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We narrowed to 81,735 results for: TRI

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Showing: 6481 - 6520 of 81735 results

  2. pOPINB-hRNF216(457-784)

    Plasmid
    #171922
    Purpose
    Expression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.
    Depositor
    Bernhard Lechtenberg
    Article
    Cotton et al Mol Cell. 2022 Feb 3;82(3):598-615.e8. d
    Insert
    E3 ubiquitin-protein ligase RNF216 (RNF216 Human)
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    codon optimised for expression in E. coli
    Promoter
    T7
    Available Since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    171922-plasmid-map-sequence-id-346327

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  10. pAcBac1-Mb-Fluoro-Phe D6

    Plasmid
    #197577
    Purpose
    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10
    Inserts
    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    Tags
    Nuclear export sequence + FLAG
    Expression
    Mammalian
    Mutation
    N311A, C313M, V366G, W382T
    Promoter
    CMV and U6/H1
    Available Since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197577-plasmid-map-sequence-id-388240

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  12. pC-SUR-YR

    Plasmid
    #61768
    Purpose
    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing sulfonylurea resistance gene (ILV alelle from Magnaporthe oyrzae) on transfer DNA (TDNA).
    Depositor
    Ken Haynes
    Article
    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do
    Inserts
    Sur gene
    2 micron origin or replication and URA3 gene for S. cerevisiae
    Use
    Ternary vector for agrobacterium mediated transfo…
    Promoter
    Magnaporthe oryzae ILV promoter
    Available Since
    May 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    61768-plasmid-map-sequence-id-442790

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  13. pAcBac1-Ma-sfGFP 150TAG

    Plasmid
    #197568
    Purpose
    Expression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP 150TAG
    M. alvus Pyl-tRNA (6) (4x copies)
    Tags
    V5-His6
    Expression
    Mammalian
    Mutation
    N150TAG
    Promoter
    CMV and U6/H1
    Available Since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197568-plasmid-map-sequence-id-388231

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  18. pMTB2-Avi-Cerulean-RanGAP

    Plasmid
    #79878
    Purpose
    Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    Tags
    Avi
    Expression
    Bacterial
    Promoter
    Beta-actin2
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79878-plasmid-map-sequence-id-149739

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  22. pAcBac1-Mb-Fluoro-Phe B5

    Plasmid
    #197576
    Purpose
    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe B5 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10
    Inserts
    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    Tags
    Nuclear export sequence + FLAG
    Expression
    Mammalian
    Mutation
    N311G, C313A
    Promoter
    CMV and U6/H1
    Available Since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197576-plasmid-map-sequence-id-388235

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  25. pGST-HsSyx-375-792

    Plasmid
    #179292
    Purpose
    E. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 375-792) with N-terminal His6 + GST + TEV protease cleavage site
    Depositor
    Debra Hansen
    Article
    Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.
    Insert
    Syx
    Tags
    His6 + GST + TEV protease cleavage site
    Expression
    Bacterial
    Mutation
    residues 375-792 from accession number NP_0010361…
    Promoter
    T7
    Available Since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    179292-plasmid-map-sequence-id-354918

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  28. pMT-ubb-Avi-Cerulean-Rpl10a

    Plasmid
    #79882
    Purpose
    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    Tags
    Avi
    Expression
    Bacterial
    Promoter
    Ubiquitin
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79882-plasmid-map-sequence-id-149785

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Showing: 6481 - 6520 of 81735 results