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pCMV_mEm_4GS_LF82_105

Plasmid

#100310

Purpose

Mammalian expression of E. coli LF82_105 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_105

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_mEm_4GS_LF82_106

Plasmid

#100311

Purpose

Mammalian expression of E. coli LF82_106 CDS fused to mEmerald

Depositor

Alan Huett

Article

Collins et al Sci Data. 2018 May 15;5:180081. doi: 10.

Insert

LF82_106

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pWT019a

Plasmid

#107893

Purpose

W1.1 plasmid. Contains PTetO-sd2U-Cas9, PLacO-sgRNA1 and is part of CAMERA 1.1

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2U-Cas9, PLacO-sgRNA1

Expression

Bacterial

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pHYRSF1-02

Plasmid

#24765

Depositor

Hideo Iwai

Article

Muona et al Nat Protoc. 2010 . 5(3):574-87.

Insert

NpuDnaE-IntC

Tags

GB1 and His

Expression

Bacterial

Available Since

June 18, 2010

Availability

Academic Institutions and Nonprofits only

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pWT021a

Plasmid

#107895

Purpose

W2.0 plasmid. Contains PTetO-SD8-BE2, PLac-sgRNA1 and is part of CAMERA 2.0

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PLac-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTK565

Plasmid

#114690

Purpose

SNAP-tagging at DHC's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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pWT037a

Plasmid

#107896

Purpose

W2.1 plasmid. Contains PTetO-SD8-BE2, PLacO-sgRNA1 and is part of CAMERA 2.1

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PLacO-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT050a

Plasmid

#107899

Purpose

W2.4-2 plasmid. Contains PLacO-sgRNA1, PRha-sgRNA4 and is part of CAMERA 2.4

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PLacO-sgRNA1, PRha-sgRNA4

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT063a

Plasmid

#107908

Purpose

W2m.1-1 plasmid. Contains TRESG-BE3 and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

TRESG-BE3

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pWT046b

Plasmid

#107898

Purpose

W2.3 plasmid. Contains PTetO-SD8-BE2, PRha-sgRNA4 and is part of CAMERA 2.3

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PRha-sgRNA4

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT007f

Plasmid

#107891

Purpose

W1.0.3 plasmid. Contains PTetO-sd2U-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.3

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2U-Cas9, PLac-sgRNA1

Expression

Bacterial

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pWT062a

Plasmid

#107909

Purpose

W2m.2-2 plasmid. Contains UBC-TetR-P2A-LacI and is ppart of the CAMERA2m system for cellular recording in mammalian cells

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

UBC-TetR-P2A-LacI

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6 - iFAST

Plasmid

#209414

Purpose

iFAST containing plasmid for endogenous fusion constructs

Depositor

Patrick Van Dijck

Article

Devos et al Microbiology (Reading). 2024 Mar;170(3).

Insert

iFAST

Expression

Yeast

Promoter

none

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pWT007a

Plasmid

#107889

Purpose

W1.0.1 plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.1

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-Cas9, PLac-sgRNA1

Expression

Bacterial

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTK583

Plasmid

#114695

Purpose

SNAP-tagging at NuMA's C-terminal locus

Depositor

Tomomi Kiyomitsu

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Insert

SNAP

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pWT047c

Plasmid

#107903

Purpose

W2.7-1 plasmid. Contains PR-sd5-BE2, PLac-sgRNA1 and is part of CAMERA 2.7

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PR-sd5-BE2, PLac-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT045c

Plasmid

#107902

Purpose

W2.6 plasmid. Contains PTetO-SD8-BE2, PPSP-sgRNA1 and is part of CAMERA 2.6

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PPSP-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pWT007g

Plasmid

#107890

Purpose

W1.0.2 plasmid. Contains PTetO-sd2-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.2

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-sd2-Cas9, PLac-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pT7RNAP

Plasmid

#170101

Purpose

expresses T7 RNAP

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

T7-RNA polymerase

Use

Synthetic Biology

Expression

Bacterial

Promoter

p70a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pWJ 1781 URA

Plasmid

#245252

Purpose

Protein expression in yeast

Depositor

Neel Shah

Article

Jiang et al Nat Commun. 2025 Jul 1;16(1):5464. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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