We narrowed to 26,673 results for: Cat

1. pET-His-SCov2-nsp8

   Plasmid

   #154758

   Purpose

   Expression of SARS-CoV-2 nsp8

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FLAG-MRGBP

   Plasmid

   #15325

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Cai et al J Biol Chem. 2003 Oct 31. 278(44):42733-

   Insert

   MRG-binding protein (MRGBP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pTorPE-jREX-GECO1

   Plasmid

   #113941

   Purpose

   Bacterial expression of red fluorescent Ca2+ sensor jREX-GECO1

   Depositor

   Robert Campbell, Thomas Hughes

   Article

   Molina et al Biophys J. 2019 May 21;116(10):1873-1886

   Insert

   jREX-GECO1
   

   Use

   Tags

   6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTP341 pHAGE2 CMV TagBFP-3xFLAG

   Plasmid

   #199391

   Purpose

   Lentiviral transfer plasmid for constitutive expression of a TagBFP-3xFLAG in mammalian cells

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   TagBFP-3xFLAG
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. His6_MBP_FXR2P_P474S_P493N

   Plasmid

   #198695

   Purpose

   Expression of human FXR2 protein in E. coli

   Depositor

   Simpson Joseph

   Article

   Edwards et al Sci Rep. 2020 Sep 28;10(1):15858. doi: 1

   Insert

   FXR2P (FXR2 Human)
   

   Use

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   Changed proline 474 to serine and proline 493 asp…

   Promoter

   T7

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDAS12125_PEAR-GFP

   Plasmid

   #177178

   Purpose

   PEAR-GFP target plasmid with split EGFP and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Insert

   EGFP split with an intron between amino acids 95-96
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHiUGE HA-epitope donor ORF2

   Plasmid

   #200373

   Purpose

   Tagging endogenously expressed proteins with HA epitope, C-terminal

   Depositor

   Scott Soderling

   Article

   Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

   Insert

   HA epitope
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-UBC9-K14R-A129K

   Plasmid

   #228948

   Purpose

   express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)

   Depositor

   Marcin Suskiewicz

   Insert

   UBC9 (UBE2I Human)
   

   Use

   Tags

   His tag and thrombine cleavage site : MGHHHHHHSSG…

   Expression

   Bacterial

   Mutation

   changed Lysine 14 to Arginine, changed Alanine 12…

   Promoter

   T7-lac

   Available since

   May 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-hsyn-flex-Positron-ST

   Plasmid

   #129267

   Purpose

   Cre-dependant expression of soma-localized Positron in neurons

   Depositor

   Eric Schreiter

   Article

   Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

   Insert

   Positron-ST
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   D92N, E199V

   Promoter

   hsyn

   Available since

   Oct. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SSB-wt

    Plasmid

    #197260

    Purpose

    wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

    Depositor

    Timothy Lohman

    Article

    Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

    Insert

    wt E. coli SSB
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. RIP Cre-ER (DM#265)

    Plasmid

    #15029

    Depositor

    Douglas Melton

    Article

    Dor et al Nature. 2004 May 6. 429(6987):41-6.

    Insert

    Rat insulin promoter (RIP) (Ins2 Rat)
    

    Use

    Cre/Lox; For making transgenic constructs

    Tags

    Cre-ER (TM)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. DNA-PKcs N-terminal sgRNA

    Plasmid

    #207093

    Purpose

    pX330 based plasmid for expression of Cas9 and the AGCGGGACTCGGCGGCATGG sgRNA to target the DNA-PKcs locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    AGCGGGACTCGGCGGCATGG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hMTP

    Plasmid

    #124281

    Purpose

    Expresses human MTP in mammalian cells.

    Depositor

    Mahmood Hussain

    Article

    Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

    Insert

    MTTP (MTTP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLH-sgRNA-Sirius-4X(MS2-PP7)

    Plasmid

    #121941

    Purpose

    CRISPR-Sirius plasmid

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

    Insert

    sgRNA-Sirius-4X(MS2-PP7)
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFW2000

    Plasmid

    #224211

    Purpose

    E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP-hTimeless (pc4729)

    Plasmid

    #216861

    Purpose

    Mammalian expression vector encoding GFP tagged human Timeless

    Depositor

    Cristina Cardoso

    Article

    Prorok et al Front Cell Dev Biol. 2024 Feb 29;12:1346

    Insert

    Timeless (TIMELESS Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p210 pCMV-CREM

    Plasmid

    #8395

    Purpose

    Cre with chimeric/β-globin intron within the Cre coding sequence

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    CMV

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSB700 lenti gRNA mcherry

    Plasmid

    #167910

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p-G4PID-HA

    Plasmid

    #209639

    Purpose

    Expression G4PID-HA in mammalian cells

    Depositor

    Xiang Zhou

    Article

    Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

    Insert

    G4PID (DHX36 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p6610 MSCV-IP N-HAonly TIMP1

    Plasmid

    #35009

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    TIMP1 (TIMP1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    March 2, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pCMV MDM-2

    Plasmid

    #16441

    Depositor

    Bert Vogelstein

    Article

    Oliner et al Nature. 1992 Jul 2. 358(6381):80-3.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRSFDuet1-Cdc45

    Plasmid

    #126878

    Purpose

    Expresses human Cdc45 in bacteria

    Depositor

    Luca Pellegrini

    Article

    Simon et al Nat Commun. 2016 May 18;7:11638. doi: 10

    Insert

    CDC45 (CDC45 Human)
    

