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pR-spondin 1

Plasmid

#128844

Purpose

bacterial expression of pR-spondin 1

Depositor

Marc de la Roche

Article

Urbischek et al Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.

Insert

R-spondin 1 (RSPO1 Human)

Expression

Bacterial

Promoter

T7

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCGN-Oct-1

Plasmid

#53308

Purpose

plasmid contains POU2F1 (Oct-1) gene with HA-tag

Depositor

Winship Herr

Article

Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Insert

Oct-1 (POU2F1 Human)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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pCG-Oct-1

Plasmid

#53306

Purpose

plasmid contains POU2F1 (Oct-1) gene

Depositor

Winship Herr

Article

Tanaka et al Cell. 1990 Feb 9;60(3):375-86.

Insert

Oct-1 (POU2F1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only

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pSuper 44-1

Plasmid

#51597

Purpose

shRNA 1 against rat and mouse Sema4D

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

shRNA1

Use

RNAi

Promoter

U6

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pPtet-1-VP16

Plasmid

#213843

Purpose

Used for expressing VP16 fused to ZF-1.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

VP16

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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Rabbit NHERF-1

Plasmid

#31644

Depositor

Edward Weinman

Article

Weinman et al J Clin Invest. 1995 May . 95(5):2143-9.

Insert

solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1 (SLC9A3R1 Rabbit)

Expression

Bacterial

Mutation

none

Available Since

Feb. 14, 2012

Availability

Academic Institutions and Nonprofits only
pX459-homo_PTGS1-1

Plasmid

#184731

Purpose

COX1-KO in HeLa

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the human COX1 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2ALexA

Plasmid

#125221

Purpose

Artificial phase 1 exon to include a spliced T2A-LexA effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

LexA

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-shTRAF2#1

Plasmid

#44127

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral and RNAi

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pBS_KS_attB2_SA(1)_T2AGAL4

Plasmid

#125212

Purpose

Artificial phase 1 exon to include a spliced T2A-GAL4 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GAL4

Use

Other

Tags

T2A

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pBluescript JTV-1

Plasmid

#16473

Depositor

Bert Vogelstein

Article

Nicolaides et al Genomics. 1995 Sep 20. 29(2):329-34.

Insert

JTV-1 (AIMP2 Human)

Expression

Bacterial

Available Since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pX459-homo_PLA2G4A-1

Plasmid

#184735

Purpose

cPLA2-KO in HeLa

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

A gRNA targeting the human cPLA2 gene.

Use

CRISPR

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBS_KS_attB2_SA(1)_T2AGeneSwitch

Plasmid

#125215

Purpose

Artificial phase 1 exon to include a spliced T2A-GeneSwitch effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a gene

Depositor

Herman Dierick

Article

Gnerer et al Nucleic Acids Res. 2015 Apr 30;43(8):e56

Insert

GeneSwitch

Use

Other

Tags

T2A

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRS314-prp16-1

Plasmid

#89972

Purpose

prp16-1 allele in TRP plasmid

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP16 (PRP16 Budding Yeast)

Expression

Yeast

Mutation

prp16-1 allele (Y386D)

Availability

Academic Institutions and Nonprofits only

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pLKO.1-blast

Plasmid

#26655

Purpose

3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses blasticidin for selection.

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-1:ChrA

Plasmid

#225079

Purpose

Expresses precursor peptide ChrA in E.coli

Depositor

Wilfred van der Donk

Article

Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

Insert

ChrA

Tags

His6

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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EFF-1::V5

Plasmid

#132961

Purpose

Expresses EFF-1 A full length protein in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Valansi et al J Cell Biol. 2017 Mar 6;216(3):571-581.

Insert

EFF-1::V5

Tags

V5

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Lsg-LRRK2-1

Plasmid

#199277

Purpose

nicking sgRNA to induce LRRK2 (c. 6055 G > A) mutation using PE3

Depositor

Ting Zhou

Article

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Insert

LRRK2 nick

Expression

Mammalian

Available Since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPRa-Theo-1

Plasmid

#240207

Purpose

Bacterial CRISPRa vector expressing dCas9, MCP-SoxS, and a theophylline-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

dCas9
MCP-SoxS
Theophylline MR-scRNA variant 1

Use

CRISPR

Expression

Bacterial

Promoter

J23107, J23119, and Sp.pCas9

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSpRYa-Theo-1

Plasmid

#240208

Purpose

Bacterial SpRYa vector expressing dSpRY, MCP-SoxS, and a theophylline-responsive MR-scRNA

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Inserts

dSpRY
MCP-SoxS
Theophylline MR-scRNA variant 1

Use

CRISPR

Expression

Bacterial

Promoter

J23107, J23119, and Sp.pCas9

Available Since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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