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Showing: 751 - 800 of 4896 results

  4. pUC19-Top-Toehold-mCherry

    Plasmid
    #87318
    Purpose
    Expresses the "Top" transcript of the Split-Broccoli system upstream of an RNA Toehold switch controlling mCherry.
    Depositor
    Donald Burke
    Article
    Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102
    Insert
    Split-Broccoli "Top" transcript upstream of an RNA Toehold (TS2_KS01) controlling mCherry translation.
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P70a
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    87318-plasmid-map-sequence-id-174668

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  10. pUC18-mini-Tn7T-Gm-LacZ30

    Plasmid
    #65028
    Purpose
    GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 3. See Depositor Comments below for information regarding the lacZ sequence.
    Depositor
    Herbert Schweizer
    Article
    Choi et al Nat Protoc. 2006;1(1):153-61.
    Type
    Empty backbone
    Use
    Tags
    lacZ
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    65028-plasmid-map-sequence-id-109988

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  13. Mishler2014-No-aptamer-controlluciferase-pUC19

    Plasmid
    #63856
    Purpose
    This pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.
    Depositor
    Howard Salis
    Article
    Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv
    Insert
    No-aptamer control mRNA
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    tac
    Available since
    Feb. 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pUC19_msfGFP-FUS-repair-tempate

    Plasmid
    #237684
    Purpose
    For knock-in msfGFP to FUS locus
    Depositor
    Denes Hnisz
    Insert
    FUS (FUS Human)
    Use
    CRISPR
    Tags
    msfGFP
    Expression
    Mutation
    Promoter
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    237684-plasmid-map-sequence-id-471688

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  20. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid
    #228253
    Purpose
    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    228253-plasmid-map-sequence-id-451024

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  38. pUC-T7-hTP53-P0~670-FLuc

    Plasmid
    #208640
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.
    Depositor
    Quanfu Ma
    Insert
    TP53, UTR and partial CDS (TP53 Human)
    Use
    Luciferase; In vitor transcription template
    Tags
    Firefly Luciferase (F-Luc) and T7 promoter
    Expression
    Mutation
    UTR and partial CDS in-frame with F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208640-plasmid-map-sequence-id-418347

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  39. pUC-ODN-hsRPL3-CterTwStrep

    Plasmid
    #208644
    Purpose
    for long single strand DNA donnor preparation for Twin Strep tag knock-in at C ter of human RPL3. Digest with nt.BspQI and nb.BsrDI to get ssDNA. This plasmid will cause re-edit and fail knock-in.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Synthetic, Human)
    Use
    CRISPR; Ssdna donnor preparation
    Tags
    Twin Strep
    Expression
    Mutation
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208644-plasmid-map-sequence-id-418358

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  45. pUC19 KGA HA5'-mKO2-3'

    Plasmid
    #110406
    Purpose
    Homology donor for glutaminase KGA isoform knock-in, mKO2
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none
    Available since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110406-plasmid-map-sequence-id-218847

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Showing: 751 - 800 of 4896 results