We narrowed to 11,805 results for: NSI;
-
Plasmid#91894PurposesgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.DepositorInsertKras sgRNA
UseLentiviralPromoterU6Available SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_nV5-TAOK2
Plasmid#173000Purposeconstitutive expression of V5-tagged TAOK2DepositorAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_GSK3B
Plasmid#111687PurposeMAC-tagged gene expressionDepositorInsertGSK3B (GSK3B Human)
ExpressionMammalianAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM60-S6K1(T389S) barcoded
Plasmid#48800Purposeexpresses mutant S6K1DepositorInsertS6K1 T389S (Rps6kb1 Rat)
UseLentiviralTagsFLAG and barcode: GGTACCExpressionMammalianMutationT389SPromoterCMVAvailable SinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV epArcLight
Plasmid#85803Purposegenetically-encoded fluorescent voltage indicatorDepositorInsertepArcLight
ExpressionMammalianMutationCi-VSP contains R217Q mutation; ecliptic pHluorin…PromoterCMVAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SASH3-1/1
Plasmid#91434PurposeProtein expression and purification of human SH3 domain construct SASH3-1/1DepositorInsertSASH3-1/1 (SASH3 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP Snail 8SA
Plasmid#16229DepositorInsertSnail (SNAI1 Human)
TagsEGFPExpressionMammalianMutation8SA: S97A, S101A, S105A, S108A, S112A, S116A, S12…Available SinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
H2B-mScarlet- hRec8(297-506)-hMeikin(1-332, E151R, R154E)- mNeonGreen
Plasmid#174716PurposeStable expression of fluorescent Separase cleavage sensor with human Rec8 fused to human MeikinDepositorInsertRec8 (REC8 Human)
UseRetroviralTagsH2B-mScarlet and mNeonGreenExpressionMammalianMutationhRec8: 297-506; hMeikin: 1-332, E151R, R154EAvailable SinceOct. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GSDMD_C192A-Myc
Plasmid#201168PurposeExpression of GSDMD gene in mammalian cells by retroviral transductionDepositorAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Nanog-CreER targeting construct
Plasmid#59720PurposeCre-ER knockin targeting construct for mouse endogenous Nanog locusDepositorInsertcreERT2
UseMouse TargetingPromotermNanogAvailable SinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGB2alpha2 35s:hCas9:tNos (GB0639)
Plasmid#68222PurposeTranscriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoterDepositorInserthCas9
UseCRISPR and Synthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-ARAF_S214C
Plasmid#51077Purposemammalian expression of ARAF S214CDepositorAvailable SinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
DsRed +9e
Plasmid#191766PurposeExpression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9eDepositorInsertDsRed
TagsN terminal fusion of positively charged polypetod…ExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SORBS1-1/3
Plasmid#91547PurposeProtein expression and purification of human SH3 domain construct SORBS1-1/3DepositorInsertSORBS1-1/3 (SORBS1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SORBS1-3/3
Plasmid#91553PurposeProtein expression and purification of human SH3 domain construct SORBS1-3/3DepositorInsertSORBS1-3/3 (SORBS1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SORBS1-2/3
Plasmid#91530PurposeProtein expression and purification of human SH3 domain construct SORBS1-2/3DepositorInsertSORBS1-2/3 (SORBS1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer
Plasmid#86195PurposeU6 based expression of N meningitidis sgRNADepositorInsertnmCas9 sgRNA cloning cassete
UseLentiviralPromoterU6Available SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_BET1
Plasmid#111683PurposeMAC-tagged gene expressionDepositorInsertBET1 (BET1 Human)
ExpressionMammalianAvailable SinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_FBRL
Plasmid#111686PurposeMAC-tagged gene expressionDepositorInsertFBRL (FBL Human)
ExpressionMammalianAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmalc4x_E323K_TEV
Plasmid#47699PurposeExpresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation E323KDepositorInsertMaltose Binding Protein and Olfactomedin-like domain with TEV cleavage site (MYOC Human)
TagsMBPExpressionBacterialMutationchanged glutamic acid 323 to lysinePromoterPtacAvailable SinceSept. 9, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA FLAG mTOR ST
