We narrowed to 7,518 results for: Lif

1. Lenti-guide-puro mBltp1 (KIAA1109) - 1

   Plasmid

   #198489

   Purpose

   lentiviral stable expression of mBltp1 (KIAA1109) gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mBltp1 (4932438A13Rik Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-guide-puro mBltp1 (KIAA1109) - 2

   Plasmid

   #198490

   Purpose

   lentiviral stable expression of mBltp1 (KIAA1109) gRNA 2

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mBltp1 (4932438A13Rik Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Lenti-guide-puro mAtp6v1a-1

   Plasmid

   #198477

   Purpose

   lentiviral stable expression of mAtp6v1a gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mouse ATPv1a (Atp6v1a Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Lenti-guide-puro mAtp6v1a-2

   Plasmid

   #198478

   Purpose

   lentiviral stable expression of mAtp6v1a gRNA 2

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mouse ATPv1a (Atp6v1a Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGBT-RP2.2

   Plasmid

   #189842

   Purpose

   Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 2.

   Depositor

   Stephen Ekker

   Article

   Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   GFP GM2 variant and mRFP

   Expression

   Mutation

   Promoter

   beta Actin

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGBT-RP2.3

   Plasmid

   #189843

   Purpose

   Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Ubiquitous GFP frameshift 3.

   Depositor

   Stephen Ekker

   Article

   Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   GFP GM2 variant and mRFP

   Expression

   Mutation

   Promoter

   beta Actin

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBT-RP8.3

   Plasmid

   #189848

   Purpose

   Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 3.

   Depositor

   Stephen Ekker

   Article

   Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   mRFP and tagBFP

   Expression

   Mutation

   Promoter

   beta Actin

   Available since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. kanMX6-ins1

   Plasmid

   #195038

   Purpose

   pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   KanR
   

   Use

   Yeast genomic targeting

   Tags

   S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-sgCtrl_EF1a-Puro-T2A-BFP

   Plasmid

   #195500

   Purpose

   Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence

   Depositor

   Alexander Meissner

   Article

   Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

   Insert

   sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP, Puro, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-sgT-REX17_EF1a-Puro-T2A-BFP

    Plasmid

    #195501

    Purpose

    Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17

    Depositor

    Alexander Meissner

    Article

    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP, Puro, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV_Syn_Xph18_eGFP_CCR5TC

    Plasmid

    #187443

    Purpose

    Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph18
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV_Syn_Xph15_eGFP_CCR5TC

    Plasmid

    #187442

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. fru-T2A-CsChrimson-tdTomato HDR plasmid

    Plasmid

    #141100

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses optogenetic activator CsChrimson-tdTomato by knocking-in fragment into the coding sequence of the endogenous Aedes aegypti fru gene

    Depositor

    Leslie Vosshall

    Article

    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

    Insert

    fru-left-HDR-arm; T2A-CsChrimson-tdTomato-SV40; 3xP3-EYFP-SV40; fru-right-HDR-arm
    

    Use

    Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUGW-shRIIα-RIIαS97A-IRES-EGFP

    Plasmid

    #183457

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97A variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFUGW-shRIIα-RIIαS97E-IRES-EGFP

    Plasmid

    #183458

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with the RIIα S97E variant

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJET-22-DExCon-mNeonGreen-R11a

    Plasmid

    #179897

    Purpose

    Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Tags

    mNeonGreen

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMH011_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid

    #179434

    Purpose

    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-HS4-mch (SH)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL006_phiC31_neo-5xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

    Plasmid

    #179425

    Purpose

    Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration

    Depositor

    Lacra Bintu

    Article

    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

    Insert

    TRE-cit-(nospacer)-mch
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pEF alpha, pRSV

    Available since

    Feb. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV9-hSyn-iDianiSnFR_PM

    Plasmid

    #177759

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pAAV9-hSyn-iDianiSnFR_PM
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV9-hSyn-iDianiSnFR_ER

    Plasmid

    #177758

    Purpose

    Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

    Depositor

    Henry Lester

    Article

    Nichols et al eLife. 2022 Jan 4;11. pii: 74648. doi: 1

    Insert

    pAAV9-hSyn-iDianiSnFR_ER
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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