdTomato
Plasmid#228054PurposeHomologous donor template for RELA knockout expressing tdTomatoDepositorInserttdTomato
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-RELC-PGK-tdTomato
Plasmid#228056PurposeHomologous donor template for RELC knockout expressing tdTomatoDepositorInserttdTomato
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crScaffold_SV40-BFP
Plasmid#224861PurposecrRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoterDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crScaffold_EF1a-BFP
Plasmid#224862PurposecrRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoterDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crBFP_EF1a-BFP
Plasmid#224783PurposeBFP-targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and target BFP protein expressed from EF1a promoterDepositorInsertcrBFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crEGFP_EF1a-BFP
Plasmid#224784PurposeEGFP targeting crRNA for RfxCas13d expressed from hU6-2xTetO promoter and non-target BFP protein expressed from EF1a promoterDepositorInsertcrEGFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-crBFP_EF1a-BFP
Plasmid#224785PurposeBFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoterDepositorInsertcrBFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6 and hU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-CrEGFP_EF1a-BFP
Plasmid#224786PurposeEGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoterDepositorInsertcrBFP
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6 and hU6-2xTetOAvailable sinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-FLAG-HA-Regnase-3 WT
Plasmid#222651PurposeFor in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-termDepositorInsertZc3h12c (Zc3h12c Mouse)
UseIn vitro transcriptionTagsFLAG and HAExpressionMutationPromoterT7Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-NFKB1-PGK-EGFP
Plasmid#204018PurposeHomologous donor template for NFKB1 knockout expressing EGFPDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-NFKB1-PGK-tdTomato
Plasmid#204019PurposeHomologous donor template for NFKB1 knockout expressing tdTomatoDepositorInserttdTomato
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-NFKB2-PGK-EGFP
Plasmid#204020PurposeHomologous donor template for NFKB2 knockout expressing EGFPDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterPGKAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only