Plasmid#22999DepositorAvailable SinceMay 18, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEPQD1CB0107
Plasmid#177040PurposeMoClo Level 1, position 1, transcriptional unit for transient expression of 1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptideDepositorInsert1-deoxy-D-xylulose 5-phosphate synthase (CrDXS2) from Catharanthus roseus
UseSynthetic BiologyAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-3xMyc-ERK2(K52R)
Plasmid#129228PurposeEncodes Myc tagged human ERK2(K52R) for purification by GST affinityDepositorInsertERK2(K52R) (Mapk1 Rat)
TagsGST and MycExpressionBacterialMutationChanged Lysine 52 to ArginineAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miR30_shBrd9_1116-PGK-NeoR-IRES-mCherry
Plasmid#75133PurposeRetroviral expression plasmid encoding shBrd9_1116 (targeting murine Brd9)DepositorInsertshBrd9_1116
UseRetroviralAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEJS542-pCSDest2-AcrIIC5Smu
Plasmid#113435PurposeExpresses type II-C anti-CRISPR protein from S. muelleri in mammalian cellsDepositorInsertType II-C anti-CRISPR
UseCRISPRExpressionMammalianPromoterCMVAvailable SinceDec. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP Snail 4SA
Plasmid#16227DepositorAvailable SinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
-
pEX-CFP-hLC3ΔG
Plasmid#24986DepositorInserta mutant human MAP1-LC3B fused with ECFP (MAP1LC3B Human)
TagsECFPExpressionMammalianMutationdeleted Gly at 121Available SinceJune 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA GAL4 CREB 1-341 M1
Plasmid#22973DepositorInsertCREB (Creb1 Rat, GAL4-fusion)
TagsGAL4 DNA-binding domainExpressionMammalianMutationChanged Serine 133 to Alanine of CREBAvailable SinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
GFP Snail 2A4E
Plasmid#16231DepositorInsertSnail (SNAI1 Human)
TagsEGFPExpressionMammalianMutation2A4E: S97A, S101A, S108E, S112E, S116E, S120EAvailable SinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:mVenus
Plasmid#133970PurposeHeterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.DepositorInsertmVenus
UseSynthetic BiologyExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXW117As(RS-E11)
Plasmid#123145PurposeArsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ117, ParsR, PhrpLE, Pecf11Available SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
LacI/109-pHG165c
Plasmid#90058PurposeWT LacI polymorphism with T at position 109DepositorInsertLacI/109
ExpressionBacterialMutation109PromoterIqAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVL94 - pOp4:VND7
Plasmid#116016Purposedestination vector for GreenGate cloning method, "Effector line", crossed with a "Driver line" the GOI, here VND7, will be expressed upon Dex-inductionDepositorInsertpOp4:B-dummy:VND7:VP16:tUBQ10::BastaR
ExpressionPlantAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_RAB11A
Plasmid#111691PurposeMAC-tagged gene expressionDepositorInsertRAB11A (RAB11A Human)
ExpressionMammalianAvailable SinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
Flag Snail 4SE
Plasmid#16223DepositorAvailable SinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCA-G-intron(Neo)-T(FLLFLNL)
Plasmid#40020DepositorInsertsGFP
tdT-3Myc
beta-globin intron
Neo
Tags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta globin promoter and CMV enhance…Available SinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOPTXcGMPRELUC
Plasmid#68503Purposecyclic nucleotide reporter for bacteria or plant cellsDepositorInsertOPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
TagsluciferaseExpressionBacterial and PlantMutation3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…PromoterOPTX promoter with cGMPREAvailable SinceSept. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal TDP43 G294A
Plasmid#27459DepositorAvailable SinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
phumanCas9 (GB0575)
Plasmid#68206PurposeProvides the human codon optimized CDS of Cas9 protein as a level 0 GoldenBraid partDepositorInsertCas9 coding region (human codon optimised)
UseCRISPR and Synthetic BiologyMutationBsaI and BsmBI sites removed; human codon optimis…Available SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only