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Plasmid#229437Purposeknockout of MARK3DepositorInsertsgMARK3-R-1 (MARK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-R-3
Plasmid#229436Purposeknockout of MARK2DepositorInsertsgMARK2-R-3 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-R-2
Plasmid#229435Purposeknockout of MARK2DepositorInsertsgMARK2-R-2 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
sgMARK2-R-1
Plasmid#229434Purposeknockout of MARK2DepositorInsertsgMARK2-R-1 (MARK2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)
Plasmid#226989PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2bDepositorInsertSpCas9 gRNA 1a to create OPA1-del_exon5
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)
Plasmid#226991PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1bDepositorInsertSpCas9 gRNA 2a to create OPA1-del_exon5
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A6-gRNA_(CJT88)
Plasmid#226987PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A6)DepositorInsertSpCas9 gRNA A6 to create OPA1-R194G
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A7-gRNA_(CJT89)
Plasmid#226986PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A7)DepositorInsertSpCas9 gRNA A7 to create OPA1-R194G
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)
Plasmid#226992PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1bDepositorInsertSpCas9 gRNA 2b to create OPA1-del_exon5
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)
Plasmid#226990PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2bDepositorInsertSpCas9 gRNA 1b to create OPA1-del_exon5
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87)
Plasmid#226988PurposepUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9)DepositorInsertSpCas9 gRNA A9 to create OPA1-R194G
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)
Plasmid#221329PurposeDox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transductionDepositorUseRetroviralTagsExpressionMutationG12VPromoterTRE3GAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
METTL3
Plasmid#155725PurposeFor use in RBP tethering screenDepositorInsertMETTL3 (METTL3 Human)
UseTagsV5-MCPExpressionMammalianMutationH330Q compared to BC052244, no mutations compared…PromoterEF-1aAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pIG-724pUC19-tNGFR-P2A-EVER2
Plasmid#186105PurposeKnockin of truncated NGFR to target geneDepositorInsertEVER2 (TMC8 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-734pUC19-tNGFR-P2A-PIK3R1
Plasmid#186107PurposeKnockin of truncated NGFR to target geneDepositorInsertPIK3R1 (PIK3R1 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-713pUC19-tNGFR-P2A-CD45
Plasmid#186109PurposeKnockin of truncated NGFR to target geneDepositorInsertCD45 (PTPRC Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-721pUC19-tNGFR-P2A-RhoH
Plasmid#186116PurposeKnockin of truncated NGFR to target geneDepositorInsertRHOH (RHOH Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-720pUC19-tNGFR-P2A-ZAP70
Plasmid#186120PurposeKnockin of truncated NGFR to target geneDepositorInsertZAP70 (ZAP70 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-735pUC19-tNGFR-P2A-PIK3CD
Plasmid#186087PurposeKnockin of truncated NGFR to target geneDepositorInsertPIK3CD (PIK3CD Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-723pUC19-tNGFR-P2A-EVER1
Plasmid#186088PurposeKnockin of truncated NGFR to target geneDepositorInsertEVER1 (TMC6 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-727pUC19-tNGFR-P2A-WASP
Plasmid#186089PurposeKnockin of truncated NGFR to target geneDepositorInsertWASP (WAS Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-714pUC19-tNGFR-P2A-SAP
Plasmid#186091PurposeKnockin of truncated NGFR to target geneDepositorInsertSAP (SH2D1A Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-728pUC19-tNGFR-P2A-WIP
Plasmid#186094PurposeKnockin of truncated NGFR to target geneDepositorInsertWIP (WIPF1 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-729pUC19-tNGFR-P2A-Moesin
Plasmid#186095PurposeKnockin of truncated NGFR to target geneDepositorInsertMOESIN (MSN Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-742pUC19-tNGFR-P2A-Caspase8
Plasmid#186099PurposeKnockin of truncated NGFR to target geneDepositorInsertCASPASE8
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-705pUC19-tNGFR-P2A-STAT5b
Plasmid#186103PurposeKnockin of truncated NGFR to target geneDepositorInsertSTAT5B (STAT5B Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-459 tNGFR-P2A-STAT6
Plasmid#186070PurposeKnockin of truncated NGFR to target geneDepositorInsertSTAT6 (STAT6 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-460 tNGFR-P2A-STAT4
Plasmid#186075PurposeKnockin of truncated NGFR to target geneDepositorInsertSTAT4 (STAT4 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-458 tNGFR-P2A-STAT2
Plasmid#186076PurposeKnockin of truncated NGFR to target geneDepositorInsertSTAT2 (STAT2 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-455 tNGFR-P2A-WAS
Plasmid#186082PurposeKnockin of truncated NGFR to target geneDepositorInsertWAS (WAS Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22UbhaEng2aE4PCh(#271)
Plasmid#184085Purposecyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenicDepositorInsert3HA-ZfEng2a-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22UbhaEng2bE4PCh(#272)
Plasmid#184086Purposecyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenicDepositorInsert3HA-ZfEng2b-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22UbHEn2E4PCh(#262)
Plasmid#184084Purposecyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenicDepositorInsert3HA-chkEn2-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-DDX43-NT-G46D
Plasmid#134588PurposeExpress human DDX43 N-terminal region (1-253 aa, with G46D mutation) in E. coliDepositorInsertDDX43-N-terminal (1-253 aa) with G46D mutation (DDX43 Human)
UseTagsHisExpressionBacterialMutationfull-length (G154D)PromoterT7Available sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVE-FH-Dnd1-IN
Plasmid#70072Purpose2nd gen lentiviral vector. Cre-removable and tet/dox-controllable expression of FLAG-HA-tagged murine Dnd1 in mammalian cells.DepositorInsertDnd1 (Dnd1 Mouse)
UseCre/Lox and LentiviralTagsFLAG and HAExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa
Plasmid#233036PurposeTo express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoterDepositorInsertRfxCas13d-compatible gRNA expression cassette and hfCas13d
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pT7CFE1_polio_3ABC
Plasmid#212986PurposeEncodes Poliovirus type I Mahoney 3ABC optimized for expression in mammalian transcription/translation extractsDepositorInsertPoliovirus Type I Mahoney 3ABC
UseMammalian cell free protein expressionTagsHAExpressionMutationPromoterT7Available sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-P10HA
Plasmid#1345DepositorInsertP10 (CDK5R1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-hMYF5-3xHA (#732)
Plasmid#78332PurposeDoxycycline-inducible lentiviral overexpression of human MYF5 ORF with a 3xHA tagDepositorInsertMYF5 (MYF5 Human)
UseLentiviralTags3x HA tagExpressionMutationPromoterTRE2Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only