    Use

    Tags

    No tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Human NCKX2-AS-long-FlagTag (pcDNA3.1+)

    Plasmid

    #75203

    Purpose

    Mammalian expression of SLC24A2

    Depositor

    Jonathan Lytton

    Article

    Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

    Insert

    SLC24A2 (SLC24A2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. groSL-GFP

    Plasmid

    #109389

    Purpose

    GFP cassette under the control of the groSL sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pgroSL-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Nano-lantern(cAMP-1.6)/pRSETB

    Plasmid

    #53591

    Purpose

    Nano-lantern-based luminescent cAMP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern-based luminescent cAMP indicator
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

26. pET28a-Hook3 aa 1-552

    Plasmid

    #74614

    Purpose

    Expresses a truncation of Hook3 in bacteria for purification

    Depositor

    Ron Vale

    Article

    Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

    Insert

    human Hook3 amino acids 1-552 (HOOK3 Human)
    

    Use

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pQTEV-FLJ30046

    Plasmid

    #34828

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    FLJ30046 (SLAIN1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAUC40

    Plasmid

    #71298

    Purpose

    Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

    Depositor

    Susanne von Bodman

    Article

    Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMUT2-pTcpP-mCherry

    Plasmid

    #192861

    Purpose

    pMUT2 based vector for sensing human primary bile acids in E. coli Nissle 1917

    Depositor

    Morten Sommer

    Article

    Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

    Insert

    TcpP-CadC fusion protein and cognate promoter inducible by human primary bile acids
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Nano-lantern(cAMP-1.6)/pcDNA3

    Plasmid

    #53594

    Purpose

    Nano-lantern-based luminescent cAMP indicator

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern-based luminescent cAMP indicator
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

31. Fus-BcLOV-mCh-SOS

    Plasmid

    #208275

    Purpose

    Expresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

    Depositor

    Lukasz Bugaj

    Article

    Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

    Insert

    Fus-BcLOV-mCh-SOS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA5-FRT-TO-N-Flag-8His

    Plasmid

    #157677

    Purpose

    N-terminal tagging vector

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    Flag-8His
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pcDNA5-FRT-TO-eGFP

    Plasmid

    #157733

    Purpose

    mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. miniCMV eGFP hPGK BSD

    Plasmid

    #188519

    Purpose

    Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AsdCas12a KRAB

    Plasmid

    #188502

    Purpose

    Expresses FLAG tagged AsdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    EF1a

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pH6HTC_HSPA5-Halo

    Plasmid

    #175326

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    HSPA5 (HSPA5 Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pLenti-CaMKIIa-C1C2-EYFP

    Plasmid

    #35519

    Depositor

    Karl Deisseroth

    Article

    Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

    Insert

    ChR1-ChR2 Chimera
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available since

    June 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. enz.083-NMT1

    Plasmid

    #154047

    Purpose

    Expresses NMT1 in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    NMT1 (N-myristoyltransferase 1) (NMT1 Human)
    

    Use

    Tags

    His, MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. ibpAB-GFP

    Plasmid

    #109390

    Purpose

    GFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    pibpAB-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. NAX_CAGGS_CAS9_VPR_Puro

    Plasmid

    #167895

    Purpose

    PiggyBac compatible plasmid expressing Cas9-VPR

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pSB700 lenti gRNA EYFP

    Plasmid

    #167906

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. mTurquoise2-3xrGBD

    Plasmid

    #176100

    Purpose

    Visualization of active Rho

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    rhotekin (Rtkn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. WASp(CRIB)-HaloTag-WASp(CRIB)

    Plasmid

    #191452

    Purpose

    Visualizing endogenous & active Cdc42

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    WASp
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. scFv-IFP2.0N

    Plasmid

    #215746

    Purpose

    Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    IFP2.0N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. 12URA-B-ScTop2∆CTD

    Plasmid

    #214924

    Purpose

    Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

    Depositor

    James Berger

    Article

    Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

    Insert

    topoisomerase II
    

    Use

    Tags

    6x His-TEV

    Expression

    Yeast

    Mutation

    Deleted amino acids 1178-1428

    Promoter

    Gal 1/10

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. htpG1-GFP

    Plasmid

    #109387

    Purpose

    GFP cassette under the control of the htpG1 sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.

    Depositor

    Tom Ellis

    Article

    Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

    Insert

    phtpG1-GFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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47. Mito-Lapronic/pGW1

    Plasmid

    #140754

    Purpose

    FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

    Depositor

    Luis Felipe Barros

    Article

    Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

    Insert

    Mito-Lapronic
    

    Use

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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48. pDonor14-ShoHELIX-KanR (BO4)

    Plasmid

    #181795

    Purpose

    pDonor for ShoHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    KanR, R6K origin of replication
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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49. pGEX-Rac1

    Plasmid

    #12200

    Depositor

    Jonathan Chernoff

    Article

    Bagrodia et al J Biol Chem. 1995 Sep 29. 270(39):22731-

    Insert

    Rac1 (RAC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Starting methionine (M1) removed; F78S compared t…

    Promoter

    Available since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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50. pUC Ad VAI

    Plasmid

    #175518

    Purpose

    For mammalian expression of adenovirus VAI RNA

    Depositor

    Britt Glaunsinger

    Article

    Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

    Insert

    Ad VAI RNA
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    lac promoter

    Available since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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