Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 9,393 results for: Coli

Showing: 6401 - 6440 of 9393 results

  1. pPD1R

    Plasmid
    #86969
    Purpose
    Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH
    Promoter
    T7 promoter
    Available since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    86969-plasmid-map-sequence-id-173876

    Enlarge

  2. pPD2cX

    Plasmid
    #86968
    Purpose
    Expresses PCNA2 variant (L171C) fused phosphite dehydrogenase and putidaredoxin in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA2 variant (L171C) fused to phosphite dehydrogenase and putidaredoxin
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, C73S/C85S in PdX, L171C in P…
    Promoter
    T7 promoter
    Available since
    March 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    86968-plasmid-map-sequence-id-173869

    Enlarge

  4. 36INS2-pBAD_GB

    Plasmid
    #78677
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [G:36INS2-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    78677-plasmid-map-sequence-id-147244

    Enlarge

  5. 36INS2-pBAD_FB

    Plasmid
    #78676
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [F:36INS2-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78676-plasmid-map-sequence-id-147245

    Enlarge

  6. 36INS2-pBAD_EB

    Plasmid
    #78675
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [E:36INS2-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78675-plasmid-map-sequence-id-147246

    Enlarge

  7. 36INS2-pBAD_AB

    Plasmid
    #78674
    Purpose
    Insulated MoClo promoter for E. coli. 36nt DNA spacer empirically selected. Modified from BBa_I13453. Has near identical expression as 36INS1-pBAD_AB [A:36INS2-pBAD:B]
    Depositor
    Douglas Densmore
    Article
    Carr et al PLoS One. 2017 Apr 19;12(4):e0176013. do
    Insert
    Insulated pBAD Promoter
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    78674-plasmid-map-sequence-id-147247

    Enlarge

  10. pfMDH_K381A

    Plasmid
    #67732
    Purpose
    Expresses K381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed lysine 381 to alanine
    Promoter
    T7
    Available since
    Sept. 11, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pfMDH_N300D

    Plasmid
    #67728
    Purpose
    Expresses N300D mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 300 to aspartate
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pfMDH_R373A

    Plasmid
    #67731
    Purpose
    Expresses R373A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed arginine 373 to alanine
    Promoter
    T7
    Available since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  13. pfMDH_N300S

    Plasmid
    #67729
    Purpose
    Expresses N300S mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed asparagine 300 to serine
    Promoter
    T7
    Available since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pUNC2001

    Plasmid
    #67280
    Purpose
    Expresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcw
    Depositor
    Aziz Sancar
    Article
    Worthington et al J Biol Chem. 2003 Oct 3;278(40):39143-54
    Insert
    Vibrio cholera photolyase
    Use
    Tags
    maltose binding protein
    Expression
    Bacterial
    Mutation
    none known
    Promoter
    Available since
    July 23, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pAN-OR-MalT-3NT

    Plasmid
    #62309
    Purpose
    synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome
    Depositor
    Christopher Voigt
    Article
    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:
    Inserts
    A2NT sgRNA
    Malt-3NT sgRNA
    A2NT sgRNA
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PA2, PPhlF, and pBAD
    Available since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    62309-plasmid-map-sequence-id-102224

    Enlarge

  19. SNV-lacZ

    Plasmid
    #53125
    Purpose
    retroviral vector expressing lacZ, driven by the Spleen Necrosis Virus promoter; for use in chicken cells
    Depositor
    Jeanette Hyer
    Article
    Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1
    Insert
    lacZ (lacZ E.coli)
    Use
    Retroviral; Expression in chicken cells
    Tags
    Expression
    Mutation
    Promoter
    SNV (spleen necrosis virus)
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pHL1757

    Plasmid
    #53037
    Purpose
    PconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetR
    Depositor
    Han Lim
    Article
    Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110
    Inserts
    GFP
    tetR
    Use
    Synthetic Biology
    Tags
    RBS(st3) and glgC leader
    Expression
    Bacterial
    Mutation
    Promoter
    PconNoHindM2 and pConNoHind
    Available since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  23. pEVOL-pBpF

    Plasmid
    #31190
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.
    Depositor
    Peter Schultz
    Article
    Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99
    Insert
    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G, E107P, D158T, I159S
    Promoter
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31190-plasmid-map-sequence-id-418765

    Enlarge

  26. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid
    #198468
    Purpose
    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.
    Depositor
    Viviana Risca
    Article
    Soroczynski et al bioRxiv 2024.07.11.602973
    Insert
    10His-pG-Tn5 (E54K, L372P)
    Use
    Tags
    10His-pG
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    Consensus φ10 T7 Promoter
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198468-plasmid-map-sequence-id-390256

    Enlarge

  27. pCasSA

    Plasmid
    #98211
    Purpose
    CRISPR-based E. coli/Staphylococcus aureus temperature-sensitive plasmid for genome editing in Staphylococcus aureus using S. pyogenes Cas9
    Depositor
    Quanjiang Ji
    Article
    Chen et al J Am Chem Soc. 2017 Mar 15;139(10):3790-
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    98211-plasmid-map-sequence-id-485072

    Enlarge

  39. pET-MCN-10His-mStayGold (E138D)

    Plasmid
    #211361
    Purpose
    Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.
    Depositor
    Mohan Balasubramanian
    Article
    Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103
    Insert
    mStayGold
    Use
    Tags
    10xHis-tag
    Expression
    Bacterial
    Mutation
    E138D
    Promoter
    T7
    Available since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    211361-plasmid-map-sequence-id-417678

    Enlarge

  40. pTriEx4-Cdc42-2G

    Plasmid
    #68814
    Purpose
    FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)
    Depositor
    Olivier Pertz
    Article
    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10
    Insert
    Cdc42 activation reporter
    Use
    Tags
    His and Myc
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    CMV
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    68814-plasmid-map-sequence-id-120972

    Enlarge

Showing: 6401 - 6440 of 9393 results